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Prospective clinical study of rehabilitation interventions with multisensory interactive training in patients with cerebral infarction: study protocol for a randomised controlled trial



Multisensory interactive training has an increasingly prominent role in stroke rehabilitation. Currently, there is insufficient evidence to demonstrate its efficacy on gait improvement, upper limb and lower limb functional improvement, global motor function and cognitive improvement. A recent Cochrane review confirmed that published studies on virtual reality (VR) training have the limitations of lack of powered sample size, did not evaluate the benefits over a long-term period and lacked trial quality on cognitive function. Another systematic review also concluded that the evidence for the use of VR in gait and balance improvement is limited. This study investigates the effects of multisensory training on gait pattern, upper and lower limb biomechanics, upper limb gross and fine motor functions, and lower limb functional recovery over a medium- to long-term period.


Two hundred and twenty-four acute stroke patients will be recruited from a single centre over a period of 6 years. Participants will be randomly assigned to either conventional therapy or conventional therapy with VR training. Outcomes will be recorded at baseline, post intervention and at 3, 6 and 12 months post intervention. Primary outcome measure is gait speed. Secondary outcome measures include kinematic data of upper and lower limb motion, muscle tone, Action Research Arm Test and Short Orientation Memory Concentration Test.


The results of this trial will provide in-depth understanding of the effect of early VR interventions on gait, upper and lower limb biomechanics and how it may relate to changes in functional outcomes and muscle tone.

Trial registration

Chinese Clinical Trial Registry (Registration No.: ChiCTR-IOC-15006064). Registered on 11 May 2015.

Peer Review reports


Stroke is the third leading cause of disability worldwide [1, 2]. Stroke survivors often have an abnormal hemiparetic gait pattern that is characterised by decreased gait speed [3], altered kinematics of hip, knee and ankle joints during a gait cycle [4] and functional impairment of lower and upper extremities. Impaired ability to walk is one of the most devastating consequences and gait recovery is often regarded as a primary objective in stroke rehabilitation [5]. It is becoming widely recognised that multisensory VR training is an important component of stroke rehabilitation and one that has demonstrated potential to promote gait, lower and upper limb functional recovery, and thus increase quality of life.

Virtual reality (VR) has been subjected to intense research over the past decade as an intervention to promote functional recovery for patients with stroke. The virtual environment offers multisensory interaction and goal-orientated tasks that can stimulate active repetitive movements and offer instant feedback [6]. VR is relatively low cost and may be employed within home settings where patients live remotely or have other barriers to participate in intervention [7]. The concept of using commercial gaming (CG) systems to deliver VR interventions at home as part of the rehabilitation programme was well received among stroke survivors as they believed it to be beneficial for functional recovery [8]. Previous studies on VR interventions indicated that they may be more effective in improving gait, lower limb functions [9, 10] and upper limb functions [11] when compared to conventional therapy. Functional imaging studies have indicated that cortical reorganisation is associated with gait improvement post-VR interventions. The latest published meta-analysis [7] suggested that VR interventions are at least as effective as conventional physiotherapy in improving lower limb outcomes within the stroke population.

Gait performance is a predictor for disability [12, 13], mortality and morbidity [14]. Early intervention with physical therapy to restore gait function had been shown to improve motor function and decrease the subsequent disability when compared to intervention provided at the subacute or chronic stages [15]. Despite the reported positive results for VR interventions, published systematic reviews concluded that the evidence for VR in gait, lower limb functions and balance improvement is limited [16], particularly for stroke patients during the acute stage [17]. It was argued that since most of the neuroplasticity activity took place within the first month of stroke occurrence, VR intervention provided during the acute stage of stroke is likely to lead to better clinical outcomes for gait and lower limb functions [18]. A Cochrane review [19] also concluded that there was insufficient evidence to demonstrate the efficacy of VR training on gait speed and global motor function improvement due to a lack of powered sample size and did not evaluate the effectiveness of VR over a long-term period or post hospital discharge. Thus, little is currently known about the impact of VR on gait and lower limb functions during the acute stage and if the impact is sustainable in the long term.

The recovery of upper limb function to perform delicate motions, such as grasping, finger pinching and individual finger movement continues to be a challenge for stroke survivors [11]. Existing rehabilitation programmes, such as constrain-induced therapy, imagery training and bilateral training, have focussed on the improvement of upper limb gross motor skills [2022]. These training regimes have insufficient emphasis on fine motor skills and coordination of limb movements; thus, leading to reduced ability to carry out activities of daily living. Sophisticated robotic devices have been developed to provide the possibility to train hand and finger motor skills [11]. However, these types of devices are not easily accessible in most rehabilitation departments. In addition, fine motor movement involves the combination of cognitive and motor functions to plan and execute it. Cognitive function refers to the ability to process received information, and to take appropriate action accordingly [12]. Large numbers of stroke survivors have cognitive impairment which presents an additional challenge in upper limb functional recovery. Previous studies have indicated that cognitive function can be recovered through repetitious training [13]. Two Cochrane reviews [19, 23] suggest that VR interventions appear to be promising with moderate-quality evidence to support the beneficial effects on upper limb functional improvement. However, the number of studies available are too few and too small to draw conclusions on upper limb recovery and cognitive function improvements induced by VR interventions. In addition, most of the studies assessed gross motor function by functional outcome measures such as the Action Research Arm Test and the Fugl-Meyer Assessment of Motor Recovery. While they are validated clinical tests, several authors cast doubts on the use of functional outcomes to study motor recovery post stroke. It was argued that functional outcome measures only focussed on task accomplishment regardless of the quality of movement [24, 25]. Since motor compensation can affect [26, 27] task accomplishment, it is, therefore, essential to understand the impact of VR on motor performance measures and to cross reference with functional outcome measures.


The primary aim of this study is to test the hypotheses that patients with stroke who receive VR training during the acute stage will have significantly higher improvement in gait speed, lower limb motor functions and upper limb gross and fine motor functions when compared to those who receive conventional therapy. The observed improvements in the VR group will remain significantly higher than the conventional therapy group at 3-, 6- and 12-month follow-up periods. The secondary aim of this study is to assess the changes of the kinematic characteristics of gait and upper limb motions of ‘reach forward’ and ‘reach forward and grasp’ after 3 weeks of VR interventions and at 3-, 6- and 12-month follow-up periods.


Study design

This study is a single-blind randomised controlled trial (RCT) to compare the differences between two parallel groups.

Study setting

This is a single-centre study which will be conducted by the Rehabilitation Department of the Sun Yat-sen University, China. Participants will be recruited from the inpatient ward. All interventions will be delivered within the Hospital Rehabilitation Department by hospital staff.


Patients who are admitted to the inpatient ward will be screened for eligibility as part of routine assessment. Suitable participants will be identified by the clinical team and given written information about the study. They will then be approached by a member of the research team to inquire whether they are interested and willing to take part in the study. Written consent will be obtained from participants who are willing to participate. A screening log of all nonrecruited patients and reasons for exclusion will be maintained.


Participants will be randomly allocated to the control or VR groups. A randomisation schedule will be pre-generated by the permuted block randomisation technique, using blocks of 10 participants with a 1:1 allocation ratio. The randomisation schedule will be calculated in SPSS by a statistical expert from the Faculty of Medical Statistics and Epidemiology, Sun Yat-sen University. The sequences of allocation are kept in a sealed envelope. The randomisation process will allocate each participant an identification number, which will appear on all report forms to maintain confidentiality.

Sample population

This study has the following inclusion criteria: (1) within 1 month of the first occurrence of stroke, (2) stage 2 of the Brunnstrom classification, (3) magnetic resonance imaging (MRI)- or computed tomography (CT)-confirmed stroke, (4) age between 40 and 80 years, (5) have at least 20° of wrist flexion/extension and at least 10° of finger flexion and extension of the affected limbs, (6) be able to walk at least 10 m with or without assistance and (7) no severe cognitive impairment (Mini Mental State Examination score below 10 [28]).

This study will exclude participants who are: (1) medically unstable, (2) have already received elements of the training programme over 1 week and (3) have brain stem injury.


The study was approved by the Medical Ethical Committee of the First Affiliated Hospital of Sun Yat-sen University (Ethics no.: [2014] 88). The clinical trial is registered with the Chinese Clinical Trial Registry (Registration No.: ChiCTR-IOC-15006064, registered on 11 March 2015). Any important protocol modifications will be communicated with all relevant parties. All patients who meet the criteria will be invited to take part in the study. Patients will be given time to consider whether they wish to take part in the trial and to ask any questions. All participants can withdraw from the trial at any time without giving reason. Participants can decide if they wish to withdraw completely, in which case all collected data will be excluded from the study, or to withdraw only from further assessment or intervention wherein all collected data will be included. All participants will be reimbursed for travel expenses to encourage adherence during the follow-up phase.

Outcome measures

Primary outcome measure

Gait speed, assessed by the 10-m Walk Test, is the primary outcome measure.

Secondary outcome measures

Secondary outcome measures include 3D motion analysis of upper limb reach forward and reach forward and grasp motions (Figs. 1 and 2) and gait. A breif description of each secondary outcome measure is presented in Table 1. Outcome measures will be recorded at baseline, post intervention and at 3, 6 and 12 months post intervention.

Fig. 1
figure 1

a Starting position of reach forward assessed by Vicon. b End position of reach forward motion

Fig. 2
figure 2

a Starting position of reach and grasp motion assessed by Vicon. b End position of each and grasp motion

Table 1 Descriptions of the secondary outcome measures

Outcome assessments

Outcome assessments will be conducted by the dedicated assessment team. The team consists of occupational therapists, physiotherapists, physicians and engineers who are specifically trained to perform the outcome assessments. The institute is a World Health Organisation Collaborating Centre and is responsible for delivering training on the use of the outcome measure tools to health care professionals.

Sample size

This study uses gait speed as the primary outcome measure for sample size calculation. An improvement in gait speed of 0.16 m/s was recommended as the minimum clinically significant difference during the first 60 days post stroke [29]. The sample size was determined by a statistical expert, using the formula:

$$ N=\frac{{\left({Z}_{\alpha}+{Z}_{\beta}\right)}^2{\sigma}^2}{\delta^2}\left({Q_1}^{-1}+{Q_2}^{-1}\right) $$

where Z α is normal distribution quantiles for type I error, Z β is normal distribution quantiles for type II error, σ is pooled standard deviation derived from past gait speed data, δ is difference in gait speed between the intervention and control group, and Q and Q are the proportions of control and intervention group.

Based on a 10% missing rate, each group requires 112 (total 224) participants to give 90% power to detect a gait speed difference of over 0.3 m/s.


The study procedure is illustrated in Fig. 3. The Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) figure shows the overview of the schedule of events (Fig. 4) (Additional file 1).

Fig. 3
figure 3

Flow diagram for the study procedure

Fig. 4
figure 4

Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) figure. Overview of the schedule of events

Control group

Participants in the control group will receive routine medical care and participate in conventional physiotherapy and occupational therapy. Therapeutic programmes include lower limb strengthening exercises and traditional gait and balance training, upper limb functional training and activities of daily living practice. The rehabilitation programme will be provided for 5 days a week for 3 weeks, with each therapy session lasting for 1 h (total of 2 h).

Intervention group

The intervention group will participate in a multisensory VR training programme for 5 days a week for 3 weeks. VR training is provided in addition to the routine programme. It consists of four components: gait training, limb coordination, gross motor function and fine motor function.

Gait training

Gait training will be provided through the use of the GaitWatch system (JumHo, China). The system requires the use of seven transmitters strapped to the following points: the lumbar spine at the L4/L5 level, the mid-portion of the femur, the tibial tuberosity and the mid-foot region (on both legs). Figure 4 shows the GaitWatch system and the locations of the transmitters (Fig. 5a, b). GaitWatch provides a gait training programme with bespoke gaming activities, which include marching on the spot, lifting hips and knees to required position or walking in a virtual environment (Fig. 5c, d). Real-time feedback on the quality of movement will be provided to the participants. Figure 4 shows the GaitWatch system and the locations of the transmitters. Training sessions will last up to 20 min (excluding a 5-min break at the halfway mark). The system will produce a report on gait speed, maximum joint angle achieved, weight distribution and percentage of deviation from expected motion trajectory.

Fig. 5
figure 5

GaitWatch system. a GaitWatch hardware. b Sensor position. c Real-time feedback displayed on the screen. d Walking tasks participants will be asked to complete

Limb coordination and gross upper limb motor function

Limb coordination and gross motor function will be trained through use of the Microsoft Xbox 360 Kinect via the games Adventures and Fruit Ninja. Participants will be asked to perform tasks that are displayed on the screen at progressive difficulties. The Adventures game will encourage balance, mobility and stepping. Fruit Ninja will encourage shoulder flexion/extension and internal/external rotation and elbow flexion and extension. Body motion is captured by the associated motion-capturing device. A body-weight support harness will be provided to those who are not able to stand for a sufficient length of time (Fig. 6). The score achieved during the virtual game will be recorded to monitor progress. Each training programme will last for 10 min (total of 20 min training time in addition to a 5-min break), 5 days a week for 3 weeks.

Fig. 6
figure 6

A body-weight support harness will be provided to those who are not able to stand for a sufficient length of time

Fine motor function

The fine motor function of the upper limbs will be trained using the HandTutor™ glove (Fig. 7) with MediTutor software (Meditouch Ltd., Rotem Industrial Park, MP Arava, Israel). The device delivers training via bespoke games. The gloves capture the biomechanical motions of the fingers and wrists. Exercises can be tailored to train all fingers or isolated finger movements. Real-time feedback of quality of movement is displayed on the screen. The previously published exercise programme ‘Track’ [30] will be used as the exercise for this study. ‘Track’ is a programme where a ball moves along a track horizontally across the screen at varying heights. The movement of the ball is controlled by flexion and extension of the finger/s or wrist. The participant’s task is to keep the ball within the track by flexing and extending the finger/s or wrist, which changes the upward and downward gradients of the ball. The therapist can set the participant’s range of motion that will be exercised by changing the height of the track. The maximum range of movement represents the full vertical displacement of the ball on the screen. The measurement is a linear measurement and treats the fingers as a single joint. Fine motor training will last for 20 min (with a 5-min break every 6 min), 5 days a week for 3 weeks.

Fig. 7
figure 7

HandTutor® glove for fine motor training

Safety and adverse event reporting

The participants recruited into this study are patients with acute stroke with a likelihood of morbidity and mortality. Procedures are, therefore, in place to minimise adverse events. The risk relating to the intervention is minimised as patients who are medically unstable will be excluded from the study. Participants will not be asked to exercise beyond their physical ability. The exercise sessions will be delivered by trained physiotherapists/occupational therapists who will work closely with the inpatient clinical team, and they will have regular contact and access to the inpatient clinical team for any queries that may arise. The trial will adhere to the following safety reporting procedures:

  1. 1.

    All serious adverse events (SAEs) related to the interventions will be recorded on an SAEs Event Report Form in accordance with local hospital procedure. A SAE is defined as an event that may harm the participant and may require medical or surgical intervention to prevent one of the following outcomes: death, a life-threatening situation, impairment of a body function, permanent damage to a body structure, and prolonged hospitalisation

  2. 2.

    All SAEs related to the interventions must be reported to the principle investigator within 24 h of learning of the event

  3. 3.

    SAEs that are related to the interventions will be reported to the special committee of the University Clinical Trial Unit and Research Ethics Committee within 15 days of the principle Investigator becoming aware of the event. Information that will be reported to relevant parties is: (1) appropriate identifying information for the research protocol, (2) a detailed description of the SAE and its outcome, (3) a description of any changes to the protocol or other corrective actions that have taken, or are proposed in response to the SAE

Data management

A bespoke data management system was developed by Rilintech Co., Ltd. (Beijing,China) for data storage. The assessment session and data entry are always conducted with at least two assessors to minimise data entry error. Monitoring of study conduct and data collection will be performed by a special committee of the University Clinical Trial Unit on a biannual basis. Recorded data are only accessible by authorised personnel.

Data analysis

Descriptive statistics will be used to describe the demographic data of each sample group. The normality of each parameter will be checked by histogram and the Kolmogorov-Smirnov test.

Chi-squared tests will be used to assess sample characteristics of gender and types of cerebral infarction.

The pre- and post-intervention differences in gait speed within individuals and between groups will be assessed by paired-samples t tests (two-tailed) and one-way analysis of variance (ANOVA), respectively. The comparison of 3-month, 6-month and 1-year follow-ups will be assessed by repeated-measures ANOVA.

A broadly similar approach will be used to analyse the secondary outcome measures of muscle tone, other kinematic parameters of gait, reach forward motion and reach forward and grasp motion. The kinematic parameters of the paretic side will be compared with the nonparetic side at all recording points using paired-samples t tests.

The Mann-Whitney U test will be used to test the between-group differences for the ordinal data obtained from the functional outcome measures and WHODAS 2.0.

The differences between the two groups during the follow-up phase will be assessed by the Kaplan-Meier test.

There are no planned interim analyses or stopping rules, as the power calculation has accounted for loss to follow-up. All analyses will initially be performed on an intention-to-treat basis.


The VR intervention works on the principle of neuroplasticity [31]. Studies from the past decade suggest that different types of VR can increase gait speed, lower and upper limb functions and kinematic parameters. There are a number of clinical guidelines which recommend active rehabilitation to be commenced within 24 to 48 h of stroke occurrence [32, 33] as the greatest part of the recovery was reported to take place in the first month following stroke [34]. There is some evidence to demonstrate the benefit of an early exercise programme on functional recovery but evidence for early VR intervention is lacking. The majority of the published studies focussed on patients with subacute and chronic stroke [16, 23]. Thus, this study will provide evidence for the use of VR as part of an early intervention programme.

The present study also assesses the medium- to long-term impact of VR interventions for patients with stroke during the acute stage. The Cochrane reviews published in 2011 and 2015 [6, 19] both concluded that it was unknown whether the effects of VR were sustained in the longer term. The review [35] found that only one study [36] out of the 37 included studies had measured the effects of VR on upper limb functions at the 6-month follow-up. Motor capacities evolve most strongly over the first month post stroke [37] and intervention provided at an early stage has better clinical outcomes when compared to intervention provided at a later stage of stroke. Thus, the follow-up data record at 3, 6 and 12 months post intervention will increase the understanding of whether early VR intervention is superior to conventional therapy in the longer term.

The kinematic data of gait and upper limb motion will help to establish if functional improvement induced by early VR intervention is associated with changes of kinematic patterns. Buma [38] proposed that the desired outcome of stroke rehabilitation was to promote task completion in a manner that is close to normal movement pattern. Detailed kinematic information would enable the identification of potential compensation movement. Nonbiomechanical outcome measures had in the past misled clinicians to believe that a faster observed speed of reach forward movement execution may improve movement quality as appreciated by the smoothness [39]. However, recent biomechanical study has revealed that the faster execution speed observed in patients with stroke was related to trunk compensation movement [40]. A kinematic study is, therefore, essential to assess whether VR intervention is effective in promoting normal movement patterns as well as functional improvement.

In conclusion, this current research is set to provide information to demonstrate the clinical effectiveness of VR as part of an early intervention programme for patients with stroke.

Trial status

Recruiting since May 2015.



Action Research Arm Test


Commercial gaming


Computed tomography


Mini Mental State Examination


Magnetic resonance imaging


Virtual reality


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We would like to thank all the clinical staff and participants for their contribution to the study.


This research project is supported by the Sun Yat-sen University Clinical Research 5010 funding programme (Grant No. 2014001). The publication of this protocol is supported by China Postdoctoral Science Foundation Grant (Grant No. 2016 M592581).

Availability of data and materials

Not applicable.

Authors’ contributions

All authors have read and approved the final manuscript. All authors meet the four primary ICMJE criteria for authorship. In addition, all authors have been, and are actively involved in, the study in different capacities: WL drafted the paper with contributions and approval from all authors. WL, YM, LL, DF, JZ, QL, HL and LC were involved in the development of study material, evaluation protocols and data collection. AL is the lead statistician. DF and YM manage the trial.

Authors’ information

Professor Dong Feng Huang is a physician. He is the Director of the Department of Rehabilitation Medicine and the Director of World Health Organisation Collaborating Centre for Rehabilitation of the First Affiliated Hospital, Sun Yat-sen University, China.

Dr. Wai Leung Ambrose Lo is a physiotherapist qualified in the United Kingdom. He is a research fellow at the Department of Rehabilitation Medicine, First Affiliated Hospital, Sun Yat-sen University, China.

Professor Yu Rong Mao is the Head Physiotherapist of the Department of Rehabilitation Medicine, First Affiliated Hospital, Sun Yat-sen University, China.

Professor Le Li is a human biomechanics scientist. He is the Associate Professor of the Department of Rehabilitation Medicine, First Affiliated Hospital, Sun Yat-sen University, China.

Professor Ai Hua Lin is a statistician in the Faculty of Medical Statistics and Epidemiology, School of Public Health, Sun Yat-sen University, China.

Ms. Jiang Li Zhao is a stroke specialist physiotherapist in the Department of Rehabilitation Medicine, First Affiliated Hospital, Sun Yat-sen University, China.

Dr. Qiang Lin is a physician. She is a PhD candidate of the Sun Yat-sen University, China.

Dr. Hai Li is a physician. She is a PhD candidate of the Sun Yat-sen University, China.

Dr. Ling Chen is a physician. She is a PhD candidate of the Sun Yat-sen University, China.

Competing interests

We declare that the authors have no competing interests. No commercial party has a direct financial interest in the results of the research, supported this paper, or will confer a benefit upon the authors or upon any organisation with which the authors are associated.

Consent for publication

Written informed consent was obtained from the participants for publication of their individual details and accompanying images in this manuscript. The Consent Form is held by the authors’ institution and is available for review by the Editor-in-Chief.

Ethics approval and consent to participate

The study was approved by the Medical Ethical Committee of the First Affiliated Hospital of Sun Yat-sen University (Ethics no.: [2014] 88). Written consent will be obtained from all patients who agree to take part in the study.


The results of this study will be presented at national and international conferences and published in a peer-reviewed journal. Participants can request a summary of the results.

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Correspondence to Wai Leung Ambrose Lo or Dong Feng Huang.

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SPIRIT 2013 Checklist: recommended items to address in a clinical trial protocol and related documents*. (DOCX 50 kb)

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Lo, W.L.A., Mao, Y.R., Li, L. et al. Prospective clinical study of rehabilitation interventions with multisensory interactive training in patients with cerebral infarction: study protocol for a randomised controlled trial. Trials 18, 173 (2017).

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