- Study protocol
- Open Access
Impact of vitamins A, B, C, D, and E supplementation on improvement and mortality rate in ICU patients with coronavirus-19: a structured summary of a study protocol for a randomized controlled trial
Trials volume 21, Article number: 614 (2020)
This study will evaluate the main hypothesis that supplementation with vitamins A, B, C, D, and E significantly improves the severity and mortality rate in ICU patients with COVID-19.
This study is a randomized, single-blinded, two-arm (1:1 ratio) parallel group clinical trial.
We are conducting this study in patients with COVID-19 admitted to intensive care units at the Imam Khomeini Hospital Complex in Tehran, Iran.
The inclusion criteria are as follows: (1) aged between 20 and 60 years, (2) both male and female patients with COVID-19, (3) clinical or definitive diagnosis (using polymerase chain reaction (PCR) test), (4) patients have not participated in other clinical trials, and (5) no renal or hepatic abnormalities.
The exclusion criteria are as follows: (1) patients with specific and rare viral diseases such as HIV and (2) patients who have been undergoing chemotherapy for the past month.
Intervention and comparator
Duration of intervention: 7 days from randomization
Intervention in the treatment group:
Vitamin A 25,000 IU daily
Vitamin D 600,000 IU once during study
Vitamin E 300 IU twice daily
Vitamin C is taken four times per day
B vitamins are taken as a daily Soluvit [which included thiamine nitrate 3.1 mg, sodium riboflavin phosphate 4.9 mg (corresponding to vitamin B2 3.6 mg), nicotinamide 40 mg, pyridoxine hydrochloride 4.9 mg (corresponding to vitamin B6 4.0 mg), sodium pantothenate 16.5 mg (corresponding to pantothenic acid 15 mg), sodium ascorbate 113 mg (corresponding to vitamin C 100 mg), biotin 60 μg, folic acid 400 μg, and cyanocobalamin 5 μg]
The control group will not receive any supplements or placebo.
All supplements are made in Iran except for Soluvit (from Fresenius Kabi, New Zealand).
Weight, height, and BMI
Severity of pulmonary involvement according to CT scan
Respiratory support (invasive or non-invasive)
Percentage of oxygen saturation (SpO2 level)
Serum levels of WBC, CRP, ESR, IL6, IFN-G, and TNF-α
The patient’s body temperature
The presence or absence of involvement of organs other than the lungs (e.g., heart, liver, kidneys)
Duration of hospitalization
At baseline, eligible patients were randomly assigned to a 1:1 ratio to one of two groups: intervention and control. Block randomization is used based on the gender of patients.
Patients are unaware of being placed in the intervention or control groups after signing consent. All treatment staff will be aware of which group each of the patients is in due to the specific conditions of the ICU and the absence of placebo for the control group.
Numbers to be randomized (sample size)
The researchers plan to include 60 patients in total, with 30 patients in each group.
This is the first version of the protocol which started on April 2, 2020. Recruitment began April 2, 2020, and is expected to be complete by July 4, 2020.
The Iranian Registry of Clinical Trials IRCT20200319046819N1. Registered on April 4, 2020
The full protocol is attached as an additional file, accessible from the Trials website (Additional file 1). In the interest in expediting dissemination of this material, the familiar formatting has been eliminated; this letter serves as a summary of the key elements of the full protocol (Fig. 1, Table 1).
Availability of data and materials
The corresponding and first authors have access to the data in a SPSS file.
We are thankful to Tehran University of Medical Sciences & Health Services for supporting the present research with COVID-19 grant number: 99-1-101-47104.
We are grateful to Tehran University of Medical Sciences & Health Services for supporting the present research with COVID-19 grant number: 99-1-101-47104. Tehran University of Medical Sciences confirmed the study design, data collection, analysis, and interpretation of data and writing of the manuscript that are done honestly by the researchers alone.
Ethics approval and consent to participate
The Ethics Committee of the Medical University of Tehran approved the study on March 31, 2020 (IR.TUMS.VCR.REC.1399.090). The participants will complete a written consent prior to inclusion into the study. Information from all patients will be kept confidential, and all those that do not wish to continue can withdraw from the study at any time.
Consent for publication
The authors declare that there are no competing interests in the present study.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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Cite this article
Beigmohammadi, M.T., Bitarafan, S., Hoseindokht, A. et al. Impact of vitamins A, B, C, D, and E supplementation on improvement and mortality rate in ICU patients with coronavirus-19: a structured summary of a study protocol for a randomized controlled trial. Trials 21, 614 (2020). https://doi.org/10.1186/s13063-020-04547-0
- Randomized controlled trial
- Vitamin B
- Vitamin A
- Vitamin D
- Vitamin E
- Vitamin C
- Mortality rate
- Intensive care unit