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Effectiveness of heat-sensitive moxibustion in the treatment of lumbar disc herniation: study protocol for a randomized controlled trial
© Chen et al; licensee BioMed Central Ltd. 2011
- Received: 10 January 2011
- Accepted: 13 October 2011
- Published: 13 October 2011
Lumbar disc herniation is a common and costly problem. Moxibustion is employed to relieve symptoms and might therefore act as a therapeutic alternative. Many studies have already reported encouraging results in heat-sensitive moxibustion for lumbar disc herniation. Hence, we designed a randomized controlled clinical trial to investigate the effectiveness of heat-sensitive moxibustion compared with conventional moxibustion.
This trial is a multicenter, prospective, randomized controlled clinical trial. The 316 eligible patients are randomly allocated to two different groups. The experimental group is treated with heat-sensitive moxibustion (n = 158); while the control group (n = 158) is treated with conventional moxibustion. The moxibustion locations are different for the groups. The experimental group selects heat-sensitization acupoints from the region which consists of bilateral Da Changshu (BL25) and Yao Shu (Du2). Meanwhile, fixed acupoints are used in control group; patients in both groups receive 18 sessions in 2 weeks.
The study design guarantees a high internal validity for the results. It is one large-scale randomized controlled trial to evaluate the efficacy of heat-sensitive moxibustion compared to conventional moxibustion and may provide evidence for this therapy as a treatment for moderate and severe lumbar disc herniation. Moreover, the result may uncover the inherent laws to improve the therapeutic effect with suspended moxibustion.
The trial is registered at Chinese Clinical Trials Registry: ChiCTR-TRC-09000604. The application date was 27 November 2009. The first patient was randomized on the 16 June 2011.
Lumbar disc herniation (LDH) is one of the most common causes of nerve root pain, and become a painful, debilitating disorder in working adults . In the majority of patients, experiencing their first episode of sciatica due to LDH, the symptoms recede to a non-disabling level within a period of six weeks .
LDH most typical symptoms are low back pain, accompanied by one or both lower extremity pain, numbness, pain, mostly by lumbosacral, buttocks, posterolateral to the lower limbs and feet, back radiation when bending, cough worse with movements. Herniated discs also often occur without symptoms, as revealed by magnetic resonance imaging studies in asymptomatic people. They are only clinically relevant when they impinge on a nerve root, causing radiculopathy [3, 4].
In general, LDH is due to disc degeneration or trauma leading to nucleus, annulus fibrosis to the vertebral canal prominent, spinal cord or nerve root compression. As a result, conventional treatments including physical therapy, pain medication, anti-inflammatory drugs and surgery, are provided by physicians. Most patients with low back pain respond well to conservative therapy [5, 6]. Recently, a large number of patients with LDH are turning to complementary and alternative treatments. Complementary and alternative treatments such as acupuncture are therefore attractive. Acupuncture is often used for LDH. For example, it is gaining popularity among LDH patients in the US and about 1 million consumers with musculoskeletal disorders utilize acupuncture annually .
Moxibustion is a traditional Chinese method that uses the heat generated by burning herbal preparations containing Artemisia vulgaris to stimulate acupoints . Moxibustion has anti-inflammatory or immunomodulatory effects against chronic inflammatory conditions in humans [9, 10]. Different moxibustion methods for treatment of LDH and their mechanism may be due to moxibustion can improve local blood circulation, eliminate nerve root inflammation and edema, loosen adhesions and improve protrusion and nerve root relations or the promotion of nerve injury repair [11, 12]. Moreover, the heat of moxa treatment improves microcirculation in the lumbar vertebra.
Therefore, these arthritis substances may be reduced and weakened by moxibustion. Especially for acute LDH, moxibustion may have a good effect. Severe low-back pain with leg pain (sciatica) may be caused by a herniated intervertebral disc exerting pressure on the nerve root in this stage. Nerve root inflammation and the surrounding edema aggravate the pain. The role of moxibustion may manage the pathological process.
There are many factors influencing therapeutic effect in moxibustion. But the selection of location for manipulating moxa should deserve the greatest attention . Our previous studies suggested that the dominating factor in selecting the location of acupoints is associated with the area on the body surface which is affected by disease, not only the standardized fixed position . In humans, acupoints contain two states: stimulated and resting. When people get sick, the acupoints on the body surface area are activated and sensitized. The sensitive areas are susceptible to heat stimulation and called "heat-sensitized acupoints". A feature of these areas is that they are specific or closely relevant to acupoints and produce the same clinical effect as "a small stimulation induces a large response". This sensitized acupoint is not only the pathological phenomenon reflecting the diseases but also an effective stimulating location with acupuncture and moxibustion .
Our research found that the heat-sensitive phenomenon to acupoint or an area is a new type of reaction in a pathological state [16–19]. A lot of observations and research were used to confirm this phenomenon in the 1990s . We summarized the experiential evidence and found that the state of acupoints might change from the rest state to the heat-sensitized state in patients when suffering from diseases. These special acupoints might bring out further heat sensation and response in the stimulation of moxibustion heat. If we can search out these heat-sensitized acupoints associating with pathological state, good effects may be achieved. Therefore, selecting the heat-sensitized acupoint may obtain therapeutic effect far better than acupuncture and moxibustion at acupoints of the routine resting state . So the heat-sensitive moxibustion is a medical technique usually involving suspended moxibustion on the heat-sensitized acupoint. A great many physicians utilized heat-sensitive moxibustion therapy in different kinds of diseases in China. Moreover, several articles and research have documented the effectiveness and safety of heat-sensitive moxibustion, such as myofascial pain syndrome , LDH , pressure sores  and knee osteoarthritis . Together these findings suggest a superiority effect of heat-sensitized acupoints. However, the results do not provide convincing evidence. This may be because of inappropriate sample size, variability of acupuncture and sham protocols, and missing information which were frequent methodological problems . Therefore, well-designed randomized controlled clinical trials are needed to establish the efficacy of heat-sensitive moxibustion for LDH.
The aim of this study is to investigate the effectiveness of heat-sensitive moxibustion compared with conventional moxibustion in patients with acute LDH in China.
List of M-JOA
Lumbar and leg pain
Mild pain or occasional moderate pain
Usual moderate pain or occasional severe pain
Frequent or continuous severe pain
Frequent but relief may be on their own
Continuous, not alleviated
Grade 0 to II
Straight let raising test
> 70°, straight leg drive up (-)
> 45°, straight leg drive up (+)
> 30°, straight leg drive up (+)
< 30°, straight leg drive up (+)
Activities of daily life and work
Stoop and lift heavy things
Normal stoop, lift weight > 3 kg
Able to stoop, lift weight ≤ 3 kg
Unable to stoop
Serious difficulties in bending and lifting
Walking ≥ 1 km or 60 min
Walking ≥ 500 m or 30 min
Walking ≥ 100 m or 10 min
Serious difficulties in walking
Daily time in bed(h)
10 to 12
12 to 16
Ability to work
Full-time to do as before
Although able to work, occasionally need a break
Although able to work, usually need a break
Unable to work
Therapeutic effect is assessed by comparing baseline and final conditions reported by the patient. This trial also records adverse effects reported by patients during treatment. The outcome measures above will be assessed before treatment, the 14 days after the last moxibustion session and 6 months after the last moxibustion session.
To evaluate the improvement of severity, we will make use of M-JOA score and take the secondary analysis. Improvement rate = [(scores before treatment - after treatment score)/pretreatment score] × 100%. Definition are listed below: Clinically important improvement as ≥ 75%, markedly effective improvement as 50 to 75%, improved as 30 to 50% and ineffective as < 30%. The numbers in the four categories will be calculated respectively at every time point.
Eligible participants will be those previously diagnosed with moderate to severe LDH, according to the M-JOA criteria(> 10 score). Patients will be required to complete the baseline LDH diary. Written informed consent will be obtained from each participant. Participants 18 to 65 years of age will be recruited from outpatient and inpatient in four centers. Standard of diagnosis listed as follows:
① Pain occurred in lower back and radiated to the lower limb; ② Limitations of tender point; ③ Straight leg raising test and it's strengthen test are positive; ④ Skin sensation, muscle strength and tendon reflex have some changes; Changes in spinal posture; ⑤ CT suggestive of disc herniation. The above standard mainly originated from the guiding principle of clinical research on new drugs (GPCRND) . Since the research involves moderate to severe LDH or severe LDH, the inclusion criteria will restrict the following conditions: According to the below LDH diagnosis standard, meanwhile, the triangle region formed with bilateral Da Changshu (BL 25)and Yao Shu (Du 2) of patients (Da Changshu- Yao Shu -contralateral Da Changshu intra-region) appear heat-sensitive points.
Participants will be instructed to stop LDH symptomatic relief medication during the run-in and treatment periods and will be provided the usual care instruction for LDH.
Figure 1 The flow diagram is intended to depict the passage of participants through this RCT.
Participants will be excluded if they suffer from serious life-threatening disease, such as disease of the heart and brain, blood, vessels, liver, kidney and hematopoietic system, and psychotic patients. Participants will not be eligible if they are female and whether pregnant or lactating. The following conditions
are also excluded items: appeared a single nerve palsy, or cauda equina nerve palsy, manifested as muscle paralysis or have rectum or bladder problems; complicated with lumbar spinal canal stenosis and space-occupying lesions or for other reasons; complicated with lumbar spine tumors, infections, tuberculosis; moxibustion syncope and unwilling to be treated with moxibustion; do not sign informed consent.
Moxibustion will be performed by certified acupuncture medical doctors at four centers. Qualified specialists of acupuncture in traditional Chinese medicine with at least five years of clinical experience will perform the acupuncture in this study. All treatment regimens will be standardized between four centers practitioners via video, hands-on training and internet workshops. Participants will be randomly assigned to the heat-sensitive moxibustion group, the conventional moxibustion group. In the former groups, a 22 mm (diameter) × 120 mm (length) moxa-sticks (Jiangxi Traditional Chinese Medicine Hospital, China) will be used. The patient is usually in the comfortable supine position for treatment, with 24°C to 30°C temperature in the room.
For the heat-sensitive moxibustion group, the moxa-sticks are lit by the therapist and held over the region consisting in two Da Changshu (BL 25) and Yao Shu (Du 2) of patients. The warming suspended moxibustion about distance of 3 cm are used to search for the acupoint heat-sensitization phenomenon. The following patient sensation will suggest the special heat-sensitization aupoint: diathermanous sensation due to moxa-heat, defined as the heat sensation conducting from the moxa local skin surface into deep tissue; expand heat sensation due to moxa-heat, defined as the heat sensation spreading to the surrounding little by little around the moxa point; transfer heat sensation due to moxa-heat, defined as the heat sensation transferring along some pathway or direction. When some acupoint exists one below sensation at least, therapists mark the point as a heat-sensitive acupoint. We try our best to seek all the special acupoints in each patient by the repeated assessment.
The therapists begin to treat patients from the most heat-sensitive intensity acupoint. Treatment sessions end when patients feel the acupoint heat-sensitization phenomenon has disappeared. Generally speaking, we select one point each time. One point is treated 45 minutes. Patients receive the treatment for two times daily in the first four days and for one time daily in remaining ten days. The full treatment contains 18 sessions over 14 days.
Conventional moxibustion group
Patients assigned to the conventional moxibustion group will receive fixed acupoint moxibustion. Common practices are similar with the first group. The different manipulation is that the therapists carry out warming moxibustion in traditional acupoint, selecting Da Changshu (BL 25), Wei Zhong (BL40) and A-shi Xue. One point is treated 15 minutes a time. The total time is 45 minutes as well. Three points are treated by suspended moxibustion at the same time. In the treatment, the therapists try to give patients the same intensity of the local warm sensation as the experimental group. The sensation of acupoint heat-sensitization phenomenon is not pursued and not avoided in the treatment. Patients receive the treatment for two times daily in the first four days and for one time daily in remaining ten days. The full treatment contains 18 sessions over 14 days.
Statistical analysis plan
We will conduct analysis on an intention to treat basis, including all randomized participants. Analyses will be conducted using 2-sided significance tests at the 5% significance level. The analysis of center effect will be used logistic regression method. The statistician conducting the analyses will remain blind to treatment group and data will only be unblinded once all data summaries and analyses are completed. All analyses will be conducted in the SAS statistical package program (ver. 9.1.3).
Baseline characteristics will be shown as mean, standard deviation (SD) for continuous data including age, previous duration, and so on. The first step of analysis is to check for integrity of randomization. The unbalanced analysis in baseline characteristics will use ANCOVA.
These will be summarized descriptively (mean, SD, median, minimum and maximum) at each time point by treatment group. The t-test, Mann-Whitney U and Wilcox on test were used for comparison of variables, as appropriate. When the data do not fit a normal distribution, we will consider more general tests such ANOVA and possible transformations.
All adverse events reported during the study will be included in the case report forms; the rates of adverse events will be calculated. The percentage of subjects with adverse events in each group will be calculated and compared using the chi-squared test or Fisher's exact test. The outcomes will be analyzed using linear mixed and logistic regression models that will include their respective baseline scores as covariates, subjects as a random effect and treatment conditions as fixed factors. Regression diagnostics will be used to check for normality of the measures and homogeneity of variance as appropriate.
Dropouts or missing data
Reasons for dropout or missing data will be explored descriptively. Multiple imputation will be used to make sure that dropouts do not impact the conclusions .
The primary outcome will be the M-JOA scores. The primary analysis will compare the two groups at 14 days. A secondary analysis will compare the two groups at 6 months to assess if any differences between groups have been maintained over time.
Loss to follow-up is likely to lead to biased estimates of intervention effect. We will try to avoid bias due to attrition by carefully following up the participants in both groups. We will phone participants who do not complete questionnaires after a second reminder. We anticipate a 20% loss to follow-up in this trial, and will implement procedures to minimize loss to follow-up and patient withdrawal, and where possible we will collect information on reasons for patient withdrawal.
The integrity of trial data will be monitored by regularly scrutinizing data sheets for omissions and errors. Data will be double-entered and the source of any inconsistencies will be explored and resolved.
We wished to estimate the sample size that would suffice to detect M-JOA between the heat-sensitive and conventional moxibustion groups. If we apply a two-sided 5% significance level, 90% power the calculated required sample size is approximately 126 participants in each group, according to the equations in . Allowing for a 20% loss to follow up, a total of 158 participants will be required in each group, with 316 participants in total.
We define adverse events as unfavorable or unintended signs, symptoms or disease occurring after treatment that are not necessarily related to the moxibustion intervention. In every visit, adverse events will be reported by participants and examined by the practitioner.
Written consent will be obtained from each participant. This study was approved by all relevant local ethics review boards. Ethics Committee of Affiliated hospital of Jiangxi Institute of Traditional Chinese Medicine had approved this trial: code issued by ethic committee is 2008(11).
Currently, although different therapeutic methods can be used in patients with LDH, the treatments can be divided into two categories, conservative (or non-surgical) and surgical. Conservative treatments mainly avoid painful positions and relieve symptoms in nine out of 10 people with a herniated disk, according to the American Academy of Orthopedic Surgeons . Many clinical trials held that moxibustion should be effective in the treatment of LDH. But the evidence obtained from these trials was quite limited because of methodology defects. Therefore, we designed this clinical trial to meet the CONSORT statement and guidelines to improve the chances of high internal validity for the results . On the basis of the classical notion of traditional Chinese medicine, moxibustion caused by the burning of moxa leads to the radiant heat and bring drug-like effects to acupoints. Practitioners use moxa to warm acupoints with the intention of stimulating circulation through the acupoints and inducing a smoother flow of blood and qi. So the selection of moxa locations has an important impact on obtaining good effects. It is widely believed that the acupoint locations are fixed along meridians. The conventional moxibustion applies moxa to get the desired results by stimulating these fixed acupoints. Doctors regard hyperemia due to local skin vasodilatation as the indicator of moxibustion effect. However, we support the notion that these fixed acupoints might not be the best stimulating sites for moxibustion.
In humans, acupoints contain two states: stimulated and resting. When people get sick, the acupoints on the body surface area are activated and sensitized. The sensitive areas are susceptible to heat stimulation and are called "heat-sensitized acupoints". Acupoints are more than fixed skin sites but externally sensitive reflecting diseases. Therefore, acupoint is variable and determined by the pathological state. Heat-sensitive moxibustion is a therapeutic method through heat-sensitized acupoints .
Acupuncture and moxibustion is a form of therapy derived from Traditional Chinese Medicine (TCM). It is well known that Huáng Dì Nèi Jīng (黄帝内经) is the source of theoretical and academic foundation in TCM. According to core theory in this classic,
pathological state of the internal diseases can be manifested through the acupoints. It is very difficult for persons to experience the existence of the acupoints in a normal physical state. However, patients can usually feel some changes in the area of the acupoints when they are sick.
With the state transition and change, sensitized status is often observed. Acupoints on the body surface may be sensitized with various types of sensitization under the circumstances. On the one hand, these sensitized acupoints are supposed to be the pathological phenomena reflecting the diseases; on the other hand, acupuncture and moxibustion treat diseases by stimulating these acupoints [14, 15]. Our clinical experience and research in the 1990s pointed out that acupoint
heat-sensitization was a type of acupoint sensitization. The features of acupoints heat-sensitization correspond with the classical thought and theory from the Inner Canon of Huangdi, according to the previous analysis in the above paragraphs . Therefore, it would be valuable to know whether heat-sensitized acupoints is superior to fixed points.
It is hypothesized that selecting the heat-sensitized acupoint may obtain therapeutic effect far better than moxibustion at acupoints of routine resting states. Our purpose is to test and verify the hypothesis. The study outcomes will facilitate the development of the theory of acupuncture and moxibustion. Therefore, the primary aim of this project is more than providing the efficacy of heat-sensitive moxibustion as a treatment modality in patients with LDH. The results of our trial will be helpful to disclose inherent law of moxibustion and present evidence for better therapeutic options to enhance the efficacy of moxibustion in China.
This study was supported by the Major State Basic Research Development Program of People's Republic of China (Grant number: 2009CB522902), the National Natural Science Foundation of China (Grant number: 30760320) and Jiangxi Key R&D Project.
- Keller RB, Atlas SJ, Singer DE, Chapin AM, Mooney NA, Patrick DL, Deyo RA: The Maine lumbar spine study, Part I. Background and concepts. Spine. 1996, 21 (15): 1769-76. 10.1097/00007632-199608010-00010.View ArticlePubMedGoogle Scholar
- Luijsterburg PA, Verhagen AP, Ostelo RW, van den Hoogen HJ, Peul WC, Avezaat CJ, Koes BW: Physical therapy plus general practitioners' care versus general practitioners' care alone for sciatica: a randomized clinical trial with a 12-month follow-up. Eur Spine J. 2008, 17 (4): 509-517. 10.1007/s00586-007-0569-6.View ArticlePubMedPubMed CentralGoogle Scholar
- Gibson JNA, Waddell G: Surgical interventions for lumbar disc prolapsed. Cochrane Database of Systematic Reviews. 2007, 2Google Scholar
- Andersson GB, Brown MD, Dvorak J, Herzog RJ, Kambin P, Malter A, McCulloch JA, Saal JA, Spratt KF, Weinstein JN: Consensus summary of the diagnosis and treatment of lumbar disc herniation. Spine. 1996, 21 (24 Suppl): S75-78.View ArticleGoogle Scholar
- Peul WC, van Houwelingen HC, van den Hout WB, Brand R, Eekhof JA, Tans JT, Thomeer RT, Koes BW, Leiden-The Hague Spine Intervention Prognostic Study Group: Surgery versus prolonged conservative treatment for sciatica. N Engl J Med. 2007, 356 (22): 2245-2256. 10.1056/NEJMoa064039.View ArticlePubMedGoogle Scholar
- Chae KH, Ju CI, Lee SM, Kim BW, Kim SY, Kim HS: Strategies for Noncontained Lumbar Disc Herniation by an Endoscopic Approach: Transformational Suprapedicular Approach, Semi-Rigid Flexible Curved Probe, and 3-Dimensional Reconstruction CT with Discogram. J Korean Neurosurg Soc. 2009, 46 (4): 312-316. 10.3340/jkns.2009.46.4.312.View ArticlePubMedPubMed CentralGoogle Scholar
- Paramore LC: Use of alternative therapies: estimates from the 1994 Robert Wood Johnson Foundation National Access to Care Survey. J Pain Symptom Manage. 1997, 13 (2): 83-89. 10.1016/S0885-3924(96)00299-0.View ArticlePubMedGoogle Scholar
- Lee DH, Kim JI, Lee MS, Choi TY, Choi SM, Ernst E: Moxibustion for ulcerative colitis: a systematic review and meta-analysis. BMC Gastroenterol. 2010, 10: 36-10.1186/1471-230X-10-36.View ArticlePubMedPubMed CentralGoogle Scholar
- Cho ZH, Hwang SC, Wong EK, Son YD, Kang CK, Park TS, Bai SJ, Kim YB, Lee YB, Sung KK, Lee BH, Shepp LA, Min KT: Neural substrates, experimental evidences and functional hypothesis of acupuncture mechanisms. Acta Neurol Scand. 2006, 113 (6): 370-377. 10.1111/j.1600-0404.2006.00600.x.View ArticlePubMedGoogle Scholar
- Chen W, Yang AT, Dai MT, Fu QL: [Observation on therapeutic effect of electroacupuncture under continuous traction for treatment of lumbar disc herniation]. Zhongguo Zhen Jiu. 2009, 29 (12): 967-9. [Chinese]PubMedGoogle Scholar
- Chen HL, Qiu XH, Yan XC: [Observation on therapeutic effect of electroacupuncture plus blood-letting puncture at Weizhong (BL 40) on acute lumbar disc herniation]. Zhongguo Zhen Jiu. 2009, 29 (2): 123-5. [Chinese]PubMedGoogle Scholar
- Peng KZ, Xiang KW, Cui J: [Observation on therapeutic effect of intradermal needle combined with Tuina on lumbar disc herniation]. Zhongguo Zhen Jiu. 2008, 28 (12): 894-6. [Chinese]PubMedGoogle Scholar
- Ewies A, Olah K: Moxibustion in Breech Version-A Descriptive Review. Acupuncture in medicine. 2002, 20 (1): 26-29. 10.1136/aim.20.1.26.View ArticlePubMedGoogle Scholar
- Chen RX, Chen MR, Xiong J, Yi F, Chi ZH, Zhang B: Comparison of heat-sensitive moxibustion versus fluticasone/salmeterol (seretide) combination in the treatment of chronic persistent asthma: design of a multicenter randomized controlled trial. Trials. 2010, 11: 121-10.1186/1745-6215-11-121.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen RX, Chen MR, Kang MF, Xiong J, Chi ZH, Zhang B, Fu Y: The design and protocol of heat-sensitive moxibustion for knee osteoarthritis: a multicenter randomized controlled trial on the rules of selecting moxibustion location. BMC Complement Altern Med. 2010, 10: 32-10.1186/1472-6882-10-32.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen RX, Kang MF: [Clinical application of acupoint heat-sensitization]. Zhongguo Zhen Jiu. 2007, 27 (3): 199-202. [Chinese]PubMedGoogle Scholar
- Chen RX, Kang MF: [Key point of moxibustion, arrival of qi produces curative effect]. Zhongguo Zhen Jiu. 2008, 28 (1): 44-46. [Chinese]PubMedGoogle Scholar
- Chen RX, Kang MF, He WL, Chen SY, Zhang B: [Moxibustion on heat-sensitive acupoints for treatment of myofascial pain syndrome: a multi-central randomized controlled trial]. Zhongguo Zhen Jiu. 2008, 28 (6): 395-398. [Chinese]PubMedGoogle Scholar
- Chen RX, Kang MF: Acupoint heat-sensitization moxibustion: a new therapy. 2006, Press by People's Medical Publishing House. Beijing in China, 1Google Scholar
- Tang FY, Huang CJ, Chen RX, Xu M, Liu BX, Liang Z: [Observation on therapeutic effect of moxibustion on temperature-sensitive points for lumbar disc herniation]. Zhongguo Zhen Jiu. 2009, 29 (5): 382-384. [Chinese]PubMedGoogle Scholar
- Zhang C, Xiao H, Chen Rx: [Observation on curative effect of moxibusting on heat-sensitive points on pressure sores]. China Journal of Traditional Chinese. Medicine and Pharmacy. 2010, 25 (4): 478-488. [Chinese]Google Scholar
- Kang MF, Chen RX, Fu Y: [Clinical Research on Ostioarthritis of Knee Treated with Moxibustion to Heat Sensitivity Point]. Journal of Jiangxi university of TCM. 2006, 18 (2): 27-28. [Chinese]Google Scholar
- Fukui M, Chiba K, Kawakami M, Kikuchi S, Konno S, Miyamoto M, Seichi A, Shimamura T, Shirado O, Taguchi T, Takahashi K, Takeshita K, Tani T, Toyama Y, Wada E, Yonenobu K, Tanaka T, Hirota Y: JOA back pain evaluation questionnaire: initial report. J Orthop Sci. 2007, 12 (5): 443-50. 10.1007/s00776-007-1162-x.View ArticlePubMedPubMed CentralGoogle Scholar
- Liu B, Wen T, Yao C, Yan S, He L, Xie Y, Mu Z: [The central randomization system in multi-center clinical trials]. Chin J New Drugs Clin Rem. 2006, 12 (2): 931-935. [Chinese]Google Scholar
- Wayman JC: Multiple Imputation For Missing Data: What Is It And How Can I Use It?. Accessed on August 1 2011, http://www.csos.jhu.edu/contact/staff/jwayman_pub/wayman_multimp_aera2003.pdf
- Wu SX, Wang CX: Clinical studies estimate sample size. 2008, Press by People's Medical Publishing House. Beijing in China, 1Google Scholar
- Snelling NJ: Spinal manipulation in patients with disc herniation: A critical review of risk and benefit. International Journal of Osteopathic Medicine. 2006, 9 (3): 77-84. 10.1016/j.ijosm.2006.08.001.View ArticleGoogle Scholar
- Moher D, Hopewell S, Schulz KF, Montori V, Gøtzsche PC, Devereaux PJ, Elbourne D, Egger M, Altman DG, Consolidated Standards of Reporting Trials Group: CONSORT 2010 Explanation and Elaboration: updated guidelines for reporting parallel group randomized trials. BMJ. 2010, 63 (8): e1-37.Google Scholar
- Veith I, translator (1972): The Yellow Emperor's Classic of Internal Medicine. Berkeley, CA: University of California Press, Revised paperbackGoogle Scholar
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