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A randomized comparative study of patients undergoing myocardial revascularization with or without cardiopulmonary bypass surgery: The MASS III Trial
Trials volume 9, Article number: 52 (2008)
The MASS III Trial is a large project from a single institution, The Heart Institute of the University of Sao Paulo, Brazil (InCor), enrolling patients with coronary artery disease and preserved ventricular function. The aim of the MASS III Trial is to compare medical effectiveness, cerebral injury, quality of life, and the cost-effectiveness of coronary surgery with and without of cardiopulmonary bypass in patients with multivessel coronary disease referred for both strategies. The primary endpoint should be a composite of cardiovascular mortality, cerebrovascular accident, nonfatal myocardial infarction, and refractory angina requiring revascularization. The secondary end points in this trial include noncardiac mortality, presence and severity of angina, quality of life based on the SF-36 Questionnaire, and cost-effectiveness at discharge and at 5-year follow-up. In this scenario, we will analyze the cost of the initial procedure, hospital length of stay, resource utilization, repeat hospitalization, and repeat revascularization events during the follow-up. Exercise capacity will be assessed at 6-months, 12-months, and the end of follow-up. A neurocognitive evaluation will be assessed in a subset of subjects using the Brain Resource Center computerized neurocognitive battery. Furthermore, magnetic resonance imaging will be made to detect any cerebral injury before and after procedures in patients who undergo coronary artery surgery with and without cardiopulmonary bypass.
Clinical Trial registration information
ISRCTN59539154 Off-pump vs. on-pump surgery in patients with Stable CAD MASS III
Coronary bypass surgery performed without the use of cardiopulmonary bypass (off-pump surgery) has been used sporadically since the beginning of the bypass surgery era in 1967, but the use of this strategy increased substantially during the 1990s. The major reason for the increased use of off-pump surgery was the hope that this strategy would decrease perioperative morbidity and possibly mortality by eliminating cardiopulmonary bypass (on-pump surgery). The fear concerning off-pump surgery has been that the difficulty of operating with the heart beating would lead to less-complete and less-effective revascularization at the time of surgery and worse long-term outcomes. These advantages and disadvantages have been examined in several studies that compared the outcomes of patients undergoing off-pump and on-pump surgery [1–4]. Observational trials often have shown bigger differences in short-term complications, usually in favor of off-pump surgery, but analyses of these trials are complicated by patient selection. On the other hand, randomized trials usually have shown small differences in perioperative outcomes, usually slightly in favor of off-pump surgery, mostly including low-risk patients.
Long-term follow-up studies, both randomized and observational, have sometimes noted inferior outcomes after off-pump compared with on-pump surgery, manifested as decreased patency, increased risk of repeat revascularization, or increased mortality. Yet, other studies have shown no or few long-term differences that usually have been attributed to a lack of experience with off-pump surgery [3, 4].
The absence of guidelines for the use of one or the other technique has allowed individual decision-making according to the experience of the surgeon . The rationality for off-pump surgery is reduced morbidity, and reduced adverse effects attributed to on-pump surgery, including an inflammatory response caused by the circulation of blood through the cardiopulmonary circuit and the formation of microemboli . In this context, the advantages and disadvantages of both strategies have been considered, and critical appraisal is made of the evidence available. The validity of evidence has been assessed using different criteria, including study design, size of surgical populations, and the quality of statistical analyses .
The aim of the MASS III Trial is to compare medical effectiveness, safety, cerebral injury, quality of life, cost-effectiveness of coronary surgery with and without of cardiopulmonary bypass in on-pump and off-pump techniques in patients referred for both strategies. The primary endpoint should be a composite of cardiovascular mortality, cerebrovascular accident, nonfatal myocardial infarction, and refractory angina requiring revascularization.
Coronary artery surgery with and without cardiopulmonary bypass plays an important role in the treatment and management of ischemic heart disease. Both treatment strategies have their own advantages and disadvantages. On-pump strategies provide more complete revascularization and require fewer repeat interventions compared with off-pump surgery. However, the procedure is more invasive and is associated with cardiac as well as noncardiac surgery morbidity [8, 9]. Major neurological complications after conventional cardiac surgery have been reported to occur in 3.1% of patients . Additionally, neuropsychological dysfunction is increasingly being recognized as a complication of on-pump surgery. Cognitive deficits can be documented accurately  and may occur in up to 38% of patients . The increasing awareness of the cerebral complications following on-pump surgery especially in the elderly  has led to a renewed interest in coronary surgery on the beating heart. The Utrecht Octopus method is a technique developed to avoid cardiopulmonary bypass and the complications associated with its use .
The role of off-pump surgery, however, is the subject of debate. Opponents emphasize the excellent results of conventional on-pump surgery and express their concerns about the safety, early anastomotic failure, and eventual incomplete revascularization related to off-pump strategies .
Moreover, although widely accepted, the suspected deleterious role of cardiopulmonary circulation in the genesis of adverse cerebral outcomes has not been completely proved, simply because an appropriate control group has never been available. A limited number of randomized clinical trials have directly compared coronary surgery with and without cardiopulmonary bypass circulation [16, 17].
The MASS III trial is a single-center, prospective, randomized clinical trial, of which the design, timing of investigation and definitions of main outcome events are presented in Figure 1, Table 1 and Table 2, respectively. The MASS III Trial provided external blinded committee. So, all nonfatal clinical events, including MI, Stroke, refractory angina requiring revascularization, will undergo central adjudication by independent Clinical Events Committee (CEC). The role of CEC will be to insure that all primary endpoint are adjudicated uniformly. Furthermore a cardiopulmonary surgeon, a cardiologist and a neurologist formed the clinical event committee and confirm and classify the major adverse cardiac and cerebrovascular events (MACCE), blinded to the treatment. To verify whether important differences in the incidence of MACCE exist between the treatment groups, the Data Monitoring Committee performed an interim analysis after the first patients 100 had entered each arm of study. The three members of this committee are experienced in patient-oriented research, are independent of the study, and may also offer unsolicited recommendations. The sample size calculations are based on the assumptions that the actuarial freedom from cardiac event rate 5 years after on-pump surgery is 95% and that off-pump surgery did not decrease the rate by more than 10%. The α error is set at 0.05, and the β error is set at 0.20. The required sample size is 153 in each group for a total of 306 patients.
Primary Composite End Points
The primary endpoint should be a composite of cardiovascular mortality, cerebrovascular accident, nonfatal myocardial infarction, and refractory angina requiring revascularization across 5 years of follow-up.
It is expected that the need for repeat revascularization after coronary surgery with or without cardiopulmonary bypass will be similar. As mentioned, a controversy discussed in the literature indicates that coronary surgery with or without cardiopulmonary bypass circulation has equal effectiveness in terms of myocardial revascularization. Therefore, repeat interventions can be a major clinical event. Therefore repeat interventions will be considered as early failure of treatment in this study. In addition, the use of cardiopulmonary bypass can be associated with considerable cerebral injury, in particular neuropsychological deficits [11, 12]. So, the primary end point in the comparison of surgery with and without cardiopulmonary bypass is also a cerebral event. This is defined as the proportion of patients free of the combined event of fatal and nonfatal cardiovascular accidents and cognitive dysfunction, whichever occurs first, to be determined in-hospital and during the 5 years of follow-up.
Secondary End Points
The secondary end points in this trial include non-cardiac mortality, presence and severity of angina, quality of life using the SF-36 Questionnaire , and cost-effectiveness at discharge and at 5-year follow-up. In this scenario, we will analyze the cost of the initial procedure, hospital length of stay, resource utilization, repeat hospitalization, and repeat revascularization events during the follow-up. Exercise capacity will be assessed at 6-month, 12-month, and end follow-up.
In the MASS III trial, no difference is expected in cardiac outcome; nevertheless, cardiac death, myocardial infarction, and repeat revascularization procedures will be assessed in-hospital and at 1, 6, and 12 months and yearly thereafter. Cognitive function will also be assessed at baseline and at 1, 6, and 12 months. It is expected that most patients will show a decline in this early postoperative period. However, the rapid recovery and short hospital stay of the nonselected patients who undergo coronary surgery in our hospital without extracorporeal circulation suggest that the benefits of this strategy may be especially reflected in a reduction of neuropsychological injury in the early postoperative period.
Patients with angiographically documented proximal multivessel coronary stenosis of more than 70% by visual assessment, stable angina, and preserved ventricular function were considered for inclusion in this study. Furthermore, they were eligible if they were referred for isolated coronary bypass surgery for the first time and the off-pump procedure was deemed technically feasible. Patients were enrolled and randomized if surgeons agreed that revascularization could be attained by either strategy. This selection predominantly depends on the precise location of the stenoses, the anticipated capacity of the heart to endure temporary occlusion of the involved coronary arteries, and hemodynamic consequences of local immobilization of the ventricular wall. In these conditions, bypass grafting of posterior coronary arteries may result in a significant drop in left ventricular stroke volume upon presentation of these vessels .
All angiograms were reviewed and a surgical plan was documented before randomization. The selection criteria for the MASS III trial are depicted in Table 3.
Trial operators were required to perform optimum coronary revascularization in accordance with current best practices. The surgery was performed by physicians experienced in both on-pump and off-pump bypass surgery. Surgical access to the heart was through a standard median sternotomy in all cases. All incisions and closure techniques were the same for groups, limiting variability and maintaining blinding of group assignment for patients, family, and referring cardiologists. A cell saver reservoir (COBE Cardiovascular, Inc. Arvada, CO) was spun down and returned to all patients when the quantity was sufficient.
Off-pump surgery used the Octopus stabilizer described in detail elsewhere . In brief, the distal ends of the 2 suction arms of the stabilizer are placed on the beating heart on both sides of the target coronary artery. The proximal parts are fixed to the operating table. Through the application of negative pressure, the target area of the heart is sufficiently immobilized to allow the safe construction of the anastomosis of the graft with the recipient artery.
Conventional coronary artery surgery with cardiopulmonary bypass was accomplished with every effort made to minimize the impact of cardiopulmonary bypass. Patients without diabetes received 1 gram of hydrocortisone sodium succinate (SoluCortef, Pharmacia & Upjohn Co., New York, NY) intravenously before of anesthesia. This procedure will be made only in the on-pump technique. The patients were routinely cooled to 34° for operations with 3 grafts and 32° for 4 grafts or more. Cold-blood cardioplegia was accomplished with anterograde delivery through the aortic root and retrograde delivery through the coronary sinus. A heparinization protocol of 300 U per kilogram for on-pump surgery and half-dose heparin for off-pump surgery was followed. Protamine was used to reverse the effects of heparinization only in the on-pump patients. All anastomoses were sutured by hand. In the off-pump patients, intracoronary shunts were not used routinely; indications for use included poor visibility, ST-segment changes, and homodynamic instability.
Quality of Life and Cost-effectiveness
Health-related quality of life and treatment costs will be assessed to evaluate the relative cost-effectiveness for the MASS III trial population. Heath-related quality of life and functional status will be assessed using a combination of generic and disease-specific measures selected to cover a broad range of potential health domains that may be affected by coronary artery disease, its treatments, and complications. The SF-36 Questionnaire will be used to assess patients' utilities . Utilities are a global rating of health that reflects a patient's preference for his current health status relative to perfect health and are particularly important outcome measures for cost-effectiveness analysis . Medical care resource utilization and cost data will be collected prospectively for the index hospitalization up to discharge and 5-year follow-up for all patients including all costs associated with the index procedure. For each index revascularization procedure, detailed resource utilization will be collected using a standardized case report form. Follow-up medical resource utilization (including hospitalization, out-patient services, and cardiovascular medications) will be assessed by detailed questionnaires that will be completed during each scheduled patient contact.
In evaluation of CABG patients with multivessel disease, neurocognitive status is a crucial outcome variable, along with major adverse cardiac and cerebrovascular event. A subset of subjects will be assessed using the Brain Resource Center  computerized neurocognitive battery. The battery is relatively short and easy to administer and score. The computerized battery measures 5 basic functions: memory, mental and psychomotor speed attention, verbal fluency, and cognitive flexibility.
Statistical Analysis Plan
Patients eligible for the MASS III trial are invited to the outpatient clinic to receive additional information. Candidates for the trial admitted to a referral hospital are visited by one of the trial monitors. After giving consent, patients are randomized. To ensure a reasonable balance, assignment is performed according to a computer-generated list of random permuted blocks that are unknown by the investigators. After randomization, patients are scheduled for the allotted treatment.
The goal of the main analysis is to compare the primary outcome events in this trial. Kaplan Meyer curves will be used for graphic comparison. All values are expressed as mean ± standard deviation or percentage. The continuous variables are compared by the Wilcox rank sum test, whereas the discrete variables are analyzed with Fisher's exact test. The occurrence of outcome events will be compared by means of Cox's proportional hazards model yielding a hazard ratio. The primary data analysis will be based on the intention-to-treat principle. Interim analyses will be carried out annually to evaluate the safety.
For both treatment strategies, the cost of diagnostic procedures, treatment procedures, complications, and short- and long-term differences in effects will be estimated. Marginal costs in monetary terms will be calculated by multiplying unit costs and marginal medical consumption as recorded for each patient.
The MASS III Trial is designed to include patients who need coronary revascularization for relieving angina and better exercise tolerance. Off-pump surgery is a well-established alternative to on-pump surgery in terms of short and mid-term outcomes. However, the benefit of off-pump surgery may depend on the clinical status of patients and the majority of studies were carried out on low-risk surgical patients. Recently, patients with higher risk profile are increasing and in the context of elderly patients with comorbidities, the off-pump surgery might have an important role.
The lack of statement guidelines regarding this issue leaves the decision of using extracorporeal circulation to individual surgeons, and many of them remain unwilling to adopt off-pump surgery technique. This hesitation is still justified, since a more demanding technically procedure is being suggested instead of a successful, well-studied, and reproducible approach. Yet, if the benefits of off-pump surgery became conclusive, this would have several implications for the future of cardiac surgery regarding to quality of patient care as well as cost-effectiveness of the procedure. The MASS III trial is a great opportunity to address all these issues, and it will add relevant information in this field.
MASS III is conducted in accordance with the principles of the Declaration of Helsinki and with laws and regulations of our country. The Ethics Committee of the Heart Institute of University of Sao Paulo, Brazil approved the study protocol. The attending physician obtained written informed consent from the study participants. The patient is told that he or she will be randomized to surgery with or without extracorporeal circulation.
MASS III is designed to include patients who need coronary artery surgery and for whom 2 surgical strategies are feasible. The results of the study may facilitate selection of the most appropriate strategy for individual patients and foster the appropriate use of available resources.
Appendix: Definition of myocardial infarction
A – Q-Wave infarction
See ECG-criteria new Q-wave infarction
Enzyme elevation as follows:
CK-MB elevation 5 × upper limit of normal
B – Enzymatic/non-Q-wave infarction
Enzyme elevation as follows:
CK-MB elevation 5 × upper limit of normal
C – ECG Criteria New Q-Wave myocardial infarction
New QS in 2 associated leads in the absence of left bundle-branch block (LBBB)/Wolf-Parkinson White Syndrome.
New QS in 2 associated leads, defined as ≥ 0.04 seconds broad and/or Q/R ratio ≥ 1/4
Posterior wall infarction: new Broad R-wave (≥ 0.04 seconds) and tall R/wave (R/S ratio▶1 or R-wave ≥ 0.5 mv) in lead V1 and V2 in the absence of right bundle-branch block (RBBB)/right ventricular hypertrophy (RVH)
New permanent LBBB and enzyme elevation
Reversed R-wave progression precordial: decrement R-wave ≥ 0.2 mv in 2 consecutive precordial leads and enzyme elevation.
Any new Q-wave in lead V2 and V3 and enzyme elevation.
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We would like to thank all members of the MASS III Trial for hard work in putting together all the forces in order to performing this study. This study funded partially by Zerbini Foundation. Medical writing support was provided by Ann Conti Morcos during the preparation of this paper, supported by Zerbini Foundation. Responsibility for opinions conclusions and interpretation of data lies with the authors.
Steering Committee Members
Whady Hueb, Neuza HM Lopes, Sergio A Oliveira, José AF Ramires
Protásio Lemos da Luz, Luiz Antonio M César, Paulo Soares, Aécio Góis, Jorge Chiquie Borges
Felipe S Paulitsch.
Sergio A Oliveira, Luis A Dallan, Alexandre C Hueb, Noedir A Stolf
Eulogio Martinez, Expedito Ribeiro, Pedro Lemos, Marco Perin
Cost-effectiveness and Quality of Life
Priscyla Girardi, Célia Regina Nogueira, Myrthes E Takiuti, Teryo Nakano
Ancillary Studies Committee
Neuza HM Lopes, Alexandre Pereira, Aécio Góis, Cláudio C Castro
Data and Safety Monitoring Board
Eliana Lima, Laura Caringe, Giovana Carrasqueira
The MASS III trial is funded in part by the Zerbini Foundation, Sao Paulo, Brazil.
None of the authors of the MASS III Trial has a financial or any other relation that would pose a conflict of interest.
Each of the authors had substantial contributions either on conception and design or on the drafting of the article and critical revision for this important intellectual content. Specifically, WH is the Principal Investigator for the study described in the manuscript, WH, NHML, BJG, SAO and JAFR participated actively in designing and performing the research. Additionally, SAO, LAD, ACH, and NAS performed all the surgeries procedures. WH and NHML are following all the patients during the follow-up clinical visits. Finally, CCC, FSP, and NHML planed the Ancillaries Studies. All authors participated in drafting and revising the manuscript and all authors read and approved the final manuscript.
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Hueb, W., Lopes, N.H.M., Gersh, B.J. et al. A randomized comparative study of patients undergoing myocardial revascularization with or without cardiopulmonary bypass surgery: The MASS III Trial. Trials 9, 52 (2008). https://doi.org/10.1186/1745-6215-9-52
- Cardiopulmonary Bypass
- Repeat Revascularization
- Refractory Angina
- Clinical Event Committee
- Neurocognitive Battery