- Study protocol
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(1,3)-β-D-glucan-based diagnosis of invasive Candida infection versus culture-based diagnosis in patients with sepsis and with an increased risk of invasive Candida infection (CandiSep): study protocol for a randomized controlled trial
Trials volume 19, Article number: 472 (2018)
The time to diagnosis of invasive Candida infection (ICI) is often too long to initiate timely antifungal therapy in patients with sepsis. Elevated serum (1,3)-β-D-glucan (BDG) concentrations have a high diagnostic sensitivity for detecting ICI. However, the clinical significance of elevated BDG concentrations is unclear in critically ill patients. The goal of this study is to investigate whether measurement of BDG in patients with sepsis and a high risk for ICI can be used to decrease the time to empiric antifungal therapy and thus, increase survival.
This prospective multicenter open randomized controlled trial is being conducted in 19 German intensive care units. All adult patients with severe sepsis or septic shock and an increased risk for ICI are eligible for enrolment. Risk factors are total parenteral nutrition, previous abdominal surgery, previous antimicrobial therapy, and renal replacement therapy. Patients with proven ICI or those already treated with systemic antifungal substances are excluded. Patients are allocated to a BDG or standard care group. The standard care group receives targeted antifungal therapy as necessary. In the BDG group, BDG serum samples are taken after randomization and 24 h later. Antifungal therapy is initiated if BDG is ≥80 pg/ml in at least one sample. We plan to enroll 312 patients. The primary outcome is 28-day mortality. Other outcomes include antifungal-free survival within 28 days after enrolment, time to antifungal therapy, and the diagnostic performance of BDG compared to other laboratory tests for early ICI diagnosis. The statistical analysis will be performed according to the intent-to-treat principle.
Because of the high risk of death, American guidelines recommend empiric antifungal therapy in sepsis patients with a high risk of ICI despite the limited evidence for such a recommendation. In contrast, empiric antifungal therapy is not recommended by European guidelines. BDG may offer a way out of this dilemma since BDG potentially identifies patients in need of early antifungals. However, the evidence for such an approach is inconclusive. This clinical study will generate solid evidence for health-care providers and authors of guidelines for the use of BDG in critically ill patients.
Clinicaltrials.gov, NCT02734550. Registered 12 April 2016.
Invasive Candida infection (ICI) is a rising problem in critically ill patients. ICI incidence has increased in hospitalized patients since the beginning of this millennium [1, 2]. Candida albicans alone was assumed to be involved in 13% of all infections acquired in an intensive care unit (ICU) . In addition, the presence of ICI is associated with a high risk of death with an attributable mortality of up to 49% .
The most frequent clinical representation of ICI is candidemia. The gold standard diagnostic test for candidemia is the detection of Candida spp. in a blood culture. Modern blood culture systems, however, detect less than 60% of all cases of ICI . Furthermore, the detection and identification of Candida spp. with a blood culture usually takes several days. Such a diagnostic and thus therapeutic delay substantially increases mortality [6, 7].
Antifungal therapy and source control are the core elements of treating ICI. Targeted therapy in proven ICI is always the right treatment option. Due to the low diagnostic sensitivity of blood culture analysis and the high risk of death in unrecognized ICI, the addition of an antifungal agent to the empiric antimicrobial therapy for sepsis needs to be discussed. American guidelines strongly recommend empiric antifungal therapy in patients with septic shock and with risk factors for ICI . However, the authors point out that the evidence for this recommendation is low and that the most important combination of risk factors in an individual patient has not been established. Furthermore, widespread use of antifungal agents must be balanced against the cost, the risk of toxicity, and the emergence of resistance . Likewise, the Surviving Sepsis Campaign recommends empiric antifungal therapy in sepsis patients if the risk of ICI is sufficiently high. However, the Surviving Sepsis Campaign does not further specify the risk factors qualifying for antifungal therapy . In contrast, the European guidelines recommend only a targeted antifungal therapy, since the lack of data do not support a recommendation for empiric therapy .
A faster diagnosis of ICI might be facilitated by biomarkers. (1,3)-β-D-glucan (BDG) is a cell wall constituent of Candida spp. and other fungi. The assays for BDG measurement use a modification of the limulus-amebocyte-lysate pathway. BDG activates factor G, which in turn activates a coagulation enzyme resulting in the cleavage of p-nitroaniline from a peptide substrate. The photometrically measured p-nitroaniline release over time is used to determine the BDG concentration . The diagnostic accuracy of the BDG measurement has been examined in a meta-analysis as well as in a large multicenter study. BDG was able to distinguish proven or probable ICIs from no ICIs in several patient populations [12, 13]. However, the statistical heterogeneity of the available studies is large . In addition, falsely increased BDG serum concentrations in the absence of ICI may be induced by several common ICU interventions. For example, concurrent bacteremia, hemodialysis membranes, administrations of blood products, and treatment with albumin or immunoglobulin products can interfere with BDG measurements [11, 14, 15]. If relevant, this type of interference would significantly reduce the clinical usability of BDG assays in critically ill patients. Current guidelines hesitate in giving a general recommendation for initiating antifungal therapy relying on BDG results [8, 9, 16].
The high risk of death caused by a delayed initiation of antifungal therapy could be addressed by a preemptive approach where starting antifungal therapy is based on early diagnostic tests such as polymerase chain reaction (PCR) or BDG. However, clinical studies addressing a preemptive antifungal therapy are not conclusive [17, 18]. Clinical guidelines are reluctant in recommending such an approach as robust data are missing [8,9,10].
We are undertaking this trial since there is strong evidence that early antifungal therapy in critically ill patients with ICI increases survival. However, there is missing evidence that BDG can successfully identify those critically ill patients who may profit from early antifungal therapy. The goal of this trial is to investigate whether the measurement of BDG can decrease the time to empiric antifungal therapy and increase survival in patients with severe sepsis or septic shock and an increased likelihood of ICI.
CandiSep is an investigator-initiated prospective, multicenter, randomized, open, and parallel group study comparing mortality for a BDG-driven antifungal therapy versus standard care during 28 days after enrolment in adult patients with severe sepsis or septic shock and with a high risk for ICI. The study currently involves 14 ICUs in German hospitals. A further five ICUs are awaiting approval for the trial.
The Friedrich Schiller University Jena is the sponsor of the trial. The trial was approved by the ethics committee of the Jena University Hospital on 19 July 2016 and the German Health Authorities (BfArM) on 17 June 2016. In addition, the local ethics committees at each site approved the study protocol and the study competence of each site. Written informed consent is obtained from all patients or their legal representatives. If this is not possible before enrolment in due time, the ethics committees has approved a deferred consent process where the inability to provide consent is confirmed by an independent physician and the patient is enrolled without informed consent. As soon as the legal representative of the patient is available, written informed consent is immediately obtained; otherwise, the patient is withdrawn from the study and all study procedures are ended.
The primary aim of the CandiSep study is to evaluate the impact of BDG-driven antifungal therapy on the rate of death from any cause by 28 days after inclusion.
The secondary objectives are to evaluate the impact of BDG in patients with sepsis on
antifungal-free survival within 28 days after enrolment
Candida colonization according to the Candida colonization index (CCI)
time to antifungal therapy
duration of organ support, including ventilation, vasopressor, and renal replacement, until day 28
mean total score for sequential organ failure assessment (SOFA) calculated as the sum of daily SOFA scores divided by the number of study days on ICU but not longer than 14 days 
ICU and length of hospital stay
ICU and hospital mortality
Frequency of adverse and severe adverse events
Further secondary objectives are to compare
the diagnostic performance of BDG with other laboratory techniques to diagnose ICI early, such as PCR, mannan antigen and anti-mannan antibodies, Candida germ tube antibodies, and other antigen or antibody tests
costs of antifungal therapy between the groups
All patients treated in the ICUs of participating hospitals are eligible for inclusion if they fulfill all the inclusion criteria and none of the exclusion criteria. Patients are screened daily by the study personnel at each study site.
Presence of severe sepsis or septic shock. Sepsis definitions were reported previously . However, in the light of the Sepsis-3 definitions , we do not include the systemic inflammatory response syndrome (SIRS) as a requirement for sepsis diagnosis. Thus, severe sepsis in this trial was defined as microbiologically proven or clinically suspected infection combined with acute organ dysfunction (Table 1). Septic shock was defined as infection in combination with arterial hypotension or need for vasopressor therapy despite adequate fluid resuscitation.
Onset of sepsis: ≤12 h before randomization (until 25 January 2018) or ≤24 h before randomization (since 26 January 2018, after an amendment to the study protocol)
Increased risk of ICI with at least one of the following criteria:
Total parenteral nutrition defined as intravenous infusion of at least 500 ml of 20% glucose per day, maximum of 500 ml enteral feeding per day, no oral feeding other than tea or water
Abdominal surgery within the last 7 days
Antimicrobial therapy ≥48 h within the last 7 days
Continuous need for renal replacement therapy for chronic renal failure or renal replacement therapy for acute renal failure ≥48 h before onset of sepsis
Informed consent of patient or their legal representative or if not possible a statement by an independent physician
Pregnant or lactating women
Proven ICI as defined by the EORTC/MSG-Consensus Group 
Ongoing or immediately planned systemic antifungal therapy
Liver cirrhosis CHILD-Pugh class C
Surgery with cardiopulmonary bypass within the last 4 weeks
Treatment with immunoglobulins within the last 4 weeks
Immunosuppression (e.g., for solid organ transplantation, AIDS, or leukopenia)
Participation in another clinical study
Previous participation in this study
No commitment to full patient support (i.e., do not resuscitate order)
Patient’s death is considered imminent due to coexisting disease
Relationship to study team member (i.e. colleague, relative, or employee)
The study is run by the publicly funded Center for Sepsis Control & Care. The steering committee consists of two intensivists (FB and DTR) and an external expert on fungal infections (OAC). The steering committee is supported by the Center for Clinical Studies of Jena University Hospital, which is responsible for trial management and monitoring the source data. Adherence to the study protocol is ensured by risk-based monitoring. The monitor visits each center three times during the study. The first visit occurs after the first recruitment. In addition, electronic case report forms (eCRFs) are regularly checked by the monitor and the data manager. Adverse and severe adverse events are reported until day 14 or the end of the ICU stay (whatever comes first). Any adverse event not recovered until day 28 is also reported as a severe adverse event. Adverse events are recorded in the eCRF. Severe adverse events are submitted to the trial management via fax within one working day and are assessed by an additional assessor. Severe adverse events related to study procedures or the investigational produce are forwarded to the German authorities.
The data and safety monitoring board (DSMB) is composed of three external experts (an infectious disease physician, a microbiologist, and a statistician). The DSMB is regulated by a standardized operating procedure. The main function of the DSMB is to monitor the safety of the study procedure. Thus, the DSMB receives unblinded quarterly safety reports, which are required under German legislation. The safety report contains descriptive data about serious adverse events. In addition, the DSMB evaluates annually the safety and quality of the ongoing trial by receiving notifications of adverse events, recruitment rates, and protocol violations especially regarding the treatment algorithm of the BDG group. The DSMB advises the sponsor on whether to continue or discontinue the study and on protocol amendments.
Randomization and study procedures
The trial sites have access to a web-based central randomization service, which is available 24 × 7. The randomization list is prepared by an independent statistician via a computer-based algorithm and is stratified by study center. Patients are randomized to one of the two study arms (control group or BDG group) in a 1:1 ratio. A unique patient ID is generated for data collection throughout the trial.
The time flow of the study is shown in Fig. 1 and a time frame of the study procedures are described in Fig. 2. Serum samples for BDG measurements are taken under standardized conditions not later than 1 h after randomization and after 24 h. Samples are left to coagulate for 30 min at room temperature and are then centrifuged at 2000g at 20 °C for 10 min. Serum is filled into BDG-free tubes without using a pipette and refrigerated together with an EDTA blood sample at 2–8 °C. Serum and EDTA samples are shipped at 2–8 °C via courier to the Microbiology Institute of the University Hospital Erlangen. There, BDG serum concentrations are measured using the Fungitell® assay (Associates of Cape Cod Inc., East Falmouth, MA, USA) following the standard operating procedures. Any remaining serum is frozen at −80 °C for later analysis of mannan antigen, anti-mannan antibodies, and Candida germ tube antibodies. EDTA blood samples are frozen at −80 °C for later measurement in additional antigen and antibody assays.
Blood cultures must be obtained by sterile venipuncture at most 3 h after randomization if no blood cultures were taken up to 6 h before randomization. An additional sample for Candida PCR is obtained via the same venipuncture. In addition, microbiological samples are taken from the nose or throat, skin (axillar region), rectum or feces, urine, and tracheal or bronchial secretion to determine the CCI . Blood cultures are repeated on the day after randomization and microbiological samples for the CCI are repeated on day 7 and day 14 if the patient is still in the ICU.
Candida PCR is performed in the National Reference Center for Invasive Mycoses (NRZMyk, Hans-Knöll-Institute, Jena, Germany). EDTA samples are shipped via the regular postal service at room temperature. Candida spp. are detected by a seminested PCR assay amplifying the internal transcribed spacer region 2 (ITS2). Total DNA is extracted from a minimum of 3 ml of EDTA anticoagulated blood . The primary PCR amplifies the entire ITS using the panfungal primers ITS1fkyo2  and ITS4  while for the seminested PCR, the Candida specific primer Cand F  and the panfungal primer ITS4 are used to amplify specifically the ITS2 region of Candida spp. PCR products are detected in agarose gels using GelRed. Species identification is achieved by sequence comparison using in-house ITS alignments including sequences of ex-type and reference strains of all clinically relevant Candida spp. and their sibling species. During the assay, appropriate controls for DNA extraction (negative control) and amplification (negative and positive control) are tested in parallel. PCR results are not reported to the treating physicians.
EDTA and heparin plasma samples are taken daily until day 9 after randomization or the day of ICU discharge, whichever occurs first. Samples are centrifuged at standardized conditions (2000g at 20 °C for 10 min) and are stored locally at −20 °C. Samples are shipped regularly on dry ice via courier to the Integrated Biobank at the Jena University Hospital where samples are stored at −80 °C.
Serum for measurement of BDG concentration is obtained not later than 1 h after randomization and after 24 h. Samples are shipped regularly as convenient. The treating physicians are blinded for the BDG results. Treatment of ICI occurs according to European guidelines based on microbiological findings .
Serum for measurement of BDG concentration is obtained no later than 1 h after randomization and after 24 h. Each sample is shipped to the central laboratory via courier as soon as possible after the sample was obtained. Specific courier schedules depending on the distance to the central laboratory have been developed for each center. These schedules ensure that the result of the first BDG sample is available no later than 24 h after sampling. The treating physicians are informed about the BDG results via telephone and fax. Table 2 describes the therapeutic procedure depending on the BDG results. Briefly, ICI is unlikely if BDG concentrations are less than 80 pg/ml. In this case, antifungal therapy is not required. Any BDG serum concentration of ≥80 pg/ml is compatible with ICI and should be followed by initiation of antifungal therapy, which follows European guidelines . Any blood culture, biopsy, or sample from physiologically sterile body fluids that are positive for Candida spp. are treated independently of the BDG result. The final decision about antifungal therapy is made when all microbiological results from randomization are available using a predefined treatment algorithm (Table 2). If culture results are negative for Candida spp., an initially started empirical antifungal therapy is continued only if serum BDG concentrations are above 80 pg/ml in both samples.
Patients are withdrawn from the study if the patient or the patient’s legal representative withdraws informed consent. In this case, all study interventions are stopped. All data captured until this time point are kept in the database and safety-related data are documented until day 28 if possible. Data from such patients become only part of the safety analysis dataset. Withdrawn patients are replaced by an additionally randomized patient with the same group assignment.
Data are collected via web-based data capture software (OpenClinica®) compliant with good clinical practice requirements. Data are pseudonymized. Only personnel at the study sites have access to the personalized data. Visits and study assessments are shown in Fig. 2. Assessments are made at enrolment (day 0) and for the following 14 days if the patient is still in the ICU. Assessments include parameters to calculate the SOFA score, applied supportive therapy, daily blood chemistry, microbiological samples, antimicrobial therapy as well as adverse and serious adverse events according to German legislation. Adverse and serious adverse events are reported until day 14 after randomization or when the patient is discharged from the ICU, whichever comes first. Patients or relatives are contacted to obtain survival status on day 28 after randomization. We also obtain the duration of antifungal therapy, vasopressor support, mechanical ventilation, and renal replacement therapy.
Sample size and power
This study is designed to reject the null hypothesis that 28-day mortality is equal in the BDG control and in the control group. Studies have shown that 16.8% of patients with a CCI of 3 or more develop ICI . However, it is estimated that only 60% of ICI can be proven in microbiological cultures . We, therefore, estimated that 28% of the high-risk population in this study are developing ICI. In the control group, 97.6% of those patients with ICI are expected to die, as the antifungal therapy is delayed until there is microbiological proof of fungal infection . It is hypothesized that early antifungal therapy triggered by BDG serum concentration will reduce ICI occurrence to 14%  and mortality to 52.8% . The mortality of patients with a high risk for ICI without actually having ICI is estimated to be 31.2% in both groups .
Based on these estimates, the control group has an expected mortality of 49.8% (= 0.28 × 0.976 [mortality of patients having ICI] + 0.72 × 0.312 [mortality of patients not having ICI]) compared to 34.2% (= 0.14 × 0.528 [mortality of patients having ICI] + 0.86 × 0.312 [mortality of patients not having ICI]) in the BDG group. A two-group chi-squared test can detect this difference with alpha = 0.05 with a statistical power of 0.8 in a sample of 156 patients per group. Studies with a similar patient population have demonstrated a 10% drop-out rate [20, 31]. We, therefore, aim to randomize 348 patients to achieve 312 evaluable patients.
The study objectives are analyzed in the intention-to-treat population. The primary objective is analyzed with the chi-squared test. Relative risk, risk difference, and number needed to treat are reported with 95% confidence intervals. Secondary objectives are analyzed according to their scales. A chi-squared or Fisher’s exact test is applied for 28-day antifungal-free survival, Candida colonization, and ICU and hospital mortality. A t-test for independent samples or a Mann–Whitney U-test is applied for the CCI, mean total SOFA score, and organ-support-free days. Kaplan–Meier estimates and log rank tests are applied for time to antifungal therapy, and time to hospital or ICU discharge.
For the following predefined subgroups, 28- day mortality, time to antifungal therapy, and length of hospital stay are separately analyzed:
septic shock at randomization
CCI ≥ 0.5
Candida PCR positive
blood culture positive for Candida spp.
BDG serum concentration ≥ 80 pg/ml in both samples
more than two risk factors for ICI as defined in the inclusion criteria
The diagnostic accuracy of BDG, PCR, and other experimental tests is assessed by calculating sensitivity and specificity together with 95% confidence intervals. ICI is defined as the presence of Candida spp. in a tissue biopsy, in a blood culture, or in a primary sterile bloody fluid. Pharmacoeconomics are assessed by calculating the costs of antifungal therapy for each individual patient and adding the cost of BDG measurement for patients in the BDG group.
Moreover, 28-day mortality, time to antifungal therapy, and length of hospital stay are also analyzed in a per-protocol analysis. For the per-protocol analysis, patients in the BDG group are excluded if antifungal therapy did not comply with the algorithm defined in the protocol (Table 2) and patients in the control group are excluded if they received systemic antifungal therapy in the absence of proven ICI.
European guidelines recommend antifungal therapy only in cases where Candida spp. are detected in physiologically sterile body fluids . If Candida is the underlying pathogen and treated upon microbiological proof only, the mortality of patients with septic shock is extremely high . Because of this high mortality, American guidelines recommend empiric antifungal therapy in patients with a high risk of ICI [8, 9] although evidence for such a recommendation is low and risk factors have not been established. This uncertainty triggers the prescription of antifungals to critically ill patients, which is often not compliant with guidelines . Thus, fast identification of ICI is an urgent need. This demand may be met by measuring BDG serum concentrations. Elevated BDG serum concentrations of more than 80 pg/ml have a high diagnostic accuracy to predict ICI [12, 13, 18, 33, 34]. However, the available data suggest that many ICU interventions may interfere by increasing BDG serum concentrations [11, 14, 15]. In addition, the diagnostic accuracy of BDG has been reported only for candidemia rather than ICI in general and may also depend on the fungal species . These limitations may further affect the clinical usability of BDG. Indeed, the latest Surviving Sepsis Campaign guideline refrained from using BDG serum concentrations to indicate antifungal therapy . Taken together, BDG has the potential to add significant information about Candida spp. as the underlying pathogen in patients with sepsis but conclusive evidence for critically ill patients is missing. As a consequence, the role of BDG remains undefined and BDG measurement is currently not part of the diagnostic work-up of infection in most ICUs.
The trial attempts to enroll patients with a high risk of ICI to increase the chance of including patients who may benefit from the study intervention. Many risk factors for developing ICI have been published , making it difficult to identify patients truly at risk when using all the available risk factors. Firstly, we applied elements of the CCI (total parenteral nutrition and past surgery) as risk factors. An elevated CCI is associated with a high incidence of ICI . Secondly, we performed a meta-analysis of risk factors for ICI and added the most important risk factors (renal replacement therapy and previous antimicrobial therapy) to the elements of the CCI . The meta-analysis also revealed that previous abdominal surgery in particular, rather than any previous surgery, is associated with a high risk of ICI. Multifocal Candida colonization is another known risk factor for developing ICI . However, we did not choose colonization as an inclusion criterion. Surveillance cultures for Candida colonization are not taken in most of the study centers. In addition, microbiological results are usually not available at the onset of sepsis when study enrolment must be decided.
The SEPSIS-3 definition was published during the development of this study . At this stage, we decided not to change the inclusion criteria to the Sepsis-3 definitions, since all sample size calculations relied on studies that were based on the consensus criteria of the American College of Chest Physicians and Society of Critical Care Medicine . We did, however, abandon the requirement for SIRS to qualify for enrolment. The development of acute organ dysfunction as a cause of infection is the main pathophysiological step that determines the prognosis of the patient. SIRS does not affect the outcome and does not predict the development of severe sepsis or septic shock [39,40,41].
The sample size calculation for this study was difficult since ICI often remains unrecognized. ICI is mainly diagnosed as candidemia but deep-seated candidiasis is commonly not accompanied by positive blood cultures . Thus, many assumptions had to be made. We have chosen the approach of the EMPIRICUS trial  to estimate the number of patients who may benefit from early empiric antifungal therapy and replaced the estimates with results from more recent trials. A recent retrospective study on 198 patients with elevated CCI confirmed our estimates. Of these patients, 31.8% had elevated BDG serum concentrations of which 74.6% had proven candidemia. All episodes of candidemia occurred in the elevated BDG group . This aligns quite well with our estimate that 28% of the patients at risk are assumed to develop ICI.
The strengths of this study include the multicenter, randomized, controlled design. The trial is undertaken according to good clinical practice guidelines. The study interventions are based on guideline recommendations, therefore they follow closely clinical practice. The participating hospitals represent standard care in Germany. Thus, the results of this study are generalizable to similar health-care settings.
Our trial has limitations. The current version of the Fungitell® assay does not allow measurement of serum BDG concentrations in each local laboratory. A central laboratory had to be established to ensure the uniform quality of the BDG results. However, such a setup will not always allow us to keep the time to result below 24 h, which is the desired time frame for keeping mortality rates as low as possible . Sample are shipped via a courier service to minimize delays. Certain hours of enrolment are ruled out individually for each center, if timely shipping cannot be guaranteed by the courier service. The time window for study inclusion had to be increased from 12 to 24 h because of a high number of screening failures. This may further increase the time to antifungal therapy in the BDG group and therefore, might reduce the difference in primary and secondary outcomes between the two groups. However, the recruitment rate before the amendment was too low to allow completion of the trial in due time. The study has a risk of performance and detection bias with respect to diagnosing and treating ICI, since the trial design does not allow blinding. The lack of blinding may trigger the treating physician to focus more on ICI due to the BDG reporting in the BDG group. In the control group, however, ICI diagnosis and therapy is left to the discretion of the physician without reminders by the study protocol. Training on the European guidelines regarding the management of ICIs , which all centers agreed to follow, tries to minimize this issue. We measure BDG only at the onset of sepsis. However, ICI might develop later during the course of sepsis. Although it might be helpful for patient management to have BDG guidance available during the whole ICU stay, this was beyond the financial scope of the study.
This is the first randomized controlled study to investigate whether BDG serum concentrations can guide a physician in supplementing the empiric antimicrobial therapy with an antifungal in critically ill patients with severe sepsis or septic shock. This clinical study will generate a solid evidence base for health-care providers and authors of guidelines for BDG in critically ill patients.
The study opened for recruitment on 15 September 2016. As of 31 July 2018, 176 patients (78 patients before the amendment and 98 patients after the amendment) have been enrolled into the study. Completion of recruitment is expected in September 2019.
Candida colonization index
Data and safety monitoring board
Electronic case report form
Invasive Candida infection
Internal transcribed spacer region
Intensive care unit
National Reference Center for Invasive Mycoses
Polymerase chain reaction
Sequential organ failure assessment
Bitar D, Lortholary O, Le Strat Y, Nicolau J, Coignard B, Tattevin P, et al. Population-based analysis of invasive fungal infections, France, 2001-2010. Emerg Infect Dis. 2014;20:1149–55.
Zilberberg MD, Shorr AF, Kollef MH. Secular trends in candidemia-related hospitalization in the United States, 2000-2005. Infect Control Hosp Epidemiol. 2008;29:978–80.
Vincent J-L, Sakr Y, Sprung CL, Ranieri VM, Reinhart K, Gerlach H, et al. Sepsis in European intensive care units: results of the SOAP study. Crit Care Med. 2006;34:344–53.
Zaoutis TE, Argon J, Chu J, Berlin JA, Walsh TJ, Feudtner C. The epidemiology and attributable outcomes of candidemia in adults and children hospitalized in the United States: a propensity analysis. Clin Infect Dis. 2005;41:1232–9.
Clancy CJ, Nguyen MH. Finding the “missing 50%” of invasive candidiasis: how nonculture diagnostics will improve understanding of disease spectrum and transform patient care. Clin Infect Dis. 2013;56:1284–92.
Garey KW, Rege M, Pai MP, Mingo DE, Suda KJ, Turpin RS, et al. Time to initiation of fluconazole therapy impacts mortality in patients with candidemia: a multi-institutional study. Clin Infect Dis. 2006;43:25–31.
Kollef M, Micek S, Hampton N, Doherty JA, Kumar A. Septic shock attributed to Candida infection: importance of empiric therapy and source control. Clin Infect Dis. 2012;54:1739–46.
Pappas PG, Kauffman CA, Andes DR, Clancy CJ, Marr KA, Ostrosky Zeichner L, et al. Clinical practice guideline for the Management of Candidiasis: 2016 update by the Infectious Diseases Society of America. Clin Infect Dis. 2016;62:e1–50.
Rhodes A, Evans LE, Alhazzani W, Levy MM, Antonelli M, Ferrer R, et al. Surviving Sepsis campaign: international guidelines for Management of Sepsis and Septic Shock: 2016. Intensive Care Med. 2017;43:304–77.
Cornely OA, Bassetti M, Calandra T, Garbino J, Kullberg BJ, Lortholary O, et al. ESCMID* guideline for the diagnosis and management of Candida diseases 2012: non-neutropenic adult patients. Clin Microbiol Infect. 2012;18(Suppl 7):19–37.
Wright WF, Overman SB, Ribes JA. (1–3)- -D-Glucan Assay: A Review of its Laboratory and Clinical Application. Lab Med Am Soc Clin Pathol. 2011;42:679–85.
Ostrosky Zeichner L, Alexander BD, Kett DH, Vazquez J, Pappas PG, Saeki F, et al. Multicenter clinical evaluation of the (1->3) beta-D-glucan assay as an aid to diagnosis of fungal infections in humans. Clin Infect Dis. 2005;41:654–9.
Karageorgopoulos DE, Vouloumanou EK, Ntziora F, Michalopoulos A, Rafailidis PI, Falagas ME. β-D-glucan assay for the diagnosis of invasive fungal infections: a meta-analysis. Clin Infect Dis. 2011;52:750–70.
Cascio Lo G, Koncan R, Stringari G, Russo A, Azzini A, Ugolini A, et al. Interference of confounding factors on the use of (1,3)-beta-D-glucan in the diagnosis of invasive candidiasis in the intensive care unit. Eur J Clin Microbiol Infect Dis. 2015;34:357–65.
Liss B, Cornely OA, Hoffmann D, Dimitriou V, Wisplinghoff H. 1,3-ß-D-glucan concentrations in blood products predict false positive post-transfusion results. Mycoses. 2016;59:39–42.
Scudeller L, Bassetti M, Concia E, Corrao S, Cristini F, De Rosa FG, et al. MEDical wards invasive candidiasis ALgorithms (MEDICAL): consensus proposal for management. Eur J Intern Med. 2016;34:45–53.
Knitsch W, Vincent J-L, Utzolino S, François B, Dinya T, Dimopoulos G, et al. A randomized, placebo-controlled trial of preemptive antifungal therapy for the prevention of invasive candidiasis following gastrointestinal surgery for intra-abdominal infections. Clin Infect Dis. 2015;61:1671–8.
Ostrosky Zeichner L, Shoham S, Vazquez J, Reboli A, Betts R, Barron MA, et al. MSG-01: a randomized, double-blind, placebo-controlled trial of caspofungin prophylaxis followed by preemptive therapy for invasive candidiasis in high-risk adults in the critical care setting. Clin Infect Dis. 2014;58:1219–26.
Moreno R, Vincent JL, Matos R, Mendonça A, Cantraine F, Thijs L, et al. The use of maximum SOFA score to quantify organ dysfunction/failure in intensive care. Results of a prospective, multicentre study. Working group on Sepsis related problems of the ESICM. Intensive Care Med. 1999;25:686–96.
Bloos F, Trips E, Nierhaus A, Briegel J, Heyland DK, Jaschinski U, et al. Effect of sodium selenite administration and Procalcitonin-guided therapy on mortality in patients with severe Sepsis or septic shock: a randomized clinical trial. JAMA Intern Med. 2016;176:1266–76.
Singer M, Deutschman CS, Seymour CW, Shankar-Hari M, Annane D, Bauer M, et al. The third international consensus definitions for Sepsis and septic shock (Sepsis-3). JAMA. 2016;315:801–10.
De Pauw B, Walsh TJ, Donnelly JP, Stevens DA, Edwards JE, Calandra T, et al. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of cancer/invasive fungal infections cooperative group and the National Institute of Allergy and Infectious Diseases mycoses study group (EORTC/MSG) consensus group. Clin Infect Dis. 2008;46:1813–21.
Pittet D, Monod M, Suter PM, Frenk E, Auckenthaler R. Candida colonization and subsequent infections in critically ill surgical patients. Ann Surg. 1994;220:751–8.
Springer J, Loeffler J, Heinz W, Schlossnagel H, Lehmann M, Morton O, et al. Pathogen-specific DNA enrichment does not increase sensitivity of PCR for diagnosis of invasive aspergillosis in neutropenic patients. J Clin Microbiol. 2011;49:1267–73.
Toju H, Tanabe AS, Yamamoto S, Sato H. High-coverage ITS primers for the DNA-based identification of ascomycetes and basidiomycetes in environmental samples. PLoS ONE. 2012;7:e40863.
White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press; 1990. p. 315-22.
Schabereiter-Gurtner C, Selitsch B, Rotter ML, Hirschl AM, Willinger B. Development of novel real-time PCR assays for detection and differentiation of eleven medically important aspergillus and Candida species in clinical specimens. J Clin Microbiol. 2007;45:906–14.
León C, Ruiz-Santana S, Saavedra P, Galván B, Blanco A, Castro C, et al. Usefulness of the “Candida score” for discriminating between Candida colonization and invasive candidiasis in non-neutropenic critically ill patients: a prospective multicenter study. Crit Care Med. 2009;37:1624–33.
Schuster MG, Edwards JE, Sobel JD, Darouiche RO, Karchmer AW, Hadley S, et al. Empirical fluconazole versus placebo for intensive care unit patients: a randomized trial. Ann Intern Med. 2008;149:83–90.
Bloos F, Bayer O, Sachse S, Straube E, Reinhart K, Kortgen A. Attributable costs of patients with candidemia and potential implications of polymerase chain reaction-based pathogen detection on antifungal therapy in patients with sepsis. J Crit Care. 2013;28:2–8.
Brunkhorst FM, Oppert M, Marx G, Bloos F, Ludewig K, Putensen C, et al. Effect of empirical treatment with moxifloxacin and meropenem vs meropenem on sepsis-related organ dysfunction in patients with severe sepsis: a randomized trial. JAMA. 2012;307:2390–9.
Azoulay E, Dupont H, Tabah A, Lortholary O, Stahl J-P, Francais A, et al. Systemic antifungal therapy in critically ill patients without invasive fungal infection*. Crit Care Med. 2012;40:813–22.
Nguyen MH, Wissel MC, Shields RK, Salomoni MA, Hao B, Press EG, et al. Performance of Candida real-time polymerase chain reaction, β-D-glucan assay, and blood cultures in the diagnosis of invasive candidiasis. Clin Infect Dis. 2012;54:1240–8.
Posteraro B, De Pascale G, Tumbarello M, Torelli R, Pennisi MA, Bello G, et al. Early diagnosis of candidemia in intensive care unit patients with sepsis: a prospective comparison of (1→3)-β-D-glucan assay, Candida score, and colonization index. Crit Care. 2011;15:R249.
Delaloye J, Calandra T. Invasive candidiasis as a cause of sepsis in the critically ill patient. Virulence. 2014;5:1–9.
Bloos F, Schlattmann P, Pletz M, Kurzai O, Thomas-Rüddel D. Meta-analysis of risk factors for developing invasive Candida infection in adult critically ill patients. Mycoses. 2015;58(Suppl. 3):8.
Muskett H, Shahin J, Eyres G, Harvey S, Rowan K, Harrison D. Risk factors for invasive fungal disease in critically ill adult patients: a systematic review. Crit Care. 2011;15:R287.
Bone RC, Sibbald WJ, Sprung CL. The ACCP-SCCM consensus conference on sepsis and organ failure. Chest. 1992;101:1481–3.
Pittet D, Rangel-Frausto S, Li N, Tarara D, Costigan M, Rempe L, et al. Systemic inflammatory response syndrome, sepsis, severe sepsis and septic shock: incidence, morbidities and outcomes in surgical ICU patients. Intensive Care Med. 1995;21:302–9.
Brooks D, Smith A, Young D, Fulton R, Booth MG. Mortality in intensive care: the impact of bacteremia and the utility of systemic inflammatory response syndrome. Am J Infect Control. 2016;
Seymour CW, Liu VX, Iwashyna TJ, Brunkhorst FM, Rea TD, Scherag A, et al. Assessment of clinical criteria for Sepsis: for the third international consensus definitions for Sepsis and septic shock (Sepsis-3). JAMA. 2016;315:762–74.
Timsit JF, Azoulay E, Cornet M, Gangneux J-P, Jullien V, Vesin A, et al. EMPIRICUS micafungin versus placebo during nosocomial sepsis in Candida multi-colonized ICU patients with multiple organ failures: study protocol for a randomized controlled trial. Trials. 2013;14:399.
Posteraro B, Tumbarello M, De Pascale G, Liberto E, Vallecoccia MS, De Carolis E, et al. (1,3)-β-d-Glucan-based antifungal treatment in critically ill adults at high risk of candidaemia: an observational study. J Antimicrob Chemother. 2016;71:2262–9.
The authors thank the staff at the Center for Clinical Studies of Jena University Hospital for their contribution to the trial infrastructure and especially Cornelia Eichhorn for the eCRF development. Michael Kiehntopf runs the Integrated Biobank at Jena University Hospital and was of invaluable help in organizing the pre-analytic procedures for the plasma biobank used in the trial. The authors thank Associates of Cape Cod Inc. (East Falmouth, MA, USA) for supplying the BDG kits. The authors thank the members of the DSMB board for their support: Prof. Dr. Elisabeth Presterl, MBA (Medical University Vienna, Austria, Dept. for Hospital Hygiene and Infection Control), Dr. Florian Prüller (University Hospital Graz, Switzerland, Dept. of Medical and Chemical Laboratory Diagnostics), Prof. Dr. Walter Lehmacher (University Cologne, Germany, Dept. of Medical Statistics, Informatics and Epidemiology).
The study is funded by the Federal Ministry of Education and Research (grant 01EO1502). The funding bodies had no role in the design of the study, in the collection, analysis, and interpretation of the data, or in writing the manuscript. The NRZMyk is supported by the Robert Koch Institute from funds provided by the German Ministry of Health (grant 1369–240).
Availability of data and materials
The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request. The transfer of data requires a positive vote by the local ethics committee and the data protection board.
Consent for publication
We have read and understood the TRIALS policy on declarations of interests and declare the following interests. FB, DTR, and OK belong to the institution receiving public funding for the trial. FB has received lecture honoraria from Biosyn, Gilead, and CSL Behring. JH received royalties for lectures from MSD as well as lecture royalties and a research grant from Pfizer. OAC is an unpaid member of the European Confederation of Medical Mycology and of the European Fungal Infection Study Group of the European Society for Clinical Microbiology and Infectious Diseases, which are developing joint guidelines. OAC has received research grants from Actelion, Amplyx, Arsanis, Astellas, AstraZeneca, Basilea, Bayer, Cidara, Duke University (NIH UM1AI104681), F2G, Gilead, GSK, Leeds University, Matinas, Medicines Company, MedPace, Melinta, Merck/MSD, Miltenyi, Pfizer, Rempex, Roche, Sanofi Pasteur, Scynexis, and Seres. OAC is a consultant to Amplyx, Actelion, Astellas, Basilea, Cidara, Da Volterra, F2G, Gilead, Janssen, Matinas, Menarini, Merck/MSD, Paratek, PSI, Scynexis, Seres, Summit, Tetraphase, and Vical. OAC has received lecture honoraria from Astellas, Basilea, Gilead, Merck/MSD, and Pfizer. NB and PS report no conflicts of interest.
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Bloos, F., Held, J., Schlattmann, P. et al. (1,3)-β-D-glucan-based diagnosis of invasive Candida infection versus culture-based diagnosis in patients with sepsis and with an increased risk of invasive Candida infection (CandiSep): study protocol for a randomized controlled trial. Trials 19, 472 (2018). https://doi.org/10.1186/s13063-018-2868-0
- Septic shock
- Invasive Candida infection
- Early antifungal therapy