Table 2 Overview of parameters assessed in the planned study

Clinical examinations

 Disease history of children and parents/guardians

 Subjective health complaints (15 items)

 Blood pressure (SBP, DBP)

Blood testing

 Haemoglobin concentration (Hb)

 Blood lipids (TC, HDL-C, LDL-C, TG, Non-HDL, C-HDL ratio)

 Blood glucose (HbA1c)

 Micronutrient status (vitamin A, vitamin D, zinc, transferrin)

 Cytokines (IL-6)

 Leptin

Anthropometric measurements

 Body weight and height

 Body composition (body fat)

 Waist-to-hip ratio

 Body mass index

Parasitological examinations

 Soil-transmitted helminths (Ascaris lumbricoides, hookworm, Trichuris trichiura)

 Schistosoma mansoni

Cognitive function and academic performance

 Flanker task (executive function)

 School grades

Student survey

 Socio-demographic background (sex, ethnicity, home language)

 Self-reported physical activity

 Health-related quality of life (KIDSCREEN-10)

 Perceived stress (1 item)

 School satisfaction (1 item)

 Perceived academic competence (1 item)

 Sleep (6 items)

Objective assessment of physical activity

 7-day actigraphy

Fitness testing

 20-m shuttle run test (cardiorespiratory fitness)

 Grip strength test (upper body strength)

Parental survey

 Family socioeconomic status

 Dietary intake information (food frequency questionnaire)

 Food insecurity

 Sleep

Other variables

 Country, school, grade, class, distance/traveling to/from school