- Study protocol
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Common beans and cowpeas as complementary foods to reduce environmental enteric dysfunction and stunting in Malawian children: study protocol for two randomized controlled trials
Trialsvolume 16, Article number: 520 (2015)
Interventions to decrease the burden of childhood malnutrition are urgently needed, as millions of children die annually owing to undernutrition and hundreds of millions more are left cognitively and physically stunted. Environmental enteric dysfunction (EED), a pervasive chronic subclinical inflammatory condition among children that develops when complementary foods are introduced, places them at high risk of stunting, malabsorption, and poor oral vaccine efficacy. Improved interventions to reduce the burden of EED and stunting are expected to markedly improve the nutritional status and survival of children throughout resource-limited settings.
We will conduct, in parallel, two prospective randomized controlled clinical trials to determine whether common beans or cowpeas improve growth, ameliorate EED, and alter the intestinal microbiome during a high-risk period in the lives of rural Malawian children. Study 1 will enroll children at 6 months of age and randomize them to receive common beans, cowpeas, or a standard complementary food for 6 months. Anthropometry will be compared among the three groups; EED will be assessed using a dual-sugar absorption test and by quantifying human intestinal mRNA for inflammatory messages; and the intestinal microbiota will be characterized by deep sequencing of fecal DNA, to enumerate host microbial populations and their metabolic capacity. Study 2 will enroll children 12–23 months old and follow them for 12 months, with similar interventions and assessments as Study 1.
By amalgamating the power of rigorous clinical trials and advanced biological analysis, we aim to elucidate the potential of two grain legumes to reduce stunting and EED in a high-risk population. Legumes have potential as an affordable and effective complementary food intervention, given their cultural acceptability, nutritional content, and agricultural feasibility in sub-Saharan Africa.
Approximately 45 % of all deaths worldwide among children under the age of five, that is, 3.1 million deaths annually, are directly or indirectly related to undernutrition . Additionally, stunting permanently affects an additional 165 million children worldwide, and reduces the affected individual’s physical, immunological, and cognitive capacity throughout his or her lifetime. Stunting is estimated to account for 21 % of all disability-adjusted life years in children. Both stunting and wasting are causally related to the dietary intake and gut health in children younger than 3 years.
Environmental enteric dysfunction
In developing, impoverished settings, a common gut inflammatory condition known as environmental enteric dysfunction (EED, formerly known as environmental enteropathy, and even earlier as tropical enteropathy) develops early in life [2, 3]. While subclinical, EED predisposes children to more clinically manifest forms of malnutrition: wasting and stunting. Environmental enteric dysfunction is characterized by T-cell infiltration of the intestinal mucosa, leading to a chronic inflammatory state with increased intestinal permeability, translocation of gut microbes, micro- and macronutrient malabsorption, poor weight gain, stunted physical and cognitive development, frequent enteric infections, and decreased response to enteric vaccines . While a precise etiology of EED has not been identified, it is epidemiologically associated with unsanitary living conditions . Given the significant contribution of malnutrition to childhood morbidity and mortality, meaningful progress on reducing EED might establish a lasting foundation for progress against global hunger [6, 7].
Environmental enteric dysfunction develops within the first three years of life, a high-risk period marked also by the transitions from exclusive breastfeeding to mixed feeding with complementary foods and then to complete reliance on adult foods for sustenance . In traditional sub-Saharan African societies, complementary foods are dominated by monotonous, protein-poor, micronutrient-poor starches, such as maize, cassava, and sorghum. Alternative, yet culturally acceptable, complementary foods that can provide a better balance of nutrients might decrease EED and improve growth.
Legumes provide such an opportunity, as their protein content is significantly higher than cereals, and they are rich in dietary fiber, starch, minerals, vitamins, and antioxidants. Common beans and cowpeas, for example, have 3–4-fold more protein per gram than corn. The zinc content in legumes is also relatively high and might further decrease the progression of EED, as we have demonstrated recently in a prospective randomized trial . Legumes make an excellent complementary food for children weaning from exclusive breastfeeding and, with appropriate preparation, are quite digestible and well-tolerated . Successful legume–maize blends have, in fact, already been developed in the past and demonstrated favorable acceptability profiles in children younger than one year; they were also nutritionally sound as a weaning supplement .
Additionally, interventions with anti-inflammatory effects might improve gut health, since EED is a chronic inflammatory condition, both in the lamina propria of the intestinal tract and systemically, owing to translocation of enteric bacteria and their products across the compromised mucosal border . Simply attacking the bacterial confounders of EED alone is unlikely to be sufficient, given past failures to improve EED via the use of probiotics  or antibiotics . A growing body of evidence [14–17] suggests that a diet enriched in legumes decreases markers of inflammation and is correlated with decreases in illnesses with inflammatory components such as colorectal cancer and cardiovascular disease .
Evidence from molecular and animal models supports a mechanistic explanation whereby certain legumes may have direct anti-inflammatory effects on the intestines of children with EED. For example, the indigestible portion of common beans downregulates signaling pathways that lead to inflammation in a mouse model of colon cancer . Intriguingly, there is probably an essential microbial component to the downregulation of this inflammatory cascade: when fermented by normal gut flora, the indigestible fraction from common bean produces short-chain fatty acids that inhibit colon cancer HT-29 cell growth and modulates protein expression associated with apoptosis, cell cycle arrest, and proliferation. These effects were also demonstrated morphologically . The indigestible fraction of common beans also protects against chemically induced crypt inflammation in the rat colon [21, 22].
Cowpeas (Vigna unguiculata) are also attractive for study, as they grow well in Africa, are culturally accepted, and are a hardy, drought-tolerant, crop. Cowpeas also have significant anti-inflammatory effects, mediated by specific phenolic profiles and antioxidant activity . These phenolic compounds are also active after cooking and simulated enzymatic digestion .
The evolving intestinal microbiome in African children also warrants study. Although no specific microbial populations or disruptions have yet been linked to EED , evidence suggests that a disruption in the relative populations among the four dominant bacterial phyla (Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria) are correlated with gut mucosal breakdown in inflammatory bowel disease [26, 27]. We have demonstrated recently that empiric antimicrobial interventions in severely malnourished children improve nutritional recovery , and that specific disturbances in the maturation of young children’s intestinal microbiota are linked to severe malnutrition . Hence, it would be instructive to understand the effect that specific food-based interventions have on commensal gut organisms and their metabolic capacity . A recent study comparing the gut microbiota in children from rural Burkina Faso, who consumed a diet rich in legumes, with European children showed a relative lack of potentially pathogenic Enterobacteriaceae in the African children, conceivably protecting these children from severe gut inflammation and bacterial translocation .
In our previous work, we have found EED in more than 80 % of children studied in rural Malawi [8, 12, 13, 32]. In these studies, we plan to investigate the effects on childhood growth, gut health, and the intestinal microbiome caused by the systematic introduction of cowpeas and common beans into the complementary diet of vulnerable Malawian children.
In parallel, we will conduct two prospective, randomized, investigator-blinded, controlled trials to compare the effectiveness of legume consumption with the customary local complementary food on infant and toddler growth and gut health in rural Malawi. Study 1 will enroll children at 5.5–6.5 months of age and provide the randomized intervention for 6 months. Study 2 will enroll children at 12–23 months of age and provide the randomized intervention for 12 months. A probable patient flow diagram for each trial is shown in Fig. 1.
The studies will enroll children in the village clusters surrounding Limela, Machinga District, and Masenjere, Nsanje District, Malawi. These villages are populated primarily by subsistence farmers who grow and consume maize as their primary staple crop. Water is generally acquired several times per day from boreholes or nearby streams. Most homes are quite small and are constructed from mud bricks and have thatch, or occasionally sheet metal, roofs. Defecation generally occurs outdoors or in small pit latrines. Children have frequent contact with farm animals, such as chickens and goats. Electricity, running water, and other utilities are invariably absent in homes. Almost all household chores and child-rearing is performed by mothers or other female figures; in these villages, most of the women are illiterate and it is quite rare for women to have completed primary school.
We aim to complete the study using the highest ethical standards for clinical trials research, in accordance with the Declaration of Helsinki . Ethical approval has been obtained from the University of Malawi College of Medicine Research and Ethics Committee and from the Human Research Protection Office at Washington University in St. Louis. Letters of support have also been obtained from the district health officers in the two districts where the study will be conducted. Local village chiefs surrounding the study sites have also given their consent and support for the studies. Each child’s caregiver will be briefed on the study procedures and interventions. Verbal and written informed consent will be obtained from all caregivers. Caregivers who are unable to sign or write their names will be asked to document consent using their thumbprints.
Children living within walking distance of one of the two study sites and within the enrollment ages for the two studies (5.5–6.5 months for Study 1 or 12–23 months for Study 2) will be screened for eligibility. Children without severe or moderate acute malnutrition (defined as a weight-for-height Z score < −2, mid-upper arm circumference < 12.5, or pitting edema suggestive of kwashiorkor) and without evidence of congenital or other chronic diseases will be considered eligible. Additional exclusion criteria will include current treatment for acute malnutrition and any report from the caregiver that the child currently has diarrhea. Caregivers who report that they will be moving away from the study area during the course of the study (6 months for Study 1 or 12 months for Study 2) will also be excluded.
Primary outcome measures and sample size
The primary outcome measures of interest will be change in height-for-age Z score and improvement in the major biomarker of environmental enteropathy, the lactulose to mannitol ratio (L:M) . To detect a difference in change in length of 1.1 cm, which corresponds to a change in height-for-age Z score of 0.45 units at 12 months of age, 79 children are needed in each group. This sample size was calculated for a two-tailed test using G*Power 184.108.40.206  at a significance level of 0.05 and 80 % power.
We also desire to detect a (medium) effect size of 0.5 in the L:M test after the legume intervention, again with 80 % power at a significance level of 0.05. Given these parameters, if the L:M results were to follow a normal distribution, thereby allowing the use of Student’s t test to compare the differences between independent means, 64 children would be needed in each group. If the L:M results are not normally distributed and require nonparametric analysis with the Wilcoxon–Mann–Whitney test, then up to 74 children might be needed per group. In recent studies, we have experienced approximately a 5 % rate of insufficient urine specimens . We therefore very conservatively estimate that this rate might increase to 15–20 %. Thus, we believe that enrolling 300 children with a 15–20 % rate of failure to obtain sufficient urine (owing to dropouts and specimen collection failures) will yield complete analysis for at least 240 children, or 80 in each group.
Should 100 children indeed be enrolled and retained in each intervention arm, the power achieved for the height-for-age Z score criterion is 89 % and for the L:M criteria is 94 % (if normally distributed) and 93 % (if non-normally distributied).
Children in each study will be randomly assigned to receive common bean flour, cowpea flour, or corn–soy blended flour in a 1:1:1 ratio across both study sites. The legume flours will be produced from locally grown and harvested legumes available in local markets, after sorting to remove rotten beans and any other foreign materials. After trialing several different methods of cooking the legumes in small acceptability tests with local children, we determined that roasting and milling the beans into a fine flour to be added to traditional corn porridge meals was the most palatable and practical method for introducing these new foods to the diet. Corn–soy blended flour will be purchased from local suppliers and caregivers will also be taught to administer this control intervention in traditional porridge.
Children in Study 1 will receive approximately 40 % of their daily energy requirements from complementary feeding (200 kcal/day at 6–8 months of age; 300 kcal/day at 9–11 months of age)  via the intervention foods (Table 1). Given that the duration and amount of breastfeeding can be so variable in children after their first year of life, children in Study 2 will receive approximately 15–20 % of the estimated total daily energy requirement for nonbreastfed children [37, 38] via the intervention foods (Table 2).
Choice of legume intervention
The specific intervention legumes thus chosen were Andean white common beans (Phaseolus vulgaris) and cowpeas (Vigna unguiculata). The rationale for this choice was based on several considerations. We felt that the legumes chosen must be able to be grown widely by subsistence farmers in sub-Saharan Africa (that is, the families of children with EED) . Common beans and cowpeas met this criteria, as they are the most commonly grown legumes in Africa. Cowpeas, for example, grow well in marginal soils that are 85 % sand and less than 1 % organic matter, as is often found in Africa. As legumes, both also fix nitrogen in the soil rather than extracting it.
These two legumes are also good choices since their nutrient content adds diversity and quality to the habitual African diet, most notably in terms of protein content. Of course, the legumes chosen must not pose any significant health risks; common beans do pose a minor risk with phytohemagglutinin, but are otherwise considered to be very safe. White common beans were selected in part because their phytohemagglutinin content is one-third that of red common beans . The legumes chosen also support microbiota that produce short-chain fatty acids or microbiota that are associated with an anti-inflammatory effect [41, 42].
Preparation of legume flours for consumption
After hand-sorting of the common beans and cowpeas to remove rotten beans and those infected with weevils, beans will be roasted in 100 kg batches to an internal temperature of 120–130 °C for 45–50 minutes. This serves several purposes: (1) to improve palatability; (2) to decrease the risk of microbial contamination; and (3) to decrease the risk of phytohemagglutinin poisoning by denaturing the toxin found in raw beans. Roasted beans are then milled into a flour.
Caregivers of children enrolled in the study will be taught to prepare the flours by mixing the allotted amount with a small amount of unheated water and mix with the traditional maize porridge (‘phalla’) that is the traditional staple complementary food for children of this age. This porridge is traditionally prepared by adding maize flour to a pot of boiling water and stirring continuously for 10–15 minutes until the desired consistency is obtained. By not boiling the intervention flour, the quantities of nutrients and fibers in the flours will be unchanged from the amounts measured after production of the flours. We anticipate that this simple preparation method will also improve compliance, as caregivers do not need to cook anything extra for this one child – instead, a small portion of porridge can be set aside and the assigned intervention flour with water can be added to the already cooked maize porridge.
Randomization methods and blinding
Block randomization will be employed, using a computer-generated list in permuted blocks of nine. Allocation groups will be pre-printed on paper that will be sealed in opaque envelopes. The caregivers will then each draw out one opaque envelope, which will be opened in their presence, to determine their intervention groups.
Each type of flour will be packaged identically, except for the color of the lid used on the jars that contain the interventions. The allocation code connecting the lid colors to the types of flour is known only to the individuals involved in the packaging of the flours. All field research nurses, investigators, and laboratory personnel will be kept blinded to the color code as well as to each child’s assigned color throughout the study. Caregivers will not be told which type of flour they have been assigned, but while the flours have a very similar appearance and texture, it is reasonable to assume that caregivers may deduce the type of flour their children has been assigned by tasting or smelling the flour themselves. To reflect this possibility, we thus consider these to be investigator-blinded studies.
After obtaining verbal and written consent from caregivers of eligible children, randomization will occur and basic sociodemographic, food frequency, and economic data will be collected. Recent illnesses during the previous 7 days will be queried at each visit . At the initial visit, anthropometric data will be gathered to ensure that the child is not acutely malnourished and thus ineligible for the study. If eligible, the child will continue with the dual-sugar absorption test and stool collection. After successful completion of the dual-sugar absorption test and stool collection, a 6-week supply of the assigned flour intervention will be provided and the caregiver will be educated on how to prepare and feed the flour to the child. The complete schedule of study visits, testing, and distribution of flours is provided in Table 3 for Study 1 and Table 4 for Study 2.
Weight will be assessed to the nearest 5 g using standard digital scales (seca 334, Chino, CA, USA) and height will be measured to the nearest 0.1 cm using a rigid height board (seca 417, Chino, CA, USA). Mid-upper-arm circumference will be measured on each child’s left upper arm using standard flexible plastic insert tapes to the nearest 0.1 cm (TALC, St. Albans, UK). Each child will also be assessed for kwashiorkor by pressing with the pad of the thumb on the dorsum of the foot and observing for pitting edema. Any child with a weight-for-height Z score less than −2, a mid-upper arm circumference less than 12.5, or pitting edema will be classified as acutely malnourished . Malnourished children will be excluded from enrollment in the study and will instead be treated with ready-to-use lipid-nutrient spreads according to usual protocols until they recover .
Dual-sugar absorption test
The dual-sugar permeability test  will be used as a standard noninvasive measure of gut integrity, as it correlates with the severity of small bowel histological changes , severity of diarrhea , micronutrient status [48, 49], and growth in weight and length . The ratio of urinary excretion of lactulose to mannitol is used to measure intestinal mucosal permeability with higher ratios indicative of increased intestinal permeability.
To participate in the test, children will be asked to take no food (water and breast milk are permitted) after 10 p.m. on the previous night. The test begins when each child drinks a 20 ml solution containing 1 g mannitol and 5 g lactulose. Children will be carefully observed by research assistants to ensure complete ingestion of this solution; any child who spits up, vomits, or refuses the solution will be brought back for testing on another day. Adhesive urine bags will then be placed on the child’s perineum and monitored for urine output. As soon as urine is noted in the bag, it will be removed and the urine transferred to a clean container containing 10 mg merthiolate to prevent bacterial degradation of the excreted sugars, and a new bag will be placed. After 3–3.5 hours, children will be given water to drink to facilitate the collection of urine. After the child voids for the first time after 4 hours, the urine collection will be omplete. The total amount of urine voided during the study will then be quantified and an aliquot flash frozen for analysis.
The concentrations of lactulose and mannitol in the total voided urine will be determined using HPLC, as described previously . Given a recent report that HPLC is less reliable than liquid chromatography-tandem mass spectrometry , we previously compared our L:M results by HPLC on 115 samples with those obtained by the Mayo Clinic performed using liquid chromatography-tandem mass spectrometry and we found the results to be highly correlated (data not shown). The concentrations of these sugars in the urine will then be multiplied by the total amount of urine voided to provide a measure of the total amount of these sugars voided. The ratio of the sugars voided in the urine, as well as the amount voided as a percentage of the amount ingested, will be computed and compared between groups.
Exploratory analysis of EED biomarkers in fecal samples
Stool samples will be collected using plastic-lined diapers and aliquots of stool transferred using sterile metal spatulas into cryovials and flash frozen in liquid nitrogen within five minutes of defecation. Novel fecal biomarkers will be sought to assess bowel integrity and levels of inflammation noninvasively. Evaluation of these markers of barrier dysfunction might pave the way for assessment of intestinal mucosal permeability and inflammatory response in diagnosing the degree and progression of EED in vulnerable populations without the need for the relatively lengthy and cumbersome dual-sugar absorption test [53–61]. Markers of interest include cytokines, interleukins, immunoglobulins, REG1A, REG1B, calprotectin, and zonulin.
We also plan to continue our exploration of human mRNA transcripts of putative inflammatory and antimicrobial mediators that are upregulated in EED and found in fecal samples. Nucleic acids are first extracted using a NucliSens EasyMAG kit (BioMérieux), which is a bead-based RNA-specific method. A digital droplet PCR is used to detect low copy numbers of human mRNA reliably . The digital droplet PCR is unique in that after the PCR is set up in the aqueous phase, the reaction is emulsified in oil, creating 20,000 aqueous bubbles that are counted by laser for positive fluorescence of the probe of interest. The quantities of mRNA in a given sample are normalized to a housekeeping gene present in abundant quantities. A number of promising targets that correlate with EED have already been identified (unpublished data) and these will be pursued further in this study, with the aim of identifying one or more that may serve as less cumbersome biomarkers of EED. Newer whole human transcriptome microarray technology [63, 64] will also be employed to interrogate these samples, as another approach to identify these biomarkers.
To investigate correlations between bacterial populations in the gut and EED, as well as the effects of the legume interventions on gut microbe populations, bacterial genomic DNA will be prepared separately from fecal samples obtained from study children. To prepare the stool samples for sequencing, genomic DNA will be extracted using standard protocols (http://hmpdacc.org/resources/tools_protocols.php) V1-V3 variable regions of bacterial 16S rRNA genes will be amplified by PCR and the resulting amplicons will be subjected to high throughput sequencing. We will use the to Illumina Miseq platform for targeted 16S rRNA sequencing. This deep sequencing permits the recognition of rare members of the microbiota that may be shared or that may discriminate these human populations. 16S rRNA sequences will be processed through the pipeline developed by human microbiome projects at The Genome Institute at Washington University. In brief, quality trimming, denosing and chimera removal will be performed to yield high quality reads . Reads passing the above filtering steps will be classified from the phylum to the genus level using an automated implementation of the Ribosomal Database Project’s Classifier . Species-level bacterial phylotypes will be defined as organisms sharing at least 97 % nucleotide sequence identity in the V1-V3 regions of their 16S rRNA genes using the Mothur software package .
Shotgun sequencing will be performed to examine the taxa that are prominently represented in the infant microbiome but inefficiently captured by PCR primers designed to target other variable regions. Whole genome sequencing libraries will be prepared following a standard protocol from Illumina. Ten GB 100 bp pair-end reads per sample will be sequenced on the Illumina HiSeq platform. Reads passing quality filtering and human contamination removal will be classified to species level based on nucleotide sequence alignment to reference genomes using Real Time Genomics map software (Real Time Genomics, San Bruno, CA, USA). Read mapping results will be transformed to values representing the coverage of each reference genome using refcov software (http://gmt.genome.wustl.edu/packages/refcov/index.html). The coverage values are then scaled to a million aligned reads to calculate ‘depth of coverage per million reads’ values. Taxa-based analysis will be the same as the 16S rRNA genes approach described above.
Data management and analysis
Clinical data such as anthropometry, demography, and morbidity data, will be collected on standardized forms by field workers. Field workers will be trained and validated with the questionnaires and measurements they collect prior to data collection. Anthropometric Z-scores will be calculated from the 2006 WHO Multicentre Growth Reference Survey . Data will be double-entered into a password-protected Microsoft Access database by research assistants blinded to food assignments. All discrepancies will be resolved by examination of the original data cards and discussion with the relevant field workers, possibly including repeat examination of the child and questioning of the caregiver as needed. Once all values are entered and discrepancies resolved, the data set will be locked.
Comparisons will be made between cowpea flour and corn–soy blended flour, separately from common beans and corn–soy blend. Fisher’s exact test will generally be used to compare discrete parameters, and Student’s t test will be used for continuous parameters. A difference with P < 0.05 will be considered statistically significant. These statistical methods will also be used for all clinical data and EED measures (e.g., L:M ratio), which are continuous measures of EED status.
The microbiome data analyses and interpretation require specialized tools. Read-based metabolic profiling of the microbial communities is performed by first using whole genome sequencing reads from each sample to probe the Kyoto Encyclopedia of Genes and Genomes using Mblastx (MultiCoreWare, St. Louis, MO) and then search results are run through the metabolic pathway pipeline developed as part of the Human Microbiome Project to obtain enzyme and pathway abundance and coverage from metagenomic communities. Differentially expressed enzymes and pathways between groups (both between intervention groups as well as within the same individual, for example before and after intervention) are identified using LEfSe (http://huttenhower.sph.harvard.edu/lefse). Principal component analysis will be performed to identify cluster patterns based on the gene content and metabolic pathway abundance between the legume and corn–soy blend groups.
All microbiome data will be rarefied to ensure the sample comparisons are at the same read depth. Ordination methods will facilitate the pattern analysis of the high dimensional microbiome data by reducing its dimensionality to 2–3 dimensions, such that the original distance between the samples is preserved to a large degree. In particular, principal component analysis with Bray–Curtis dissimilarity will be used to identify the sample cluster pattern based on the microbial community structures.
A robust statistical approach that is widely used in ecology and metagenomics, Permutational Multivariate Analysis of Variance (perMANOVA in the R software package, http://www.r-project.org), will be used to test the significance of differences at the whole bacterial community level between two or more groups. Dissimilarity matrices will be generated based on the taxon abundance table, and will be treated as the dependent variable in the model. We will use metastats, a program developed in the Human Microbiome Project, to detect differentially abundant taxa between groups . The statistical tests will be corrected for false discovery rates using the q-values approach, an approach that has been effectively applied in metagenomic studies for identifying the taxa of interest. Indices such as richness (the total different number of taxa) and Shannon diversity (the number of taxa present as well as the relative abundances) that categorize the complexity of the bacteria community will be computed. Student’s t-test will be used to compare the diversity differences between groups.
Childhood undernutrition remains a formidable challenge in global health, contributing greatly to the continued unacceptably high rates of mortality among young children, and even more so to decreased achievement of full adult cognitive and physical growth potential . No significant reduction in the overall damage caused by undernutrition is likely to be successful without making major inroads against EED and stunting [4, 71], especially since the two have a synergistic detrimental effect [72, 73]. We have presented here the outlines of two randomized investigator-blinded prospective controlled clinical trials to test the hypothesis that dietary supplementation with legumes at times of peak vulnerability in child growth and development will decrease EED and stunting.
Prior studies using pharmacological interventions to reduce EED have only shown relatively modest and transient improvements in gut health and it remains unclear whether any such intervention targeting inflammation will be effective in reducing EED . Indeed it is quite possible that sanitation and hygiene interventions may be the most effective interventions to ameliorate EED and stunting [5, 75], but the extremely high cost and logistical challenges of implementing such interventions throughout the developing world unfortunately remain exceedingly high.
The trials presented here are novel in that we have taken a food-based approach to targeting EED , carefully selecting two legumes – common beans and cowpeas – that have high levels of both macronutrients and micronutrients that make them appealing choices as complementary foods. We also postulate that their anti-inflammatory effects in the gut may also help to decrease the chronic inflammatory state that accompanies EED  and stunting .
Beans have been cultivated for more than 700 years and were harvested in Africa long before the colonial era. They might thus serve as major sources of protein in southeast Africa if proven to be of benefit in these young children. Indeed, the Great Lakes region of Africa has the highest per capita bean consumption in the world, demonstrating the cultural acceptability of such legumes in our target population . However, the consumption of legumes among young children in rural Malawi remains extremely low due to the preference for maize and other carbohydrate-rich staples . Legumes could therefore serve as a complementary food in this high-risk population, with key measurable endpoints and biomarkers, including markers of EED and growth parameters.
One limitation of the study is the lack of complete blinding of the assigned flour interventions between the three groups. Although the different flours will be packaged similarly and, at first pass, have very similar appearances and textures, a curious caregiver could ultimately determine the nature of the intervention by taste and/or smell, and subsequently trade their assigned intervention with another participant from the same village. We are optimistic that this will be a relatively unlikely occurrence, as our past research into this possibility in a very similar population did not demonstrate any evidence of sharing or trading either assigned food  or pharmacological  interventions. Extensive education of caregivers of trial participants (virtually all of whom will be the household matriarch) and community engagement with village leaders about the need to comply with the assigned interventions should also decrease this possibility. Our preliminary data from acceptability studies did not demonstrate that the children themselves did not like the flours, and thus they will not likely be the motivation for any trading either.
The possibility of sharing of the flours with other members in the household also exists, but this should be relatively even across the three different intervention arms, and relatively unlikely since the amount of flour to be consumed each day is rather small and unlikely to be particularly satisfying to a hungry family member.
As the trials get underway, we may also use this robust study design and multi-site enrollment of two unique age groups with a presumed range of pathology to consider the addition of sub-studies to explore concepts such as household food consumption, cognitive and physical development, serum or other functional biomarkers of nutritional status, and other health variables such as acute infectious symptoms.
Recruitment of trial participants began in July 2015. Interaction with study subjects is expected to last approximately 18 months.
environmental enteric dysfunction
high-performance liquid chromatography
lactulose to mannitol ratio
polymerase chain reaction
Black RE, Victora CG, Walker SP, Bhutta ZA, Christian P, de Onis M, et al. Maternal and child undernutrition and overweight in low-income and middle-income countries. Lancet. 2013;382(9890):427–51.
Keusch GT, Denno DM, Black RE, Duggan C, Guerrant RL, Lavery JV, et al. Environmental enteric dysfunction: pathogenesis, diagnosis, and clinical consequences. Clin Infect Dis. 2014;59 Suppl 4:S207–12.
Crane RJ, Jones KDJ, Berkley JA. Environmental enteric dysfunction – an overview. CMAM Forum Technical Brief. Community-based Management of Acute Malnutrition Furum; 2014. www.cmamforum.org/Pool/Resources/Environmental-Enteric-Dysfunction-CMAM-Forum-techn-brief-Aug-2014.pdf.
Korpe PS, Petri Jr WA. Environmental enteropathy: critical implications of a poorly understood condition. Trends Mol Med. 2012;18(6):328–36.
Humphrey JH. Child undernutrition, tropical enteropathy, toilets, and handwashing. Lancet. 2009;374(9694):1032–5.
Prendergast A, Kelly P. Enteropathies in the developing world: neglected effects on global health. Am J Trop Med Hyg. 2012;86(5):756–63.
Ruel MT, Alderman H. Nutrition-sensitive interventions and programmes: how can they help to accelerate progress in improving maternal and child nutrition? Lancet. 2013;382(9891):536–51.
Ryan KN, Stephenson KB, Trehan I, Shulman RJ, Thakwalakwa C, Murray E, et al. Zinc or albendazole attenuates the progression of environmental enteropathy: a randomized controlled trial. Clin Gastroenterol Hepatol. 2014;12(9):1507–13.
Siddiq M, Uebersax MA. Dry beans and pulses: production and consumption – an overview. In: Siddiq M, Uebersax MA, editors. Dry beans and pulses: production, processing and nutrition. Ames, Iowa: Wiley Blackwell; 2012. p. 3–22.
Mensa-Wilmot Y, Phillips RD, Sefa-Dedeh S. Acceptability of extrusion cooked cereal/legume weaning food supplements to Ghanaian mothers. Int J Food Sci Nutr. 2001;52(1):83–90.
McKay S, Gaudier E, Campbell DI, Prentice AM, Albers R. Environmental enteropathy: new targets for nutritional interventions. Int Health. 2010;2(3):172–80.
Galpin L, Manary MJ, Fleming K, Ou CN, Ashorn P, Shulman RJ. Effect of Lactobacillus GG on intestinal integrity in Malawian children at risk of tropical enteropathy. Am J Clin Nutr. 2005;82(5):1040–5.
Trehan I, Shulman RJ, Ou CN, Maleta K, Manary MJ. A randomized, double-blind, placebo-controlled trial of rifaximin, a nonabsorbable antibiotic, in the treatment of tropical enteropathy. Am J Gastroenterol. 2009;104(9):2326–33.
Esmaillzadeh A, Azadbakht L. Legume consumption is inversely associated with serum concentrations of adhesion molecules and inflammatory biomarkers among Iranian women. J Nutr. 2012;142(2):334–9.
Sandoval M, Ronzio RA, Muanza DN, Clark DA, Miller MJ. Peroxynitrite-induced apoptosis in epithelial (T84) and macrophage (RAW 264.7) cell lines: effect of legume-derived polyphenols (phytolens). Nitric Oxide. 1997;1(6):476–83.
Zhang Z, Lanza E, Kris-Etherton PM, Colburn NH, Bagshaw D, Rovine MJ, et al. A high legume low glycemic index diet improves serum lipid profiles in men. Lipids. 2010;45(9):765–75.
Zhang Z, Lanza E, Ross AC, Albert PS, Colburn NH, Rovine MJ, et al. A high-legume low-glycemic index diet reduces fasting plasma leptin in middle-aged insulin-resistant and -sensitive men. Eur J Clin Nutr. 2011;65(3):415–8.
Messina V. Nutritional and health benefits of dried beans. Am J Clin Nutr. 2014;100 Suppl 1:437S–42.
Hayde VC, Ramon GG, Lorenzo GO, Dave OB, Rosalia RC, Paul W, et al. Non-digestible fraction of beans (Phaseolus vulgaris L.) modulates signalling pathway genes at an early stage of colon cancer in Sprague–Dawley rats. Br J Nutr. 2012;108 Suppl 1:S145–54.
Campos-Vega R, Garcia-Gasca T, Guevara-Gonzalez R, Ramos-Gomez M, Oomah BD, Loarca-Pina G. Human gut flora-fermented nondigestible fraction from cooked bean (Phaseolus vulgaris L.) modifies protein expression associated with apoptosis, cell cycle arrest, and proliferation in human adenocarcinoma colon cancer cells. J Agric Food Chem. 2012;60(51):12443–50.
Vergara-Castaneda HA, Guevara-Gonzalez RG, Ramos-Gomez M, Reynoso-Camacho R, Guzman-Maldonado H, Feregrino-Perez AA, et al. Non-digestible fraction of cooked bean (Phaseolus vulgaris L.) cultivar Bayo Madero suppresses colonic aberrant crypt foci in azoxymethane-induced rats. Food Funct. 2010;1(3):294–300.
Feregrino-Perez AA, Berumen LC, Garcia-Alcocer G, Guevara-Gonzalez RG, Ramos-Gomez M, Reynoso-Camacho R, et al. Composition and chemopreventive effect of polysaccharides from common beans (Phaseolus vulgaris L.) on azoxymethane-induced colon cancer. J Agric Food Chem. 2008;56(18):8737–44.
Ojwang LO, Banerjee N, Noratto GD, Angel-Morales G, Hachibamba T, Awika JM, et al. Polyphenolic extracts from cowpea (Vigna unguiculata) protect colonic myofibroblasts (CCD18Co cells) from lipopolysaccharide (LPS)-induced inflammation–modulation of microRNA 126. Food Funct. 2015;6(1):146–54.
Hachibamba T, Dykes L, Awika J, Minnaar A, Duodu KG. Effect of simulated gastrointestinal digestion on phenolic composition and antioxidant capacity of cooked cowpea (Vigna unguiculata) varieties. Int J Food Sci Tech. 2013;48(12):2638–49.
Kau AL, Ahern PP, Griffin NW, Goodman AL, Gordon JI. Human nutrition, the gut microbiome and the immune system. Nature. 2011;474(7351):327–36.
Bakhtiar SM, LeBlanc JG, Salvucci E, Ali A, Martin R, Langella P, et al. Implications of the human microbiome in inflammatory bowel diseases. FEMS Microbiol Lett. 2013;342(1):10–7.
Leone V, Chang EB, Devkota S. Diet, microbes, and host genetics: the perfect storm in inflammatory bowel diseases. J Gastroenterol. 2013;48(3):315–21.
Trehan I, Goldbach HS, LaGrone LN, Meuli GJ, Wang RJ, Maleta KM, et al. Antibiotics as part of the management of severe acute malnutrition. N Engl J Med. 2013;368(5):425–35.
Smith MI, Yatsunenko T, Manary MJ, Trehan I, Mkakosya R, Cheng J, et al. Gut microbiomes of Malawian twin pairs discordant for kwashiorkor. Science. 2013;339(6119):548–54.
Gordon JI, Dewey KG, Mills DA, Medzhitov RM. The human gut microbiota and undernutrition. Sci Transl Med. 2012;4(137):137ps12.
De Filippo C, Cavalieri D, Di Paola M, Ramazzotti M, Poullet JB, Massart S, et al. Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa. Proc Natl Acad Sci U S A. 2010;107(33):14691–6.
Smith HE, Ryan KN, Stephenson KB, Westcott C, Thakwalakwa C, Maleta K, et al. Multiple micronutrient supplementation transiently ameliorates environmental enteropathy in Malawian children aged 12–35 months in a randomized controlled clinical trial. J Nutr. 2014;144(12):2059–65.
World Medical Association. World Medical Association Declaration of Helsinki: ethical principles for medical research involving human subjects. JAMA. 2013;310(20):2191–4.
Denno DM, VanBuskirk K, Nelson ZC, Musser CA, Hay Burgess DC, Tarr PI. Use of the lactulose to mannitol ratio to evaluate childhood environmental enteric dysfunction: a systematic review. Clin Infect Dis. 2014;59 Suppl 4:S213–9.
Faul F, Erdfelder E, Lang AG, Buchner A. G*Power 3: a flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behav Res Methods. 2007;39(2):175–91.
PAHO. Guiding principles for complementary feeding of the breastfed child. Washington DC: Pan American Health Organization; 2003.
WHO. Feeding the non-breastfed child 6–24 months of age. Geneva: World Health Organization; 2004.
USDA. Dietary guidelines for Americans. Washington: United States Department of Agriculture Center for Nutrition Policy and Promotion; 2010.
Akibode CS. Trends in the production, trade, and consumption of food-legume crops in sub-Saharan Africa. East Lansing, MI: Michigan State University; 2011.
Al-Kahldi S. Phytohaemagglutinin (kidney bean lectin). In: Lampel KA, Al-Khaldi S, Cahill SM, editors. Bad bug book: foodborne pathogenic microorganisms and natural toxins. 2nd ed. Silver Spring, MD: Food and Drug Administration; 2012.
Henningsson AM, Nyman EM, Bjorck IM. Content of short-chain fatty acids in the hindgut of rats fed processed bean (Phaseolus vulgaris) flours varying in distribution and content of indigestible carbohydrates. Br J Nutr. 2001;86(3):379–89.
Campos-Vega R, Oomah B, Loarca-Piña G, Vergara-Castañeda H. Common beans and their non-digestible fraction: cancer inhibitory activity—an overview. Foods. 2013;2(3):374.
Arnold BF, Galiani S, Ram PK, Hubbard AE, Briceno B, Gertler PJ, et al. Optimal recall period for caregiver-reported illness in risk factor and intervention studies: a multicountry study. Am J Epidemiol. 2013;177(4):361–70.
WHO. WHO child growth standards and the identification of severe acute malnutrition in infants and children. Geneva: World Health Organization, United Nations Children’s Fund; 2009.
Trehan I, Manary MJ. Management of severe acute malnutrition in low-income and middle-income countries. Arch Dis Child. 2015;100(3):283–7.
Ukabam SO, Cooper BT. Small intestinal permeability as an indicator of jejunal mucosal recovery in patients with celiac sprue on a gluten-free diet. J Clin Gastroenterol. 1985;7(3):232–6.
Zhang Y, Lee B, Thompson M, Glass R, Cama RI, Figueroa D, et al. Lactulose-mannitol intestinal permeability test in children with diarrhea caused by rotavirus and cryptosporidium. Diarrhea Working Group, Peru. J Pediatr Gastroenterol Nutr. 2000;31(1):16–21.
Chen P, Soares AM, Lima AA, Gamble MV, Schorling JB, Conway M, et al. Association of vitamin A and zinc status with altered intestinal permeability: analyses of cohort data from northeastern Brazil. J Health Popul Nutr. 2003;21(4):309–15.
Goto K, Chew F, Torun B, Peerson JM, Brown KH. Epidemiology of altered intestinal permeability to lactulose and mannitol in Guatemalan infants. J Pediatr Gastroenterol Nutr. 1999;28(3):282–90.
Lunn PG, Northrop-Clewes CA, Downes RM. Intestinal permeability, mucosal injury, and growth faltering in Gambian infants. Lancet. 1991;338(8772):907–10.
Shulman RJ, Eakin MN, Czyzewski DI, Jarrett M, Ou CN. Increased gastrointestinal permeability and gut inflammation in children with functional abdominal pain and irritable bowel syndrome. J Pediatr. 2008;153(5):646–50.
Lee GO, Kosek P, Lima AA, Singh R, Yori PP, Olortegui MP, et al. Lactulose:mannitol diagnostic test by HPLC and LC-MSMS platforms: considerations for field studies of intestinal barrier function and environmental enteropathy. J Pediatr Gastroenterol Nutr. 2014;59(4):544–50.
Derikx JP, Luyer MD, Heineman E, Buurman WA. Non-invasive markers of gut wall integrity in health and disease. World J Gastroenterol. 2010;16(42):5272–9.
Dieckgraefe BK, Crimmins DL, Landt V, Houchen C, Anant S, Porche-Sorbet R, et al. Expression of the regenerating gene family in inflammatory bowel disease mucosa: Reg Iα upregulation, processing, and antiapoptotic activity. J Investig Med. 2002;50(6):421–34.
Sekikawa A, Fukui H, Fujii S, Nanakin A, Kanda N, Uenoyama Y, et al. Possible role of REG Iα protein in ulcerative colitis and colitic cancer. Gut. 2005;54(10):1437–44.
Sekikawa A, Fukui H, Fujii S, Takeda J, Nanakin A, Hisatsune H, et al. REG Iα protein may function as a trophic and/or anti-apoptotic factor in the development of gastric cancer. Gastroenterology. 2005;128(3):642–53.
Fasano A. Zonulin and its regulation of intestinal barrier function: the biological door to inflammation, autoimmunity, and cancer. Physiol Rev. 2011;91(1):151–75.
Hovhannisyan Z, Treatman J, Littman DR, Mayer L. Characterization of interleukin-17-producing regulatory T cells in inflamed intestinal mucosa from patients with inflammatory bowel diseases. Gastroenterology. 2011;140(3):957–65.
Lammers KM, Khandelwal S, Chaudhry F, Kryszak D, Puppa EL, Casolaro V, et al. Identification of a novel immunomodulatory gliadin peptide that causes interleukin-8 release in a chemokine receptor CXCR3-dependent manner only in patients with coeliac disease. Immunology. 2011;132(3):432–40.
Hestvik E, Tumwine JK, Tylleskar T, Grahnquist L, Ndeezi G, Kaddu-Mulindwa DH, et al. Faecal calprotectin concentrations in apparently healthy children aged 0–12 years in urban Kampala, Uganda: a community-based survey. BMC Pediatr. 2011;11:9.
Judd TA, Day AS, Lemberg DA, Turner D, Leach ST. Update of fecal markers of inflammation in inflammatory bowel disease. J Gastroenterol Hepatol. 2011;26(10):1493–9.
Agapova S, Stephenson K, Manary M, Weisz A, Tarr PI, Mkakosya R, et al. Detection of low-concentration host mRNA transcripts in Malawian children at risk for environmental enteropathy. J Pediatr Gastroenterol Nutr. 2013;56(1):66–71.
Loens K, Bergs K, Ursi D, Goossens H, Ieven M. Evaluation of NucliSens easyMAG for automated nucleic acid extraction from various clinical specimens. J Clin Microbiol. 2007;45(2):421–5.
Dundas N, Leos NK, Mitui M, Revell P, Rogers BB. Comparison of automated nucleic acid extraction methods with manual extraction. J Mol Diagn. 2008;10(4):311–6.
Zhou Y, Gao H, Mihindukulasuriya KA, La Rosa PS, Wylie KM, Vishnivetskaya T, et al. Biogeography of the ecosystems of the healthy human body. Genome Biol. 2013;14(1):R1.
Wang Q, Garrity GM, Tiedje JM, Cole JR. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol. 2007;73(16):5261–7.
Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, et al. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol. 2009;75(23):7537–41.
WHO Multicentre Growth Reference Study Group. Assessment of differences in linear growth among populations in the WHO Multicentre Growth Reference Study. Acta Paediatr Suppl. 2006;450:56–65.
White JR, Nagarajan N, Pop M. Statistical methods for detecting differentially abundant features in clinical metagenomic samples. PLoS Comput Biol. 2009;5(4):e1000352.
Guerrant RL, Deboer MD, Moore SR, Scharf RJ, Lima AA. The impoverished gut-a triple burden of diarrhoea, stunting and chronic disease. Nat Rev Gastroenterol Hepatol. 2012;10(4):220–9.
Keusch GT, Rosenberg IH, Denno DM, Duggan C, Guerrant RL, Lavery JV, et al. Implications of acquired environmental enteric dysfunction for growth and stunting in infants and children living in low- and middle-income countries. Food Nutr Bull. 2013;34(3):357–64.
Campbell DI, Elia M, Lunn PG. Growth faltering in rural Gambian infants is associated with impaired small intestinal barrier function, leading to endotoxemia and systemic inflammation. J Nutr. 2003;133(5):1332–8.
Kosek M, Haque R, Lima A, Babji S, Shrestha S, Qureshi S, et al. Fecal markers of intestinal inflammation and permeability associated with the subsequent acquisition of linear growth deficits in infants. Am J Trop Med Hyg. 2013;88(2):390–6.
Petri WA, Naylor C, Haque R. Environmental enteropathy and malnutrition: do we know enough to intervene? BMC Med. 2014;12(1):187.
Ngure FM, Reid BM, Humphrey JH, Mbuya MN, Pelto G, Stoltzfus RJ. Water, sanitation, and hygiene (WASH), environmental enteropathy, nutrition, and early child development: making the links. Ann N Y Acad Sci. 2014;1308(1):118–28.
Prendergast AJ, Rukobo S, Chasekwa B, Mutasa K, Ntozini R, Mbuya MN, et al. Stunting is characterized by chronic inflammation in Zimbabwean infants. PLoS One. 2014;9(2):e86928.
Jackson J, Kinabo J, Mamiro P, Mamiro D, Jideani V. Utilization of Dry Beans and Pulses in Africa. In: Siddiq M, Uebersax MA, editors. Dry Beans and Pulses: Production, Processing and Nutrition. Ames, Iowa: Wiley-Blackwell; 2012. p. 261–82.
Lin CA, Boslaugh S, Ciliberto HM, Maleta K, Ashorn P, Briend A, et al. A prospective assessment of food and nutrient intake in a population of Malawian children at risk for kwashiorkor. J Pediatr Gastroenterol Nutr. 2007;44(4):487–93.
Wang RJ, Trehan I, LaGrone LN, Weisz AJ, Thakwalakwa CM, Maleta KM, et al. Investigation of food acceptability and feeding practices for lipid nutrient supplements and blended flours used to treat moderate malnutrition. J Nutr Educ Behav. 2013;45(3):258–63.
Study funding is provided by the Feed the Future Innovation Lab for Collaborative Research on Grain Legumes, supported by the United States Agency for International Development; and the Children’s Discovery Institute of Washington University and St. Louis Children’s Hospital. Study funders played no role in the design or conduct of the study or in future data analyses and publication decisions.
The authors declare that they have no competing interests.
IT wrote the first draft of the manuscript and will provide direct field supervision of the trial. IT, NSB, AZW, LBB, KBS, and SEA prepared the field research sites and supervised flour production for the trial. IT, LBB, KBS, and SEA are enrolling subjects, conducting clinical assessments, and collecting urine and stool specimens. NSB, TNN, and UKC prepared the legume flours and conducted acceptability testing of the legumes. IT, KMM, and MJM designed the trial. KMM and MJM will provide overall administrative support to the trial. All authors read and approved the final manuscript.
About this article
- common beans
- complementary foods
- dual-sugar absorption test
- environmental enteric dysfunction
- environmental enteropathy