Variables | Measuring methods | Baseline (2022.9) | 3Â months after baseline (2022.12) | 9Â months after baseline (2023.6) | 24Â months after baseline (2024.9) | 36Â months after baseline (2025.9) |
---|---|---|---|---|---|---|
Anthropometric measures | ||||||
 Height | Stadiometer | √ | √ | √ | √ | √ |
 Weight | Lever scale | √ | √ | √ | √ | √ |
 Waist circumference | Tape | √ | √ | √ | √ | √ |
 Hip circumference | Tape | √ | √ | √ |  |  |
 Blood pressures | Digital sphygmomanometer | √ | √ | √ |  |  |
 Body composition | Inbody770 (Biospace, USA) | √ | √ | √ |  |  |
Blood measures | ||||||
 Fasting glucose | Follow clinical standard protocols | √ |  | √ |  |  |
 Fasting insulin | √ |  | √ |  |  | |
 Triacylglycerol | √ |  | √ |  |  | |
 Total cholesterol | √ |  | √ |  |  | |
 Low-density lipoprotein cholesterol | √ |  | √ |  |  | |
 High-density lipoprotein cholesterol | √ |  | √ |  |  | |
 Alanine aminotransferase | √ |  | √ | √ | √ | |
Liver ultrasound measures | ||||||
 Liver stiffness measurement | FibroScan HANDY (Echosense, France) | √ | √ | √ | √ | √ |
 Controlled attenuation parameters | FibroScan HANDY (Echosense, France) | √ | √ | √ | √ | √ |
Physical fitness measure | ||||||
 20-m shuttle-run test | Conducted by trained physical health teacher | √ |  | √ | √ | √ |
Behavioural measures | ||||||
 Students’ eating behaviour | Dietary quality questionnaire (DQQ) [20] | √ |  | √ |  |  |
 Students’ physical activity behaviour | Self-designed questionnaire [18] | √ |  | √ |  |  |
 Psychological status | Center for Epidemiologic Studies Depression Scale (CES-D Scale) [21] and Social Anxiety Scale for children (SASC) [22] | √ |  | √ |  |  |
 Sleep quality | Pittsburgh sleep quality index (PSQI) [23] | √ |  | √ |  |  |