Using prophylactic antioxidants to prevent noise-induced hearing damage in young adults: a protocol for a double-blind, randomized controlled trial
© Gilles et al.; licensee BioMed Central Ltd. 2014
Received: 20 November 2013
Accepted: 21 March 2014
Published: 5 April 2014
During leisure activities young people are often exposed to excessive noise levels resulting in an increase of noise-induced symptoms such as hearing loss, tinnitus and hyperacusis. Noise-induced tinnitus is often perceived after loud music exposure and provides an important marker for overexposure as a temporary threshold shift that is often not experienced by the individual itself. As oxidative stress plays an important role in the pathogenesis of noise-induced hearing loss, the use of antioxidants to prevent hearing damage has recently become the subject of research.
This study proposes a randomized, double-blind, placebo-controlled crossover trial to assess the effects of a prophylactic combination of N-acetylcysteine (600 mg) and magnesium (200 mg) prior to leisure noise exposure in young adults. The primary outcome measure is the tinnitus loudness scored by a visual analogue scale (VAS). Secondary outcome measures are the differences in audiological measurements for the antioxidant treatments compared to placebo intake. Audiological testing comprising of pure tone audiometry including frequencies up to 16 kHz, distortion product otoacoustic emissions, transient-evoked otoacoustic emissions and speech-in-noise testing will be performed prior to and within 7 hours after noise exposure. By use of a mixed effects statistical model, the effects of antioxidants compared to placebo intake will be assessed.
As adolescents and young adults often do not use hearing protection while being exposed to loud music, the use of preventive antioxidant intake may provide a useful and harmless way to prevent noise-induced hearing damage in this population. Furthermore, when exposed to hazardous noise levels the protection provided by hearing protectors might not be sufficient to prevent hearing damage and antioxidants may provide additive otoprotective effects. Previous research mainly focused on occupational noise exposure. The present study provides a protocol to assess the usefulness of antioxidants during leisure noise activities.
The present protocol is registered at ClinicalTrials.gov: NCT01727492.
KeywordsNoise-induced hearing loss Antioxidants N-acetylcysteine Magnesium Adolescents ROS RNS Prevention Noise damage Randomized controlled trial
Adolescents and young adults frequently expose themselves to hazardous noise levels during social events or through personal listening devices. This has led to an increase of noise-induced symptoms such as noise-induced hearing loss (NIHL) and noise-induced tinnitus (NIT) over the last few years [1, 2]. Although 15% to 18% of the young population already experiences permanent NIT and the majority acknowledge the fact that loud music can damage the hearing, the use of hearing protection remains very low [3–5].
Oxidative stress plays a crucial role in the pathogenesis of NIHL and NIT. During excessive noise exposure, the outer hair cells endure metabolic depletion leading to accumulation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) which may ultimately lead to necrosis and apoptosis . While necrosis is a passive form of cell death, usually occurring after gross physical or chemical insult, associated with cell swelling and eventually causing cell rupture and loss of function, apoptosis is an active way of cell death which also occurs under normal metabolic circumstances . However, when apoptosis is ‘forced’ (for example as a consequence of noise exposure) and by this initiated at the wrong time, crucial healthy outer hair cells may die .
Under normal circumstances, the human cochlea contains molecules including vitamins, glutathione, enzymes and reactive transcription, which work together to form a complex and sophisticated defense mechanism against oxidative molecules . In cases of excessive noise exposure, the naturally occurring antioxidant systems may not render sufficient detoxifying effects, leading to the possible important role of antioxidant treatment after acoustic trauma [9, 10].
While antioxidant treatment for noise-induced hearing damage has often been the subject of animal research [11–17], well-performed human randomized controlled trials remain scarce. Examining the literature, two antioxidants have been mainly investigated as prophylactic prevention in animal and also some human studies: N-acetylcysteine (NAC) [18–21] and magnesium (Mg2+) [22, 23]. As oxidative stress triggers several cascades, it has been proven that a combination of antioxidants may provide better effects compared to a single antioxidant . Most human antioxidant studies have mainly investigated the effects during occupational noise exposure . Hence, the use of prophylactic antioxidants during recreational noise is unclear. As the use of hearing protection does not always render sufficient protection while exposed to hazardous noise levels, an addition of antioxidants may reduce noise-induced symptoms in young people.
The present article proposes a protocol for a clinical study consisting of a double-blind, randomized, placebo-controlled trial with a combination of NAC and Mg2+ as prophylactic antioxidants in adolescents during leisure noise exposure. The present report will follow the guidelines expressed by Consolidated Standards of Reporting Trials (CONSORT).
This study proposes a randomized, double-blind, placebo-controlled crossover trial. Participants need to spend at least 3 consecutive hours in an environment where loud music is played (>95 dB(A)) after taking either a combination of antioxidants (NAC and Mg2+) or a placebo, 1 hour prior to noise exposure. Each participant needs to repeat this procedure four times (interval between trials at least 4 days) and each participant will receive twice the antioxidants as well as the placebo. This study aims to investigate the combined effects of NAC and Mg2+ by use of four repeated treatments on the hearing of noise-exposed young adults.
We are recruiting students by advertisements sent by email and distributing information sheets around the University of Antwerp (Antwerp, Belgium). The aim is to always investigate groups (at least two individuals) of peers going out together and therefore attending the clinic within the time range of 7 hours after noise exposure. It is assumed that the pressure of peers also participating in the study will reduce the risk of drop-out. In addition, at the stage of enrolment it will be determined which occasions the subjects will attend and test moments will be scheduled. The aim is to plan all treatments within the range of 3 months in order to reduce the risk of drop-out.
Participants will receive an antioxidant combination of NAC and Mg2+, or a placebo drug (sugar pill). The antioxidant packets contain two pills of each 300 mg NAC and 100 mg Mg2+. Both pills, providing a total dose of 600 mg NAC and 200 mg Mg2+, should be taken orally 1 hour before noise exposure with a large glass of water. In 50%, the packets contain a placebo with an identical appearance and weight as the antioxidants. All participants (as well as the researchers) will be blinded to the order of antioxidants and placebo. The effects of NAC and Mg2+ at the respective doses of 600 mg and 200 mg will be analyzed as well as the effects of the combined product, for which no significant adverse effects have been reported in the past. Adverse events are defined as symptoms occurring after the administration of antioxidants or placebo that were not necessarily related to the intervention. Participants will receive a telephone number to report adverse events during the study and will be urged to come to the clinic in case of serious adverse events.
Permitted and prohibited concomitant treatments
The use of alcohol and tobacco is limited to a maximum of two alcohol consumptions and no to two cigarettes. Other psychoactive drugs are strictly prohibited.
The inclusion criteria are as follows:
Age between 18 and 28 years old (males as well as females).
Experience of temporary tinnitus after leisure noise exposure in the past scoring at least 5 or more on a visual analogue scale (VAS) for loudness.
Willing to attend an indoor/outdoor music event with mean noise levels ≥95 dB(A) LAeq, 60 min for at least 3 consecutive hours.
The exclusion criteria are as follows:
Middle ear pathologies such as otitis media and the perforation of the tympanic membrane and history of such pathologies.
Known allergies for NAC or Mg2+.
Adolescents using hearing protection.
Recreational noise exposure within 4 days prior to a study trial.
The production of the antioxidant and placebo packets as well as the randomization of the ABAB protocol will be performed by an independent pharmacist. The packets of antioxidants and placebos will be labeled with a number corresponding with the numbers on the forms. The randomization file will be put into a sealed envelope and retained in a safe at the pharmacy. The randomization table will not be available for assessment by anyone else involved in the study. At the end of the study the envelope will be requested.
Sample size calculation and statistical analyses
A power calculation was performed in order to make an estimation of the sample size needed to detect significant differences in the VAS between the experimental and control group by use of the software G-Power. A two-sided paired t-test for mean differences was performed in which an α-level of 0.05 and a nominal power of 80% were used. Assuming a minimal difference of clinical effect of 0.5 between the pre- and post-noise measurements on the VAS, a non-parametric calculation showed a requirement of 21 participants.
All data in this trial will be assessed with SPSS Statistics version 20.0 (SPSS, Chicago, IL, USA) and SAS version 9.1.3 (SAS, Cary, NC, USA). Because multiple correlated measurements will be performed in the same participants, a mixed effects model will be applied. This analysis is preferable over more traditional approaches such as repeated measures analysis of variance (ANOVA) because of the advantages to deal with missing values. The main analysis will be fully specified before unmasking.
The primary outcome measure will be the score on the VAS for tinnitus loudness, which will be measured pre-noise exposure (at the time of antioxidant intake) and 1 hour after noise exposure. The assumed expectation is a drop of 50% of the tinnitus loudness in the antioxidant group compared to the placebo group.
The secondary outcome measure is the evaluation of a significant difference in test results of the audiological testing (audiometry, otoacoustic emissions and speech-in-noise tests) when comparing the placebo results to the antioxidant trials.
A series of audiological tests (described in the following paragraphs) will be performed prior to and within 7 hours after each noise exposure.
Main outcome measure
Visual analogue scale (VAS)
Tinnitus loudness will be scored on a VAS from 0 to 10 (0, no tinnitus at all; 10, extremely loud tinnitus, could not possibly be louder), once at the time of antioxidant (or placebo) intake (1 hour prior to exposure) and once 1 hour after noise exposure. At both occasions the VAS will be undertaken in a quiet room.
Secondary outcome measures
Pure tone audiometry
Pure tone liminar audiometry will be performed according to the current clinical standards (International Organization for Standardization (ISO) 8253-1:2010) using a two-channel AC40 audiometer (Interacoustics, Assens, Denmark) in a silent room. Air conduction thresholds will be measured under headphones at 125 Hz, 250 Hz, 500 Hz, 1 kHz, 2 kHz, 3 kHz, 4 kHz, 6 kHz and 8 kHz. In addition, high frequency air conduction thresholds will be performed including 9 kHz, 10 kHz, 11,200 Hz, 12,500 Hz, 14 kHz and 16 kHz. When air conduction thresholds between 250 Hz and 4 kHz exceed normality levels of 20 dB HL, the bone conduction threshold will be measured on 250 Hz, 500 Hz, 1 kHz, 2 kHz, 3 kHz and 4 kHz in order to make a distinction between conductive and sensorineural hearing loss.
Transient-evoked otoacoustic emissions (TEOAEs) as well as distortion-product otoacoustic emissions (DPOAEs) will be performed. TEOAEs are elicited with click sounds presented at an intensity level of 80 dB SPL and recorded over a frequency range of 500 to 4,000 Hz. DPOAEs are elicited by use of a set of two pure tone frequencies (f1 and f2) closely spaced and presented simultaneously at a level of 55 dB SPL for f1 and 65 dB SPL for f2. The largest and most robust distortion product is 2f1-f2 and can be detected in almost all normal ears. The amplitude of 2f1-f2 is optimized by use of the frequency ratio f2/f1 = 1.22. DPOAEs will be recorded in the frequency region from 1 kHz to 6 kHz.
Speech-in-noise testing will be performed using the Leuven intelligibility sentence test (LIST)  in an adaptive procedure with noise at a fixed level of 65 dB SPL. Each list consists of ten sentences. Both ears will be tested separately by use of headphones. The procedure starts at a signal-to-noise ratio (SNR) of 0 dB meaning that speech and noise are presented with an equal loudness (65 dB SPL). Subsequently, the intensity level within a list of sentences varies in steps of 2 dB adaptively in a 1-down (when the keywords in the sentence are correctly repeated), 1-up procedure to determine the 50% correct identification point, which is called the speech reception threshold (SRT), expressed in dB SNR. Three conditions will be tested for both ears: sentences in steady-state noise, sentences in amplitude-modulated noise by a 10 Hz modulation and sentences in amplitude-modulated noise by a 15 Hz modulation.
Controlling the noise levels
In order to be able to respect the inclusion criterion of the minimal noise levels of 95 dB(A) LAeq, 60 min for 3 consecutive hours, all participants will be equipped with portable decibel meters (CEL350, Casella, Bedford, UK). The decibel meter can be fixed onto a shirt or trousers but participants must be aware that the microphone entrance should remain free from overhanging clothing.
Written consent will be obtained from each participant. This study protocol was approved by the ethical committee at Antwerp University Hospital in June 2012 with protocol number 12/18/172.
According to the Standard Protocol Items: Recommendations for Interventional Trials (SPIRIT) guidelines, the authors declare that data that break the blind will not be presented prior to the release of mainline results. The breaking of the blind will occur at the end of the study. A clinical article will be written on the primary (including also secondary) outcomes of the study and results will be disseminated regardless of the magnitude or direction of effect. The present trial is not industry-initiated. As such, there are no publication restrictions imposed by sponsors.
In addition, a full study report, anonymized participant-level dataset and statistical code for generating the results will be made publicly available no later than 3 years after the termination of the study for sharing purposes.
As adolescents and young adults often do not use hearing protection while being exposed to loud music, the use of preventive antioxidant intake may provide a useful and harmless way to prevent noise-induced hearing damage in this population. Furthermore, when exposed to hazardous noise levels the protection provided by hearing protectors might not be sufficient to prevent hearing damage and antioxidants may provide additive otoprotective effects. Previous research mainly focused on occupational noise exposure. The present study will assess the usefulness of antioxidants during leisure noise activities.
The authors acknowledge the limitation of not including blood samples in the present protocol in order to control for naturally varying Mg2+ levels in the individual. However, the investigators considered that it was very likely that the drop-out rate would be very high when participants needed to give a blood sample at every test moment, which would be eight in total. It is also assumed that it is the acute increase of NAC and Mg2+ prior to noise exposure that provides the extra otoprotective effects and not so much the naturally high or low levels.
To our knowledge, this study is the first to combine NAC and Mg2+ as prophylactic antioxidants for noise-induced tinnitus and noise-induced hearing damage in young people performed in a randomized, placebo-controlled trial. Furthermore, both participants and investigators will be blinded for the sequence of antioxidants and placebo. Thus, this would not influence on one hand the participants in rating the tinnitus loudness and on the other hand the investigators in performing the audiological testing, limiting investigator bias as far as possible.
To date, half of the needed participants have been included in the study. The procedure started in September 2012. The study is anticipated to be completed in April 2014.
AG is a third year PhD student at the University of Antwerp. BI is a seventh year medical student at the University of Antwerp. KW is a PhD statistician at the University Hospital Antwerp. PvdH is Head of the Department of Otorhinolaryngology and Head and Neck Surgery at the University Hospital Antwerp and Professor at the University of Antwerp.
Analysis of variance
Consolidated Standards of Reporting Trials
Distortion-product otoacoustic emission
Leuven intelligibility sentence test
Noise-induced hearing loss
Reactive nitrogen species
Reactive oxygen species
Standard Protocol Items: Recommendations for Interventional Trials
Speech reception threshold
Transient-evoked otoacoustic emission
Visual analogue scale.
The present research is financially supported by a TOP-BOF scholarship of the University of Antwerp.
- Henderson E, Testa MA, Hartnick C: Prevalence of noise-induced hearing-threshold shifts and hearing loss among US youths. Pediatrics. 2011, 127: e39-e46. 10.1542/peds.2010-0926.View ArticlePubMedGoogle Scholar
- Niskar AS, Kieszak SM, Holmes A, Esteban E, Rubin C, Brody DJ: Prevalence of hearing loss among children 6 to 19 years of age: the Third National Health and Nutrition Examination Survey. JAMA. 1998, 279: 1071-1075. 10.1001/jama.279.14.1071.View ArticlePubMedGoogle Scholar
- Gilles A, Van Hal G, De Ridder D, Wouters K, Van de Heyning P: Epidemiology of noise-induced tinnitus and the attitudes and beliefs towards noise and hearing protection in adolescents. PLoS One. 2013, 8: e70297-10.1371/journal.pone.0070297.View ArticlePubMedPubMed CentralGoogle Scholar
- Quintanilla-Dieck ML, Artunduaga MA, Eavey RD: Intentional exposure to loud music: the second MTV.com survey reveals an opportunity to educate. J Pediatr. 2009, 155: 550-555. 10.1016/j.jpeds.2009.04.053.View ArticleGoogle Scholar
- Gilles A, De Ridder D, Van Hal G, Wouters K, Kleine PA, Van de Heyning P: Prevalence of leisure noise-induced tinnitus and the attitude toward noise in university students. Otol Neurotol. 2012, 33: 899-906.PubMedGoogle Scholar
- Lynch ED, Kil J: Compounds for the prevention and treatment of noise-induced hearing loss. Drug Discov Today. 2005, 10: 1291-1298. 10.1016/S1359-6446(05)03561-0.View ArticlePubMedGoogle Scholar
- Henderson D, Bielefeld EC, Harris KC, Hu BH: The role of oxidative stress in noise-induced hearing loss. Ear Hear. 2006, 27: 1-19.View ArticlePubMedGoogle Scholar
- Kopke R, Allen KA, Henderson D, Hoffer M, Frenz D, Van de Water T: A radical demise. Toxins and trauma share common pathways in hair cell death. Ann N Y Acad Sci. 1999, 884: 171-191. 10.1111/j.1749-6632.1999.tb08641.x.View ArticlePubMedGoogle Scholar
- Ewert DL, Lu J, Li W, Du X, Floyd R, Kopke R: Antioxidant treatment reduces blast-induced cochlear damage and hearing loss. Hear Res. 2012, 285: 29-39. 10.1016/j.heares.2012.01.013.View ArticlePubMedGoogle Scholar
- Haase GM, Prasad KN, Cole WC, Baggett-Strehlau JM, Wyatt SE: Antioxidant micronutrient impact on hearing disorders: concept, rationale, and evidence. Am J Otolaryngol. 2011, 32: 55-61. 10.1016/j.amjoto.2009.09.002.View ArticlePubMedGoogle Scholar
- Fetoni AR, De Bartolo P, Eramo SL, Rolesi R, Paciello F, Bergamini C, Fato R, Paludetti G, Petrosini L, Troiani D: Noise-induced hearing loss (NIHL) as a target of oxidative stress-mediated damage: cochlear and cortical responses after an increase in antioxidant defense. J Neurosci. 2013, 33: 4011-4023. 10.1523/JNEUROSCI.2282-12.2013.View ArticlePubMedGoogle Scholar
- Fetoni AR, Ralli M, Sergi B, Parrilla C, Troiani D, Paludetti G: Protective effects of N-acetylcysteine on noise-induced hearing loss in guinea pigs. Acta Otorhinolaryngol Ital. 2009, 29: 70-75.PubMedPubMed CentralGoogle Scholar
- Somani SM, Husain K, Whitworth C, Trammell GL, Malafa M, Rybak LP: Dose-dependent protection by lipoic acid against cisplatin-induced nephrotoxicity in rats: antioxidant defense system. Pharmacol Toxicol. 2000, 86: 234-241. 10.1034/j.1600-0773.2000.d01-41.x.View ArticlePubMedGoogle Scholar
- Clifford RE, Coleman JK, Balough BJ, Liu J, Kopke RD, Jackson RL: Low-dose D-methionine and N-acetyl-L-cysteine for protection from permanent noise-induced hearing loss in chinchillas. Otolaryngol Head Neck Surg. 2011, 145: 999-1006. 10.1177/0194599811414496.View ArticlePubMedGoogle Scholar
- Coleman JK, Kopke RD, Liu J, Ge X, Harper EA, Jones GE, Cater TL, Jackson RL: Pharmacological rescue of noise induced hearing loss using N-acetylcysteine and acetyl-L-carnitine. Hear Res. 2007, 226: 104-113. 10.1016/j.heares.2006.08.008.View ArticlePubMedGoogle Scholar
- Kopke R, Bielefeld E, Liu J, Zheng J, Jackson R, Henderson D, Coleman JK: Prevention of impulse noise-induced hearing loss with antioxidants. Acta Otolaryngol. 2005, 125: 235-243. 10.1080/00016480410023038.View ArticlePubMedGoogle Scholar
- Kopke RD, Weisskopf PA, Boone JL, Jackson RL, Wester DC, Hoffer ME, Lambert DC, Charon CC, Ding DL, McBride D: Reduction of noise-induced hearing loss using L-NAC and salicylate in the chinchilla. Hear Res. 2000, 149: 138-146. 10.1016/S0378-5955(00)00176-3.View ArticlePubMedGoogle Scholar
- Lindblad AC, Rosenhall U, Olofsson A, Hagerman B: The efficacy of N-acetylcysteine to protect the human cochlea from subclinical hearing loss caused by impulse noise: A controlled trial. Noise Health. 2011, 13: 392-401. 10.4103/1463-1741.90293.View ArticlePubMedGoogle Scholar
- Lin CY, Wu JL, Shih TS, Tsai PJ, Sun YM, Ma MC, Guo YL: N-Acetyl-cysteine against noise-induced temporary threshold shift in male workers. Hearing Research. 2010, 269: 42-47. 10.1016/j.heares.2010.07.005.View ArticlePubMedGoogle Scholar
- Aruoma OI, Halliwell B, Hoey BM, Butler J: The antioxidant action of N-acetylcysteine: its reaction with hydrogen peroxide, hydroxyl radical, superoxide, and hypochlorous acid. Free Radic Biol Med. 1989, 6: 593-597. 10.1016/0891-5849(89)90066-X.View ArticlePubMedGoogle Scholar
- Bielefeld EC, Kopke RD, Jackson RL, Coleman JK, Liu J, Henderson D: Noise protection with N-acetyl-l-cysteine (NAC) using a variety of noise exposures, NAC doses, and routes of administration. Acta Otolaryngol. 2007, 127: 914-919. 10.1080/00016480601110188.View ArticlePubMedGoogle Scholar
- Attias J, Weisz G, Almog S, Shahar A, Wiener M, Joachims Z, Netzer A, Ising H, Rebentisch E, Guenther T: Oral magnesium intake reduces permanent hearing loss induced by noise exposure. Am J Otolaryngol. 1994, 15: 26-32. 10.1016/0196-0709(94)90036-1.View ArticlePubMedGoogle Scholar
- Attias J, Sapir S, Bresloff I, Reshef-Haran I, Ising H: Reduction in noise-induced temporary threshold shift in humans following oral magnesium intake. Clin Otolaryngol Allied Sci. 2004, 29: 635-641. 10.1111/j.1365-2273.2004.00866.x.View ArticlePubMedGoogle Scholar
- Le Prell CG, Hughes LF, Miller JM: Free radical scavengers vitamins A, C, and E plus magnesium reduce noise trauma. Free Radic Biol Med. 2007, 42: 1454-1463. 10.1016/j.freeradbiomed.2007.02.008.View ArticlePubMedPubMed CentralGoogle Scholar
- Le Prell CG, Johnson AC, Lindblad AC, Skjönsberg A, Ulfendahl M, Guire K, Green GE, Campbell KC, Miller JM: Increased vitamin plasma levels in Swedish military personnel treated with nutrients prior to automatic weapon training. Noise Health. 2011, 13: 432-443. 10.4103/1463-1741.90317.View ArticlePubMedPubMed CentralGoogle Scholar
- van Wieringen A, Wouters J: LIST and LINT: sentences and numbers for quantifying speech understanding in severely impaired listeners for Flanders and the Netherlands. Int J Audiol. 2008, 47: 348-355. 10.1080/14992020801895144.View ArticlePubMedGoogle Scholar
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