Table 2 Overview of the measurements and methodology at baseline and post-test in the HEPAFIT study

 Hepatic fat (%)

Vibration-controlled transient elastograph

 Body mass (kg)

Scale

 Height (cm)

Stadiometer

 Waist circumference (cm)

Non-elastic tape

 Blood pressure

Oscillometric monitor device

 Heart rate

Oscillometric monitor device

 Lean mass (kg), total body fat (%), bone mineral density (g/cm²), abdominal adiposity (g), truncal adiposity (g), limb adiposity (g)

Dual X-ray absorptiometry

 Visceral adiposity (cm²)

Bioelectrical impedance

 High-density lipoprotein cholesterol (mg/dL), low-density lipoprotein cholesterol (mg/dL), total cholesterol (mg/dL), total triglycerides (mg/dL), serum glucose (mg/dL)

Enzymatic spectrophotometry

 Serum insulin (mU/L)

Electrochemiluminescence immunoassay

 Glycohemoglobin (mg/dL)

Electrochemiluminescence immunoassay

 Alanine (U/L), aspartate (U/L), gamma-glutamyl transferase (U/L)

Enzymatic assay

 Uric acid, albumin (mg/dL), ferritin (mg/dL)

Enzymatic assay

 hs-C reactive protein, ENA-78, GCSF, GM-CSF, GRO, GRO-α, I-309, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-γ, MCP-1, MCP-2, MCP-3, MCSF, MDC, MIG, MIP-1β, MIP-1δ, RANTES, SCF, SDF-1, TARC, TGF-β1, TNF-α, TNF-β, EGF, IGF-I, angiogenin, oncostatin M, thrombopoietin, VEGF-A, PDGF-BB, leptin, BLC, Ckß8–1, eotaxin, eotaxin-2, eotaxin-3, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 ligand, fractalkine, GCP-2, GDNF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-16, IP-10, LIF, LIGHT, MCP-4, MIF, MIP-3α, NAP-2, osteopontin, osteoprotegerin, PARC, PLGF, TGF-β2, TGF-β3, TIMP-1, TIMP-2 (mean spot pixel densities)

Proteome Profiler human cytokine array panel A kit

 NT-3, NT-4, BDNF (pg/mL)

Surface plasmon resonance biosensors

 Flow-mediated dilation

High-resolution ultrasound device

 Carotid intima-media thickness

High-resolution ultrasound device

 Cardiorespiratory fitness

20-m shuttle run test

Cardiopulmonary exercise test

 Muscular strength

Handgrip strength

Standing long jump tests

 Speed and agility

4 × 10 shuttle run test

 Flexibility

Sit-and-reach test

 Attention capacity

d2 attention test

Stroop task test

 Physical activity enjoyment

Physical activity enjoyment scale

 Physical activity and sedentary behavior

Actigraph GT1M, dual-axis accelerometer

ActivPal

 Dietary assessment

24-h diet record

Food frequency questionnaires

 Pubertal development (Tanner stage)

Questionnaire

 Family medical history, demographic characteristics, socioeconomic status

Questionnaire