Effect of Pycnogenol® on attention-deficit hyperactivity disorder (ADHD): study protocol for a randomised controlled trial
© The Author(s). 2017
Received: 21 November 2016
Accepted: 6 March 2017
Published: 28 March 2017
Methylphenidate (MPH), the first choice medication for attention-deficit hyperactivity disorder (ADHD), is associated with serious adverse effects like arrhythmia. Evidence on the association of ADHD with immune and oxidant-antioxidant imbalances offers potential for antioxidant and/or immunomodulatory nutritional supplements as ADHD therapy. One small randomised trial in ADHD suggests, despite various limitations, therapeutic benefit from Pycnogenol®, a herbal, polyphenol-rich extract.
This phase III trial is a 10-week, randomised, double-blind, placebo and active treatment controlled multicentre trial with three parallel treatment arms to compare the effect of Pycnogenol® to MPH and placebo on the behaviour of 144 paediatric ADHD and attention-deficit disorder (ADD) patients. Evaluations of behaviour (measured by the ADHD-Rating Scale (primary endpoint) and the Social-emotional Questionnaire (SEQ)), immunity (plasma cytokine and antibody levels, white blood cell counts and faecal microbial composition), oxidative stress (erythrocyte glutathione, plasma lipid-soluble vitamins and malondialdehyde and urinary 8-OHdG levels, as well as antioxidant enzyme activity and gene expression), serum zinc and neuropeptide Y level, urinary catecholamines and physical complaints (Physical Complaints Questionnaire) will be performed in week 10 and compared to baseline. Acceptability evaluations will be based on adherence, dropouts and reports of adverse events. Dietary habits will be taken into account.
This trial takes into account comorbid behavioural and physical symptoms, as well as a broad range of innovative immune and oxidative biomarkers, expected to provide fundamental knowledge on ADHD aetiology and therapy. Research on microbiota in ADHD is novel. Moreover, the active control arm is rather unseen in research on nutritional supplements, but of great importance, as patients and parents are often concerned with the side effects of MPH.
KeywordsADHD ADD Behaviour Pycnogenol® Antioxidant Polyphenols Oxidative stress Immunity Catecholamines
Attention-deficit hyperactivity disorder (ADHD) is a common neurocognitive behavioural disorder with childhood onset and core symptoms of hyperactivity, impulsivity and inattention . ADHD has a worldwide prevalence of 5.9–7.1% and is associated with other psychiatric disorders, such as oppositional defiant disorder (ODD), autism and anxiety [2, 3].
Methylphenidate (MPH), the first-choice medication for ADHD, is a central nervous system stimulant. It increases attentiveness and reduces hyperactivity and impulsivity by inhibition of dopamine reuptake in the striatum, without triggering its release. MPH is prescribed for chronic use to a large proportion of ADHD patients, but is linked to possible publication bias in reported efficacy [4–6]. In addition, parents are often disinclined to use MPH due to its negative publicity and its frequent side effects, including serious side effects like arrhythmia, and, subsequently, nonadherence to therapy is high [4–7]. A recent review reports adverse effects, like insomnia and decreased appetite, in about 25% of patients using MPH . Other therapeutic options are therefore warranted, at least for a subgroup of patients [4, 5, 7, 8].
ADHD is a complex and multifactorial disorder, influenced by both genetics and the environment. Its exact pathophysiology remains, however, unclear. Dopaminergic dysfunction is involved, but also associations with immune and oxidant-antioxidant imbalances exist [9, 10]. Various studies demonstrated, for example, increased levels of plasma malondialdehyde (MDA) and exhalant ethane (oxidative stress markers) and decreased activity of antioxidant enzymes such as glutathione peroxidase (GPX) and catalase (CAT) [11–14]. ADHD has also been hypothesised to be a hypersensitivity disorder, with a disrupted immune regulation contributing to its aetiology ; i.e. ADHD has comorbidity with both Th1- and Th2-mediated disorders and several related genes have immune functions [9, 10, 15–18]. Ceylan et al. observed increased levels of adenosine deaminase, a marker of cellular immunity, and of the oxidative enzymes xanthine oxidase (XO) and nitric oxide synthase, and decreased levels of the antioxidant enzymes glutathione-S-transferase and paraoxonase-1. These results indicate the involvement of oxidative changes and cellular immunity in ADHD .
Still, specific immune biomarkers other than antibodies have not been systematically studied in ADHD despite growing evidence on associations in autism [19, 20]. In addition, immune and oxidative effects of both standard therapy and nutritional supplementation in ADHD are neglected topics in research. Yet, immune and oxidative imbalances linked with ADHD offer potential for appropriate supplementation in ADHD therapy .
Due to its antioxidant and immunomodulatory properties, a commercially available standardised extract from French maritime pine (Pinus pinaster) bark with a high content of polyphenolic compounds (including phenolic acids and procyanidins), Pycnogenol®, was selected for this study [22–24]. One small randomised trial and few observational studies suggest its therapeutic benefit in ADHD. Still, this trial had some limitations (e.g. short supplementation period) and the mechanisms of action involved remain unclear [22, 25–29]. The efficacy, mechanism(s) of action and value of Pycnogenol® in ADHD as compared to MPH treatment thus remain to be confirmed.
To evaluate the effect of Pycnogenol® on ADHD and ADD behaviour and comorbid physical and psychiatric symptoms, as well as on immunity, oxidative damage, antioxidant status and neurochemical parameters, as compared to placebo and MPH treatment.
In ADHD therapy, Pycnogenol® is more effective than placebo and not less effective than MPH
As compared to placebo and MPH, Pycnogenol® increases antioxidant levels, reduces oxidative damage, improves immune and neurochemical status and reduces comorbid physical and psychiatric complaints
The tolerability of Pycnogenol® is higher than that of MPH
This is a phase III, randomised, double-blind, placebo and active product controlled, multicentre clinical trial with three parallel treatment arms to compare effects on ADHD and ADD behaviour between Pycnogenol®, MPH (Medikinet® Retard) and placebo, using the ADHD-Rating Scale (ADHD-RS) as a primary outcome measure. Secondary outcome measures are comorbid physical and psychiatric complaints (including side effects), oxidative stress, immunity, neurochemical parameters and tolerance of the intervention. Following screening and baseline assessments, 144 patients (aged 6–12 years) will receive one of the three treatments for 10 weeks (see Table 2). Evaluations will be performed in weeks 5 and 10, as compared to baseline. Dietary habits will be taken into account.
Two visits with similar evaluations and sample collections will be conducted: at baseline and after 10 weeks. To analyse biomarkers of interest, 16 ml of venous blood will be collected at the start and the end of intervention, as well as urine. Faecal samples will be collected from participant subgroups (n = 60). Next to baseline and final evaluations, an extra evaluation of behaviour and physical symptoms will be conducted in week 5 by means of questionnaires. Two reminders will be sent in case questionnaires are not received within 1 week after the required date. After every blood and urine collection and when questionnaires are completed, participants will receive two movie tickets.
Inclusion and randomisation
Inclusion and exclusion criteria for patient selection
1. Age 6–12 years (both inclusive)
1. Diagnosis of autism spectrum disorder
2. ADHD diagnosis a. based upon the diagnostic interview by the investigating physician b. based upon the ADHD-RS
2. Pervasive developmental disorder, personality disorder, IQ <70, conduct disorder (CD), tics, schizophrenia, dyskinesia, personal or family history of psychotic disorder, bipolar illness, depression, or suicide attempt
3. Responsible caregiver to provide information about the patient’s functional status
3. Chronic medical disorder or acute inflammatory disease. Glaucoma, heart disease, high blood pressure, or peripheral vascular disease
4. Patient and responsible caregiver have a sufficient level of knowledge of Dutch
4. Use of MAO inhibitor 14 days before inclusion. Use of clonidine, guanethidine, seizure medicine, antidepressants, blood thinners, blood pressure, or diet medication 3 months before inclusion
5. Written informed consent by the patient’s legally accepted representative
5. Use of vitamin/mineral/herbal/omega-3 supplements or any medication for longer than 1 week, 3 months before inclusion
6. Other contraindications for MPH or Pycnogenol®, as defined in the Summary of Product Characteristics and Investigator’s Brochure, respectively
MPH (Medikinet® Retard, methylphenidate hydrochloride modified release): patients with a body weight below 30 kg will receive 20 mg/day, those with a body weight of 30 kg or over, 30 mg/day. Treatment during the first week always contains 10 mg, increasing by 10 mg per week to limit side effects
Pycnogenol®: patients with a body weight below 30 kg will receive 20 mg/day, those with a body weight of 30 kg or over, 40 mg/day, aiming at a daily dose of 1 mg/kg and taking into account formulation issues . Treatment during the first 2 weeks always contains 20 mg
Placebo: contains excipients only
In case of adverse events, the investigator, principal caregiver and participant can decide to discontinue the trial medication/supplement. However, no dose adjustment will be performed. Using a standardised questionnaire, adverse events will be documented at weeks 5 and 10, also taking into account the patient’s medical records. Additionally, spontaneously reported adverse events will be recorded. In case of a serious adverse event, the trial code will be broken and treatment discontinued. In case 10% of participants experience a potentially related serious adverse event, the trial will be discontinued.
Pycnogenol® and placebo will be produced in capsules identical to Medikinet® Retard (Medice GmbH). All treatments will be provided in identical jars, labelled with the subject’s trial number and week of intake. Compliance will be determined based on accountability of investigational products and self-reported adherence.
Investigations and data acquisition during the trial
Inclusion and exclusion criteria
Current use of medication/supplements
Blood and urine collection
Lipid-soluble antioxidants analysis
Antioxidant enzyme activity
PBMC count and reactivity analysis
Microbial composition analysis
Summed ADHD score of the ADHD-RS, rated by parents
Summed ADHD score of the Social-emotional Questionnaire (SEQ), rated by parents and teachers
Scores on ADHD subscales of the ADHD-RS and SEQ, rated by parents and teachers (hyperactivity, impulsivity and inattention)
Percentage of responders rated by parents and teachers, defined as participants with a reduction of at least 20% of their baseline summed ADHD-RS score 
Other objectives are to evaluate the effect of Pycnogenol® compared to placebo and MPH on comorbid psychiatric and physical complaints, antioxidant levels, oxidative damage, immunity and neurotransmitters. Other secondary outcomes are, therefore:
Physical and sleep complaints, including various potential side effects, measured by the Physical Complaints Questionnaire (PCQ) 
Erythrocyte glutathione (GSH) level, the most important intracellular antioxidant, analysed by HPLC with electrochemical detection 
Gene expression, quantified by RT-qPCR, focusing on networks counteracting oxidative stress (GPX, CAT, superoxide dismutase (SOD), XO) and stress-related proteins (clusterin, apolipoprotein J) [41, 42]
Serum zinc level, analysed by atomic absorption spectroscopy (AAS) 
Plasma cytokines for monocytes (interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12, tumour necrosis factor (TNF)-α) and T-cells (IL-4, IL-5, IL-6, IL-10, interferon (IFN)-γ) as well as antibody levels (IgA1–2, IgG1–4, IgE) by flow cytometry and ELISA, as markers of immune activation state and skewing [21, 46, 47]
Identification of peripheral blood mononuclear cells (PBMCs) like neutrophils, CD4, CD8 and B-cells and measurement of their functional responses (e.g. cytokine release) after stimulation, as a marker of immune activation state, skewing and responsivity [21, 46, 47]
Intestinal microbial composition, assessed using extreme throughput multiplexed sequencing of 16S ribosomal ribonucleic acid (RNA) gene pools polymerase chain reaction (PCR)-amplified from intestinal content samples [48, 49]
The final objective is to investigate the acceptability of Pycnogenol® compared to Medikinet® Retard and placebo, based on the prevalence of side effects, treatment adherence (defined as more than 90% ingestion as scheduled) and proportion of dropouts.
Dietary habits of participants, such as consumption of vegetables, chocolate, fruit, etc., will be assessed by a Food Frequency Questionnaire (FFQ) at the start and end of intervention [52, 53], to assess potential dietary adaptations during the study as well as baseline differences between treatment groups. The highest educational achievement of both parents will be determined as a proxy for socioeconomic status.
Patients improve by 0.75 standard deviation (SD) on the ADHD-RS summed ADHD score as rated by teachers if using Pycnogenol® for 10 weeks [22, 54], which corresponds to a 20% improvement with active treatment as compared to placebo
Power of 80%, dropout of 20%
Two-sided testing, at a significance level of 0.05 with Bonferroni post-hoc testing correction
Based on these considerations, 48 patients per group will be necessary (n = 144 in total).
Data will be checked for outliers. Missing data will not be accounted for. The three groups will be compared with regard to baseline characteristics. A two-way analysis of variance (ANOVA) will be performed to investigate a potential interaction between treatment and weight. Change in ADHD-RS score as rated by teachers (primary outcome measure) will be compared between the three groups by means of a one-way ANOVA (categories: group, time; α = 0.05) with post-hoc testing. Changes regarding secondary target variables will also be compared between the three groups, by one-way ANOVA with post-hoc analysis with multiple testing correction, Kruskal-Wallis, or Fisher’s exact test. Separate analyses for subgroups (e.g. based on gender, severity of ADHD, dietary habits, etc.) will be performed. Noninferiority of Pycnogenol® as compared to Medikinet® Retard will be demonstrated when the difference in effect on ADHD-RS score is no more than 5 points . This wide margin might be justified due to frequent side effects of MPH. Noninferiority will only be accepted if supported by both intention-to-treat and per-protocol analyses [56, 57].
Ethics and registration
Ethical approval has been obtained in UZA (EC 15/35/365), ZNA (EC approval 4656) and UZ Ghent (2016/0969). The trial has been registered at ClinicalTrials.gov (NCT02700685) and EudraCT (2016-000215-32).
Trial management and research team
The University of Antwerp (Laboratory of Nutrition and Functional Food Science) is the sponsor of this trial, with NH being the coordinating investigator. As principle investigators, BC, DVW and HV will be primarily responsible for patient inclusion. NH and AV are responsible for the analysis of oxidative stress and neurological biomarkers and questionnaire results, as well as data management. HS is responsible for the analysis of immune biomarkers and genetics. No Data Monitoring Committee will be set up. BC, HV, DVW, NH and AV will discuss potential issues regarding, e.g. subject recruitment.
For more information, see both the Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) Checklist and figure (Additional file 1 and Table 2), with more detailed information on the execution of the trial, and scientific, ethical and administrative elements.
This randomised controlled trial addresses the therapeutic potential of a herbal extract in ADHD by investigating its efficacy, mechanism of action and value as compared to standard treatment and placebo. Results can be partly compared to a previously conducted study [22, 28, 29]. A double-blind design was chosen to avoid bias, due to the subjectivity of questionnaire responses. Behavioural assessment by teachers is preferred as primary objective due to the higher sensitivity of teachers’ ratings [58, 59]. A 10-week treatment is considered long enough to see clear effects of both Pycnogenol® and Medikinet® Retard, though still minimising the patient burden and thus maximising compliance . The parallel design was, therefore, also chosen to reduce patient burden.
Pycnogenol® is a patented, proprietary powder extract made exclusively from French maritime pine bark by Horphag Research (Geneva, Switzerland). The extract is standardised to contain 70 ± 5% procyanidins. Pharmacological studies employing in vitro, animal and/or human models have found potent antioxidant activity, anti-inflammatory actions, improvement of endothelial function, etc. . Pycnogenol® was selected for the present study based on previous research suggesting its therapeutic benefits in ADHD, though this trial had several limitations [22, 27–29]. Further research is needed to investigate its efficacy, mechanism of action and value, especially compared to MPH treatment. For example, dietary polyphenols and their metabolites exert prebiotic-like effects, stimulating the growth of intestinal microbiota, which play a fundamental role in immunity [48, 61, 62]. Also the Pycnogenol® dosage is based upon this previous clinical trial, using 1 mg/kg body weight . In the present trial, due to practical reasons, 0.67–1.33 mg/kg body weight will be applied.
Despite being the first choice medication for ADHD, MPH is associated with various adverse effects (including serious adverse events), some of them frequently occurring, including irritability, insomnia, loss of appetite and headache . Based on data from 70 human clinical studies on 5723 healthy subjects and patients, the overall frequency of adverse side effects due to Pycnogenol® is very low (1.8%) and unrelated to dose or duration of use. The majority of adverse effects observed are mild. Gastrointestinal discomfort, the most frequently occurring adverse effect, may be avoided by taking Pycnogenol® with or after meals. In children with ADHD, 2 of 41 Pycnogenol®-supplemented participants experienced side effects (rise of slowness and moderate gastric discomfort). Pycnogenol® did not cause any significant changes in blood pressure or heart rate in four clinical studies (total n = 185). There have been no reports of serious adverse effects since its introduction into the European market around 1970 . Safety trials have demonstrated the absence of mutagenic and teratogenic effects, no perinatal toxicity and no negative effects on fertility . Therefore, the use of Pycnogenol® in children is considered to be safe.
The ADHD-RS is validated and internationally accepted, and consists of nine inattention and nine impulsivity and hyperactivity items based on the Diagnostic and Statistical Manual of Mental Disorders (DSM), each marked out on a four-point rating scale . The ADHD-RS allows comparison of results to those of previously performed trials [22, 54].
In addition to the ADHD-RS, the SEQ is used in this trial. Though this increases the number of questions on behaviour significantly (72 questions), the SEQ is a behaviour evaluation list to assess core symptoms of social-emotional problems, including frequently occurring psychiatric comorbidities of ADHD. Besides ADHD, three other clusters of social-emotional problems are incorporated in the SEQ (social behaviour problems, anxiety and autism), with items covering the core symptoms of these clusters according to DSM. The SEQ can be used for screening, diagnosis and treatment evaluation. Items are rated on a five-point scale .
The approved PCQ consists of 36 questions, of which 18 items are relevant with respect to specific physical and sleep complaints, including eight domains: (1) pain (e.g. headache), (2) unusual thirst or perspiration, (3) eczema, (4) asthma or rhinitis, (5) skin problems (e.g. blotches in the face), (6) tiredness, (7) gastrointestinal problems and (8) sleep problems. Items, including various potential adverse effects, are rated on a five-point scale at baseline and after 5 and 10 weeks . In addition, parents will be asked whether the participant experienced any illness during the trial, what illness, whether any medication was taken, and the type, dose and duration of medication intake.
The FFQ consists of 50 questions on different food groups to be rated on a nine-point scale by parents at the start and end of the intervention, to assess baseline dietary habits and potential adaptations during the study, as well as to relate potential differential effects of Pycnogenol® to dietary polyphenol intake [52, 53]. Insight in global dietary habits (e.g. whether or not the participant consumes fresh fruit on a daily basis) is, therefore, aimed for.
Patients and especially their parents are often worried about side effects of MPH, the standard medication for ADHD. It is, therefore, important to take into account side effects of Pycnogenol® and effects on comorbid complaints. In addition, the behavioural effects of Pycnogenol® compared to placebo, but also compared to MPH, will be investigated. This active control is rather unseen in research on nutritional supplements, but of great importance. In one previous trial, the effect of Pycnogenol® was compared to MPH and placebo. However, neither MPH nor Pycnogenol® outperformed placebo, possibly due to the short treatment period of 3 weeks .
Most research on nutritional supplements or medication in ADHD predominantly assesses effects on ADHD behaviour. This trial, however, takes into account comorbid behavioural and physical symptoms, such as ODD, anxiety and side effects, as well as a broad range of innovative immune, oxidative and neurochemical biomarkers. The analysis of gene expression and biomarkers can indicate genetic effects and biological processes involved in the mechanism of action of Pycnogenol® and possibly affecting ADHD symptom expression. Research on microbiota in ADHD in itself is novel, too. Results of this project will, therefore, increase insight in ADHD aetiology and (dietary) treatment options, which is highly desired by medical staff, parents and patients.
Not yet recruiting as of February 2016.
Atomic absorption spectroscopy
Attention-deficit hyperactivity disorder
Analysis of variance
Diagnostic and Statistical Manual of Mental Disorders
Enzyme-linked ImmunoSorbent Assay
Food Frequency Questionnaire
Physical Complaints Questionnaire
Real-time quantitative polymerase chain reaction
Tumour necrosis factor
- UZ Ghent:
University Hospital Ghent
University Hospital Antwerp
Hospital Network Antwerp
The authors would like to thank Prof. Dr. P. Rohdewald and physicians and co-workers of UZA, UZ Ghent and ZNA for their useful comments and help. The authors would also like to thank Horphag Research Ltd. (Geneva, Switzerland), producer of Pycnogenol®, for the use of Pycnogenol® and scientific advice regarding its use, as well as for the production of the treatments (acquisition, blinding and randomisation of the three treatments, though under supervision of the University of Antwerp).
This paper is dedicated to the memory of our colleague Sandra Apers (°19 August 1972 to ✝5 February 2017).
AV received a PhD scholarship from the Fund for Scientific Research Flanders (Fonds Wetenschappelijk Onderzoek (FWO); FWO MAND 2013 - 11U8316N 5 W).
Availability of data and material
No data obtained yet.
NH, HS and AV initiated the study, in discussion with BC, DVW and HV. AV drafted the manuscript. NH, LP, HS, BC, DVW, TDB and HV critically reviewed the manuscript for final submission. All authors have read the final version and approve its submission.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
Ethical approval has been obtained in the University Hospitals of Antwerp (UZA; EC 15/35/365) and Ghent (UZ Ghent; 2016/0969), as well as in Hospital Network Antwerp (ZNA; EC approval 4656). Written informed consent of the participant’s legal representative to participate in the trial will be obtained before inclusion.
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