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Anti-COVID property of subcutaneous ivermectin in synergy with zinc among midlife moderately symptomatic patients: a structured summary of a study protocol for a randomised controlled trial
Trials volume 22, Article number: 591 (2021)
The study objective is to quantify the effectiveness of ivermectin (subcutaneous/oral IVM) in the presence or absence of zinc (Zn) for clinical and radiological improvement in coronavirus disease 2019 (COVID-19) patients with moderate severity.
This quadruple-blinded, placebo-controlled randomized clinical trial will be a multiarmed multi-centered study with superiority framework.
Quinquagenarian and sexagenarian patients with moderate COVID-19 symptoms and positive severe respiratory syndrome coronavirus -2 (SARS-CoV-2) PCR will be included. Participants with co-morbidities and pregnant women will be excluded.
Patient recruitment will be done in Shaikh Zayed Medical Complex, Doctors Lounge and Ali Clinic in Lahore (Pakistan).
Intervention and comparator
The registered patients will be allocated in 6 groups (30 participants each). Patients will be taking subcutaneous IVM at 200 μg/kg/48 h (Arm A) or subcutaneous IVM at 200 μg/kg/48 h and oral Zn 20mg/8 h (Arm B) or oral IVM at 0.2 mg/kg/day (Arm C) or oral IVM at 0.2 mg/kg/day and oral Zn 20mg/8 h (Arm D) or alone oral Zn 20mg/8 h (Arm E) or placebo alone (Arm X). Patients in all arms will receive standard care and respective placebo (empty capsule 8 hourly and/or subcutaneous normal saline 2ml/48 h).
Primary endpoints will be duration of symptomatic phase and SARS-CoV-2 clearance along with high resolution CT (HRCT) chest score and clinical grade scale (CGS) on day 6. 30-day mortality will be documented as a secondary endpoint. SARS-CoV-2 clearance will be calculated by second PCR on day 7. HRCT chest score will be measured by the percentage and lung lobes involvement on day 6 with a maximum score of 25. CGS will be recorded on a seven-point scale; grade 1 (not hospitalized, no evidence of infection and resumption of normal activities), grade 2 (not hospitalized, but unable to resume normal activities), grade 3 (hospitalized, not requiring supplemental oxygen), grade 4 (hospitalized, requiring supplemental oxygen), grade 5 (hospitalized, requiring nasal high-flow oxygen therapy and/or noninvasive mechanical ventilation), grade 6 (hospitalized, requiring ECMO and/or invasive mechanical ventilation) and grade 7 (death).
A simple lottery method will be used to randomly allocate scrutinized patients in 1:1:1:1:1:1 ratio in 6 groups.
Patients, primary care physicians, outcome assessors and the data collection team will be blinded.
Numbers to be randomised (sample size)
180 participants will be randomized into six arms with five investigational and one placebo group.
Institutional Review Board Shaikh Zayed Post-Graduate Medical Complex, Lahore, Pakistan has approved the protocol (version 2.3) with ID SZMC/IRB/Internal0056/2020. The trial was approved on July 14, 2020, and enrolment started on July 30, 2020. The estimated completion date is October 30, 2021.
Clinical Trial has been retrospectively registered on www.clinicaltrials.gov with registration ID NCT04472585 dated July 16, 2020.
The full protocol is attached as an additional file, accessible from the Trials website (Additional file 1). With the intention of expediting dissemination of this trial, the conventional formatting has been eliminated; this Letter serves as a summary of the key elements of the full protocol. The study protocol has been reported in accordance with the Standard Protocol Items: Recommendations for Clinical Interventional Trials (SPIRIT) guidelines.
Availability of data and materials
Dr. Sohaib Ashraf will have access to the final trial dataset, and this could be available from the author on reasonable request, but the dataset is subject to data protection regulations. (Email address: email@example.com Mobile Number: +1 (857) 316 7995)
We would like to thank and acknowledge the efforts of Prof. Dr. Zia Ullah (Professor of Internal Medicine, Shaikh Zayed Post-Graduate Medical Complex, Lahore, Pakistan) who lost his life in the battle against COVID-19. We are particularly grateful for the help of our local and international collaborator group, DOCTORS LOUNGE Consortium.
DOCTORS LOUNGE Consortium
Abubakr Hilal1, Arz Muhammad1, Zeeshan Shaukat1, Sohail Ahmad2, Kanwal Hayat1, Ghazala Amjad1, Misbah Kousar1, Hadiqa Tul Hafsa1, Zawar Ahmad Choudhary1, Umair Hafeez1, Tayyab Mughal1, Noman Khalid1, Qurrat-ul-Ain1, Roa Umer1, Tayyaba Muzafar1, Sibgha Zulfiqar1, Saadia Shahzad Alam1, Saulat Sarfraz1, Muhammad Imran Anwar1, Amber Malik1,Talha Mahmud1, Adeen Akmal2, Muhammad Faisal Nadeem2, Nazish Matti2, Muhammad Azam2, Nighat Majeed3, Ali Arshad4, Khawar Nawaz5, Muhammad Ismail Khalid Yousaf6, Aadil Maqsood7 Atif Amin Baig8, Muhammad Bilal9, Muhammad Nauman Zahid10
Affiliations: 1, Shaikh Zayed Post-Graduate Medical Complex, Lahore, Pakistan. 2, University of Veterinary and Animal Sciences, Lahore, Pakistan. 3, Services Institute of Medical Sciences, Lahore, Pakistan. 4, Kingedward Medical University, Lahore, Pakistan. 5, Sunny Downstate/Kings Country Medical Center, New York, USA. 6. University of Louisville, Kentucky, USA. 7, University of Toledo Medical Center, Ohio, USA. 8. University Sultan Zainal Abidin, Malaysia. 9. McGill University, Canada. 10. University of Bahrain, Bahrain.
It is an investigator-initiated human clinical trial and funding was achieved from Smile Welfare Organization. Authors declare no role of the funding body in the design of the study, in the collection, analysis, and interpretation of data, and in writing the manuscript.
Ethics approval and consent to participate
Ethics Committee of Shaikh Zayed Post-Graduate Medical Complex, Lahore, Pakistan has approved the study on July 14th, 2020 with ID SZMC/IRB/Internal0056/2020. I certify that this trial has received ethical approval from the appropriate ethical committee as described above. Prior to enrolment, participants will be fully informed of the study and asked to sign the consent form in order to be eligible for randomization and participation.
Consent for publication
The authors declare that they have no competing interests.
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Ashraf, S., Ashraf, S., Farooq, I. et al. Anti-COVID property of subcutaneous ivermectin in synergy with zinc among midlife moderately symptomatic patients: a structured summary of a study protocol for a randomised controlled trial. Trials 22, 591 (2021). https://doi.org/10.1186/s13063-021-05487-z
- Subcutaneous Ivermectin
- Randomised controlled trial