Table 1 Primary and secondary outcome measures and other entomological indices
Outcome | No. | Index abbreviation | Index name | Description | Unit | Frequency of data collection | Details |
|---|---|---|---|---|---|---|---|
Primary outcome | 1 | AI | Adult index | Number of female adult Ae. aegypti and Ae. albopictus per house collected both indoors and outdoors | No./house | Once every 4 months in all households (HHs) and once every month in 3 HHs per cluster (the same ones each time) | Adult collections using a mechanical battery-powered aspirator for 30 min per house (indoors and outdoors) |
Secondary outcomes | 2 | DIR | Dengue incidence rate | Number of confirmed dengue cases/observation days of household populations | Rate | Weekly | VHVs detect fever cases. Hospital and project staff collect blood samples |
3 | MEI | Mosquito exposure index | (1) Differential optical density for antibodies to Ae. aegypti saliva (2) Proportion above the immune threshold for this assay | (1) Number (2) % | Once every 4 months in all HHs and once every month in 3 HHs per cluster (the same HH as for the AI) | Recurring blood spots from two persons per HH taken on filter paper. IgG antibody response (positive or negative) to the Ae. aegypti Nterm-34 kDa salivary peptide | |
4 | IAI | Infected adult index | Number of DENV-infected adult female Ae. aegypti and Ae. albopictus | No./house | Once every 4 months in all HHs and once every month in 3 HHs per cluster | Based on adult mosquito collections indoors using a mechanical battery-powered aspirator for 15 min per house and DENV detection in individual mosquitoes | |
5 | ASTI | Adult sticky trap index | Total number of Ae. aegypti and Ae. albopictus females collected by sticky traps per month | No./trap/month | 7 consecutive days per month | Adult mosquitoes collected by sticky traps baited with hay infusion for 7 days every month | |
6 | PPI | Pupae per person index | Number of Aedes pupae per person | No./person | Once every 4 months in all HHs and once every month in 3 HHs per cluster | From immature collections. All pupae collected divided by the number of household participants | |
7 | BI | Breteau index | Number of Aedes positive containers per 100 houses | No./100 houses | Once every 4 months in all HHs and once every month in 3 HHs per cluster | From immature collections. Cluster-level result | |
Stegomyia indices | 8 | HI | House index | Proportion of houses positive for immature Aedes | % | Once every 4 months in all HHs and once every month in 3 HHs per cluster | From immature collections. Cluster-level result |
9 | CI | Container index | Proportion of containers positive for immature Aedes | % | Once every 4 months in all HHs and once every month in 3 HHs per cluster | From immature collections. Cluster-level result | |
Pupal indices | 10 | IPPI | Infected pupae per person index | Number of DENV infected Aedes pupae per person | No./person | Once every 4 months in all HHs and once every month in 3 HHs per cluster | Based on PPI and DENV detection. All infected pupae collected divided by the number of household participants |
11 | PHI | Pupae per house index | Number of pupae per house | No./house | Once every 4 months in all HHs and once every month in 3 HHs per cluster | Pupal collections | |
12 | IPHI | Infected pupae per house index | Number of DENV-infected Aedes pupae per house | No./house | Once every 4 months in all HHs and once every month in 3 HHs per cluster | Pupal collections and DENV detection | |
Adult indices | 13 | AII | Adult indoor index | Number of Ae. aegypti and Ae. albopictus females per house indoors | No./house | Once every 4 months in all HHs and once every month in 3 HHs per cluster | Adult collections indoors using a mechanical battery-powered aspirator for 15 min per house |
14 | IAII | Infected adult indoor index | Number of DENV-infected Ae. aegypti and Ae. albopictus females per house indoors | No./house | Once every 4 months in all HHs and once every month in 3 HHs per cluster | Based on AII and DENV detection | |
15 | AOI | Adult outdoor index | Number of Ae. aegypti and Ae. albopictus females per house outdoors | No./house | Once every 4 months in all HHs and once every month in 3 HHs per cluster | Adult collections outdoors using a mechanical battery-powered aspirator for 15 min per house | |
16 | IAOI | Infected adult outdoor index | Number of DENV-infected Ae. aegypti and Ae. albopictus females per house outdoors | No./house | Once every 4 months in all HHs and once every month in 3 HHs per cluster | Based on AOI and DENV detection | |
17 | IASTI | Infected adult sticky trap index | Number of DENV-infected Ae. aegypti and Ae. albopictus females per sticky trap | No./trap/week | Once every 4 months in all HHs and once every month in 3 HHs per cluster | Based on ASTI and DENV detection | |
Premise index | 18 | PCI | Premise condition index | Degree of shade + condition of house + condition of yard | Number (min = 3, max 9) | Once every 4 months in all HHs | Observation criteria [90] |