Lifestyle intervention in children with obesity and nonalcoholic fatty liver disease (NAFLD): study protocol for a randomized controlled trial in Ningbo city (the SCIENT study)

Table 2 Outcome assessment

Variables	Measuring methods	Baseline (2022.9)	3 months after baseline (2022.12)	9 months after baseline (2023.6)	24 months after baseline (2024.9)	36 months after baseline (2025.9)
Anthropometric measures
Height	Stadiometer	√	√	√	√	√
Weight	Lever scale	√	√	√	√	√
Waist circumference	Tape	√	√	√	√	√
Hip circumference	Tape	√	√	√
Blood pressures	Digital sphygmomanometer	√	√	√
Body composition	Inbody770 (Biospace, USA)	√	√	√
Blood measures
Fasting glucose	Follow clinical standard protocols	√		√
Fasting insulin		√		√
Triacylglycerol		√		√
Total cholesterol		√		√
Low-density lipoprotein cholesterol		√		√
High-density lipoprotein cholesterol		√		√
Alanine aminotransferase		√		√	√	√
Liver ultrasound measures
Liver stiffness measurement	FibroScan HANDY (Echosense, France)	√	√	√	√	√
Controlled attenuation parameters	FibroScan HANDY (Echosense, France)	√	√	√	√	√
Physical fitness measure
20-m shuttle-run test	Conducted by trained physical health teacher	√		√	√	√
Behavioural measures
Students’ eating behaviour	Dietary quality questionnaire (DQQ) [20]	√		√
Students’ physical activity behaviour	Self-designed questionnaire [18]	√		√
Psychological status	Center for Epidemiologic Studies Depression Scale (CES-D Scale) [21] and Social Anxiety Scale for children (SASC) [22]	√		√
Sleep quality	Pittsburgh sleep quality index (PSQI) [23]	√		√

ISSN: 1745-6215