Fig. 1

Ceftriaxone increases CD73 on T lymphocytes, and CD73 levels correlate with IFN-γ. A T cells were purified from peripheral blood from pre-operative coronary artery bypass patients and incubated in the absence (control) or presence of 50 μg/ml ceftriaxone for 24 h. B CD73 was measured in viable CD3 T cells gated as shown (red gate). C Quantification of flow cytometric data showing CD73 in total CD3 T cells and subpopulations of CD4+ and CD8+ unpaired t test, n=4. D Subpopulations of peripheral blood cells. E CD3+ T lymphocytes were gated and subpopulations of CD4+ (blue gate) and CD8+ (red gate) identified (F). G, H The expression of CD73 and production of IFN-γ in subpopulations of CD4+ (blue gate) and CD8+ (red gate) T lymphocytes