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Table 1 Primary and secondary outcome measures and other entomological indices

From: Assessing dengue transmission risk and a vector control intervention using entomological and immunological indices in Thailand: study protocol for a cluster-randomized controlled trial

Outcome No. Index abbreviation Index name Description Unit Frequency of data collection Details
Primary outcome 1 AI Adult index Number of female adult Ae. aegypti and Ae. albopictus per house collected both indoors and outdoors No./house Once every 4 months in all households (HHs) and once every month in 3 HHs per cluster (the same ones each time) Adult collections using a mechanical battery-powered aspirator for 30 min per house (indoors and outdoors)
Secondary outcomes 2 DIR Dengue incidence rate Number of confirmed dengue cases/observation days of household populations Rate Weekly VHVs detect fever cases. Hospital and project staff collect blood samples
3 MEI Mosquito exposure index (1) Differential optical density for antibodies to Ae. aegypti saliva (2) Proportion above the immune threshold for this assay (1) Number (2) % Once every 4 months in all HHs and once every month in 3 HHs per cluster (the same HH as for the AI) Recurring blood spots from two persons per HH taken on filter paper. IgG antibody response (positive or negative) to the Ae. aegypti Nterm-34 kDa salivary peptide
4 IAI Infected adult index Number of DENV-infected adult female Ae. aegypti and Ae. albopictus No./house Once every 4 months in all HHs and once every month in 3 HHs per cluster Based on adult mosquito collections indoors using a mechanical battery-powered aspirator for 15 min per house and DENV detection in individual mosquitoes
5 ASTI Adult sticky trap index Total number of Ae. aegypti and Ae. albopictus females collected by sticky traps per month No./trap/month 7 consecutive days per month Adult mosquitoes collected by sticky traps baited with hay infusion for 7 days every month
6 PPI Pupae per person index Number of Aedes pupae per person No./person Once every 4 months in all HHs and once every month in 3 HHs per cluster From immature collections. All pupae collected divided by the number of household participants
7 BI Breteau index Number of Aedes positive containers per 100 houses No./100 houses Once every 4 months in all HHs and once every month in 3 HHs per cluster From immature collections. Cluster-level result
Stegomyia indices 8 HI House index Proportion of houses positive for immature Aedes % Once every 4 months in all HHs and once every month in 3 HHs per cluster From immature collections. Cluster-level result
9 CI Container index Proportion of containers positive for immature Aedes % Once every 4 months in all HHs and once every month in 3 HHs per cluster From immature collections. Cluster-level result
Pupal indices 10 IPPI Infected pupae per person index Number of DENV infected Aedes pupae per person No./person Once every 4 months in all HHs and once every month in 3 HHs per cluster Based on PPI and DENV detection. All infected pupae collected divided by the number of household participants
11 PHI Pupae per house index Number of pupae per house No./house Once every 4 months in all HHs and once every month in 3 HHs per cluster Pupal collections
12 IPHI Infected pupae per house index Number of DENV-infected Aedes pupae per house No./house Once every 4 months in all HHs and once every month in 3 HHs per cluster Pupal collections and DENV detection
Adult indices 13 AII Adult indoor index Number of Ae. aegypti and Ae. albopictus females per house indoors No./house Once every 4 months in all HHs and once every month in 3 HHs per cluster Adult collections indoors using a mechanical battery-powered aspirator for 15 min per house
14 IAII Infected adult indoor index Number of DENV-infected Ae. aegypti and Ae. albopictus females per house indoors No./house Once every 4 months in all HHs and once every month in 3 HHs per cluster Based on AII and DENV detection
15 AOI Adult outdoor index Number of Ae. aegypti and Ae. albopictus females per house outdoors No./house Once every 4 months in all HHs and once every month in 3 HHs per cluster Adult collections outdoors using a mechanical battery-powered aspirator for 15 min per house
16 IAOI Infected adult outdoor index Number of DENV-infected Ae. aegypti and Ae. albopictus females per house outdoors No./house Once every 4 months in all HHs and once every month in 3 HHs per cluster Based on AOI and DENV detection
17 IASTI Infected adult sticky trap index Number of DENV-infected Ae. aegypti and Ae. albopictus females per sticky trap No./trap/week Once every 4 months in all HHs and once every month in 3 HHs per cluster Based on ASTI and DENV detection
Premise index 18 PCI Premise condition index Degree of shade + condition of house + condition of yard Number (min = 3, max 9) Once every 4 months in all HHs Observation criteria [90]
  1. VHV village health volunteer, IgG immunoglobulin G